| Co-Investigator(Kenkyū-buntansha) |
TSUCHIDA Masahiro Yamaguchi University, School of Medicine, Research Associate, 医学部, 助手 (80325216)
SUGA Akinobu Yamaguchi University, School of Medicine, Associate Professor, 医学部, 助教授 (50243639)
高井 公雄 山口大学, 医学部・附属病院, 助手 (10284241)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
To investigate the possibility that healing process following repeated injury by non-physiological dialysate plays a significant role in the pathogenesis of peritoneal ultrastructural alteration, mediated by the production of cytokines and extracellular matrix proteins. Material & Methods: Using 1N NaOH, circular wounds of uniform surface area were made in monolayers of subconfluent rat peritoneal mesothelial cells and peritoneal fibroblasts. At 0, 24 and 72 hours following wounding, Changes in mRNA expression for TGF-bl, b-FGF, HGF, VEGF and FN were semi-quantified by reverse transcription-polymerase chain reaction (RT-PCR). Non-wounded monolayers of RPMC and RPFB were used as controls with m RNA expression being determined at the same time points. Result: RPMC: TGF-b1, HGF, b-FGF and FN m RNA gradually increased up to 72 hours post-wounding, 1. 5-fold, 1. 6-fold, 1. 3-fold and 2.1-fold of the control levels, respectively. A significant increase was only observed for TGF-b1 whilst VEGF showed the least change with time. RPFB: HGF, b-FGF, VEGF and FN mRNA expression were slightly suppressed compared to control levels up to 72 hours post-wounding. TGF-b1, however, increased marked above control expression by the end of the wound healing process.
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