| Project/Area Number |
12671424
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
MATSUMOTO Tomoko University of School of Medicine, Nagasaki University, Associate professor, 医学部, 助教授 (30239107)
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| Co-Investigator(Kenkyū-buntansha) |
TUKAZAKI Tomoo University of School of dentistry, Nagasaki University, Associate professor, 歯学部, 助教授 (50315230)
ENOMOTO Hiroshi University of School of Medicine, Nagasaki University, Assistant professor, 医学部, 助手 (90284679)
TSURUMOTO Toshiyuki University of School of Medicine, Nagasaki University, Assistant professor, 医学部, 助手 (60304937)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Insulin like growth factor / IGF binding protein / chondrocytes / Cartilage tissue / IGF-結合蛋白 |
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| Research Abstract |
Insulin like growth factor (IGF) promotes a differentiation and proliferation of bone and cartilage, and plays an important role in the metabolism of normal cartilage. Since the repair of the damaged cartilage tissue was impaired, we assume that the IGF action might be unusual in OA. IGF exists in the serum or in the tissue with binding proteins (IGFBPs), and chondrocytes produce tissue specific IGFBPs. Therefore, IGFBPs not only carry IGF to a target organ, but having an important role as a local regulation factor of IGF. We examined the IGFBPs produced by OA cartilage on protein and mRNA levels, and compared them with the normal cartilage.
1. OA cartilage was cultivated in DMEM containing 10 % FBS, and IGFBP was detected by the Western ligand blotting followed by the Immune-blotting method. t-RNA was extracted from the cartilage and the mRNA of each IGFBP was identified by RT-PCR. As a result, increased production of IGFBP-2,3,4 and 5 was found in OA cartilage.
2. The expression pattern of each IGFBP in the cartilage tissue of OA was examined by the immunohistochemistry and in-situ-hybridization method. In OA cartilage, the expression of each IGFBPs was increased comparing to the normal.
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