| Project/Area Number |
12671573
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
FURUTA Itsuko Hokkaido Univ., Grad. School of Med., Assist. Prof., 大学院・医学研究科, 助手 (70238682)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Seiichiro Hokkaido Univ., Grad. School of Med., Prof., 大学院・医学研究科, 教授 (60001898)
MINAKAMI Hisanori Hokkaido Univ., Grad. School of Med., Prof., 大学院・医学研究科, 教授 (40102256)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | OSTEOCLAST / UMBILICAL CORD BLOOD / BONE RESORPTION / ESTROGEN |
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| Research Abstract |
Optimal culture condition was assessed in inducing osteoclast from the human umbilical cord mononuclear cells (uMNC). The uMNC were cultured in three DMEM/F12 medium containing 10% fetal bovine serum (FBS), 20% horse serum (HS) or 2% BSA(BSA), with or without following substances ; M-CSF, IL-4, osteoclast differentiation factor, and 1 α, 25-dihydroxyvitamine D3 (VD). Multinucleated cells positive for tartrate-resistant acid phosphatase were efficiently induced in uMNC cultured with M-GSF added FBS or M-CSF added BSA in the presence of VD, or with M-CSF added HS in the presence of IL-4. Calcitonin-induced cAMP production was significantly increased in uMNC cultured with M-CSF added HS in the presence of VD or M-CSF added BSA in the presence of IL-4. Bone resorbing activity was increased in uMNC cultured with M-CSF added HS in the presence of VD. Estradiol (E2) did not stimulate cAMP production of in uMNC cultured with BSA containing M-CSF and IL-4. LPS-induced TNFα production was investigated in 3 uMNC with estrogen receptor gene polymorphism PP, Pp, and pp cultured with BSA containing M-CSF and IL-4 in the precence (+) or absence of E2 (-). The LPS-induced TNFα production ratio of E2 (+) uMNC- to - E2 (-) uMNC was 0.60±0.06 for uMNC with PP, 1.06±0.06 for uMNCwith Pp, and 1.44±0.12 for uMNC with pp. Thus, both the estrogen and its receptor gene polymorphism affected the LPS-induced TNFα production in UMNC.
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