Research on the cause of the Aortic Dissection

• Project/Area Number: 12671319
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Yokohama City University
• Principal Investigator: TAKANASHI Yoshinori Yokohama City University, 1st Surgery Professor, 第一外科, 教授 (70075505)
• Co-Investigator(Kenkyū-buntansha): IMOTO Kiyotaka Yokohama City University, 1st Surgery Sub Professor, 第一外科, 助教授 (40203335) ; UEMURA Satoshi Yokohama City University, 2nd Internal medicine Professor, 第二内科, 教授 (00128589)
• Project Period (FY): 2000 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: Dissectional Anevrhythm / Aortic Dissection / Matrix Metaroproteare / MMPs / Jmmvnohistochemistry / 解離生大動脈瘤 / マトリックスメタプロテアーゼ

Research Abstract

Aortic dissection are characterized by fragility of tunica media of the aorta. Matrix metalloproteinase (MMP) is the enzyme which degrades extracellular matrix of the aorta. The purpose of this study was to examine those enzymes in patients with acute aortic dissection and to demonstrate those mechanism. Enzyme-linked immunosorbent assay were used to measure the quantification of MMP-2, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-2 in acute aortic dissection (n=30), abdominal aortic aneurysm (n=12) and control (n=16). Low MMP-2 level and low TIMP level were found in AAD (p<0.001) and AAA (p<0.01). TIMP-2/MMP-2 ratio and TIMP-2/MMP-9 ratio were also low level in AAD (p<0.001) and AAA (p<0.001). MMP-2 ratio and low TIMP-2/MMP-9 ratio in acute aortic dissection were considered to enhance chronic breakage and degradation of extracellular matrix, to lead to fragility of tunica media and to play an important role in the onset of aortic dissection.