Electron microscopic immunohistochemical study of neuroendocrine vesicles in prostatic carcinomas
| Project/Area Number |
12671564
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | NIHON UNIVERSITY |
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Principal Investigator |
HIRANO Daisaku NIHON UNIVERSTY, SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (40228804)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Kiyoki NIHON UNIVERSTY, SCHOOL OF MEDICINE, PROFFESSOR, 医学部, 教授 (70059301)
ISHIDA Hajime NIHON UNIVERSTY, SCHOOL OF MEDICINE, ASSOCIATE PROFFESSOR, 医学部, 助教授 (70138452)
HACHIYA Takahiko NIHON UNIVERSTY, SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (40228482)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Prostate cancer / Neuroendocrine differentiation / Chromogranin A / Immunohistochemistry / Ultrastructure / Electron microscopic immunohistochemistry |
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| Research Abstract |
Seventy-four prostate cancer specimens obtained at radical prostatectomy and 25 prostate cancer specimens at autopsy cased by hormone refractory prostate cancer (HRPC) were studied the relationship between neuroendocrine (NE) differentiation status and prostatic carcinoma cells based on immunohistochemical analysis using an antibody against chromogranin A (CgA). These specimens were classified into 3 arms : 31 specimens from patients without hormone therapy (Group 1), 43 specimens from patients with response to androgen deprivation therapy for 3 to 6 months before radical prostatectomy (Group 2) and 25 autopsy specimens from patients with HRPC (Group 3). Staining of prostatic carcinoma was scored to the following grades : 0 = no staining, 1 = staining neoplastic cells < 10 %, 2 = staining tumor cells 10 to 20 %, 3 = staining neoplastic cells > 20 %. The mean staining score and standard deviation (SD) were 0.5 and 0.8 in Group 1, 0.7 and 0.6 in Group 2, and 1.7 and 1.0 in Group 3. There
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was statistical difference among these arms (p < 0.0001). NE differentiation status was increased in the patients with HRPC.
As to ultrastructural features of NE cells, they had electron dense granular vesicles in the cytoplasm. These granular vesicles were similar to those in NE cells in the other organ such as adrenal medulla. Ultrastructurally, NE cells were found in both benign glands and malignant tissues. However, NE cells in the malignant glands had cellular atypia : increase of chromatin I and nuclear bodies, and nuclear inclusions as seen in the conventional prostatic carcinomas. From these findings it appears that the biological features in the NE cells in malignant lesions differ from those in benign tissues. Based on electron microscopic immunohistochemical study we did not get specific findings in NE cells in both benign and malignant tissues.
In conclusions, the results of this study suggest that NE differentiation in malignant lesions is related to the progression of androgen-independent tumor cells and NE cells are biologically different from ones in benign tissues. Less
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(3 results)
Research Products
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