| Project/Area Number |
12671326
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
YOSHIMASU Tatsuya Wakayama Medical University Department of Thoracic and Cardiovascular Surgery Instructor, 医学部, 助手 (60316099)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAGUCHI Kazunari Wakayama Medical University Institute of Advanced Medicine Professor, 付置研究所, 教授 (60178548)
MATSUURA Nariaki Osaka University School of Allied Health Sciences Professor, 医学部・保健学科, 教授 (70190402)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Lung Cancer / Brain Metastasis / Integrin / Laminin |
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| Research Abstract |
The aim of this study was to clarify the roles of integrin and extracellular matrix (ECM) in the process of non-small cell lung cancer (NSCLC) brain metastasis. We established an in vivo model of brain metastasis of human NSCLC cell line EBC-1/original in athymic mice, and established highly brain metastatic subdone EBC-1/brain and highly bone metastatic subdone EBC-1/bone. Integrin expression of these subdones was evaluated by flowcytometry. In vitro cell attachment, migration and proliferation assays with ECMs were performed using these subdones. Expression of integrin alpha 3 subunit was higher in EBC-1/brain than in both EBC-1/original and EBC-1/bone. In vitro cell attachment, migration, and proliferation assays revealed that EBC-1/brain had higher affinity and higher reactivity to laminin than EBC-1/original and EBC-1/bone. Blocking of integrin a3b1 significantly (p<0.05) decreased brain metastasis by EBC-1/brain. Interaction of integrin alpha 3 beta 1 and laminin plays important roles in the process of brain metastasis of non-small cell lung cancer.
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