| Co-Investigator(Kenkyū-buntansha) |
HIDA Kazutoshi Hokkaido Univ., Grad. Sch. Of Med, Asso. Prof, 大学院・医学研究科, 助教授 (10238305)
TADA Mitsuhiro Hokkaido Univ., Institute for Genetic Medicine, Asso. Prof., 遺伝子病制御研究所, 助教授 (10241316)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
The Aim of this project is to disclose molecular pathological differences between spinal cord ependymomas and intracranial ependymomas, which show different biological malignancies.
1) We developed a yeast-based stop codon assay to detect truncating type mutations, in order to analyse molecular pathology in ependymomas (Am. J. Pathol.)
2) We developed an original DNA array consisting of 1300 genes. We analyzed 119 cancer cell lines including glial tumor cells with the array, and obtained successful results.
3) We analyzed 8 cases of spinal ependymoma and 8 cases of intracranial ependymoma with the DNA array, and identified several characteristic genes for spinal ependymoma (H-ras, M-ras, NCAM, ICAM-2, PDGFR-alpha, FGFR-3, p19INK4d) and intracranial ependymoma (VEGF, p57Kip2, MIP3alpha, MDM2, cyclin D3, TGFbeta2).
4) We found that spinal and intracranial ependymomas were well discriminated by pattern classification algorithms including cluster analysis and support vector machine with these expression profiles.
5) With more detailed analysis, we found that the two entities of ependymomas were best classified with STAT1 and MIP-3alpha using of a support vector machine. We are now analysing biological significance of these molecules in ependymomas.
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