| Project/Area Number |
12671298
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Chiba University |
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Principal Investigator |
SAITOH Yukio Chiba University, Graduate School of Medicine, Lecturer, 大学院・医学研究院, 講師 (60261905)
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| Co-Investigator(Kenkyū-buntansha) |
SAITOH Takashi Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (50205655)
FUJISAWA Takehiko Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (80110328)
SEKINE Yasuo Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (70312957)
馬場 雅行 千葉大学, 大学院・医学研究院, 助教授 (00143305)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | lung transplantation / rat / CTLA-4 / donor specific transfusion / tolerance |
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| Research Abstract |
(1) The expressions of CTLA-4 and CD28 in the recipient spleen T cells and these on the recipient spleen T cell surface after donor specific transfusion (DST) from Brown Norway to Fisher 344 rats were investigated by FACS and a confocal laser microscope. CD28 expressed both in and on the T cells in the DST rats as well as untreated rats. On the other hand, although CTLA-4 did not express on the T cell surface before and after DST, intra-cellular expression of CTLA-4 was observed after DST in the 20 % of T cells. This result suggested that DST has some relationship to intra-cellular expression of CTLA-4 in T cells.
(2) DST 7 days before transplantation induced prolongation of graft rejection by pathology. Control group showed grade 4 rejection, while DST group showed grade 1. As a third party, DST from ACI to F344 was performed and BN to F344 lung transplantation was performed. Pathology revealed grade 3 to 4. This suggested that DST was donor specific.
(3) CTLA-4 expression on the surface of graft infiltrating T lymphocytes (GIL) was investigated in a time course by FACS. In an isograft model, CTLA-4 expression was not identified during the study period of 7 days. In an allograft control model, surface expression was observed at the 3^<rd> day postoperatively. This was consistent with the appearance of rejection by pathology. This expression kept until the 7^<th> day. In an allo-DST model, the surface expression was observed at the first day and maintained for 7days. In the spleen T cells, the same phenomenon was observed.
(4) The same phenomenon as (3) was observed in GIL.
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