In-vivo gene transfer into endometrial epithelial cells and endometrial stromal cells during pen-implantation period
| Project/Area Number |
12671591
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
MINOURA Hiroyuki Mie University, Hospital, Assistant Professor, 医学部附属病院, 講師 (70242964)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOYODA Nagayasu Mie University, Faculty of Medicine, Professor, 医学部, 教授 (40126983)
竹内 茂人 三重大学, 医学部, 助手 (00273368)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
|
|---|
| Keywords | embryonal / implantation endome trium / specific gene expression / gene transfer / endometrial epithelial cells / enometrial stromal cells |
|---|
| Research Abstract |
The greatly increased knowledge on preimplantation embryos and the molecular biology of the uterus have led to the development of new concepts on the implantation process. However, the exact molecular events in the endometrium induced by the embryonal implantation are still far from clear. It is important to identify the factors participating in the feto-maternal relationships around the time of embryo implantation. We identified up-or down regulated genes, such as LRG-21, neuropeptide Y, Ghrelin during embryonal implantation in mouse uterus using differential display method, subtraction RT-PCR method and gene tip method. To clarify the functions of these genes, we attempted to establish the method of in-vivo gene transfer into endometnial epithelial cells and endometrial stromal cells during pen-implantation period. We used reporter gene constructs to transfect mouse endometnium in vivo. We injected DNA-liposome complexes into the uterine lumen, endometnium and tail vessel of mice on day 0.5 〜5 of pregnancy and detected reporter gene activity 2 days later. Unfortunately, incorporation rates of plasmid into endometnial epithelial cells and endometnial stromal cells were less than 10%.
|
Report
(3 results)
Research Products
(12 results)
