Basic research of the gene therapy of testicular tumor cell

• Project/Area Number: 12671537
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kagawa University
• Principal Investigator: KAMANO Hiroshi Kagawa University, Health Center, Prof., 保健管理センター, 教授 (60284337)
• Project Period (FY): 2000 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: A-myb / B-myb

Research Abstract

The myb family consists of three members, the c-myb protooncogene and myb related genes A-myb and B-myb, all of which encode nuclear sequence-specific DNA-binding protein. C-myb proteins is expressed at high levels in most hematopoietic precursor cells and plays a crucial role in the proliferation and differentiation of these cells.
In the presence of DNA antisense B-myb oligo the proliferation rate of cell was significantly decreased on the fourth day. In the presence of mismatched DNA antisense B-myb oligo the proliferation 'rate of cell was not decreased. In the other hand, in the presence of morpholino antisense B-myb oligo the proliferation rate of cell was significantly decreased. In the presence of mismatched morpholin antisense B-myb oligo the proliferation rate of cell was not decreased.

Research Products

• [Publications] Kamano H, Sizmann J, Trauth K et al.: "Functional analysis of B-myb gene Expression of B-myb, alternative Splicing of B-myb gene(exon9a), and heat shock element dependent trans activation of the human HSP70 promotor"The First Waterfront Symposium of Human Genom Science. 1. 25 (2002)
• [Publications] Okutani Y, Kitanaka A, Tanaka T, Kamano H: "Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoietin-induced signaling pathway"oncogene. 20(45). 6643-6650 (2001)
• [Publications] Kubota Y, Tanaka T, Kitanaka A, Ohnishi H, Okutani Y, Waki M, Ishida T, Kamano H: "Src transduces erythropoietin-induced differentiation signals through phosphatidylinositd 3-kinase"EMBOJ. 20(20). 5666-5677 (2001)
• [Publications] Kamano H: "Photodynamic effects of gallium-metal porphyrin on human leukemia cells in combination with high-brightness LED light"Transplant.Proc.. 32(7). 2442-2443 (2000)