ARYLSULFATASE A ACTIVITY/GENETIC ALTERATION AND RADIATION INDUCED LEUKOENCEPHALOPATHY
| Project/Area Number |
12671365
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | KYUSHU UNIVERSITY |
|---|
Principal Investigator |
IKEZAKI Kiyonobu FACULTY OF MEDICINE, KYUSHU UNIVERSIY, ASSOCIATE PROFESSOR, 医学部・附属病院, 講師 (10145360)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUI Masashi FACULTY OF MEDICINE, KYUSHU UNIVERSIY, PROFESSOR AND CHAIRMAN, 大学院・医学研究院, 教授 (10038713)
INAMURA Takanori FACULTY OF MEDICINE, KYUSHU UNIVERSIY, ASSISTANT PROFESSOR, 医学部・附属病院, 助手 (50274460)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
|
|---|
| Keywords | RADIATION THERAPY / LEUKOENCEPHALOPATHY / BRAIN / ARYLSULFATASE A / CHROMOSOME 22q13 |
|---|
| Research Abstract |
As one of the biological factors, radiation sensitivity plays a critical role in radiation induced leukoencephalopathy in adults. We hypothesized that arylsulfatase A activity might be involved in radiation sensitivity of the brain because recent studies disclosed that arylsulfatase A activity may related to demyelinating disease.
After receiving informed consent, blood arylsulfatase A activity was measured by artificial substrate method using Pnitrocathecole sulfate. Furthermore, DNA analysis at 22ql3 (E2400-PA2723) were performed to detect abnormality in arylsulfatase A gene. Arylsulfatase activity was compared between 20 normal volunteer and 20 patients with gliomas. Arylsulfatase A activity was within normal range (60- 144 mol/mg protein/h) and there was no significat difference between two groups. Arylsulfatase A activity was also compared between patients with leukoencephalopathy after radiation therapy and patients without leukoencephalopathy in gliomas. There was no significant difference between them.
DNA analysis on chromosome 22q13 also showed no gene abnormality. These results indicated that arylsulfatase A activity was not directly related to the induction of leukoencephalopathy after radiation therapy. We have finished a preliminary experiment for the effect of whole brain radiation on somatic neuronal stem cells. Further experiments might clarify the role of somatic neuronal stem cells on radiation induced leukoencephalopathy.
|
Report
(3 results)
Research Products
(9 results)
