| Project/Area Number |
12671634
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nihon University |
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Principal Investigator |
HAYAKAWA Satoshi Nihon Univ., Dept.Infect Dis.Cont, Associate Professor, 医学部, 助教授 (30238084)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tatsuo Nihon Univ., Dept Obstet.Gynecol., Professor, 医学部, 教授 (40167721)
CHISHIMA Fumihisa Nihon Univ., Dept Obstet.Gynecol., Assistant Professor, 医学部, 講師 (50277414)
ALEEMUZZAMAN Sheikh Nihon Univ., Dept Pathology, Research fellow, 医学部, 助手 (10277436)
NEMOTO Norimichi Nihon Univ., Dept Pathology, Professor, 医学部, 教授 (80096875)
HONDA Mitsuo National Inst.Infect., AIDS research Center, Diseases Research group director, エイズ研究センター, 第一研究グループ長 (20117378)
西成田 進 日本大学, 医学部, 講師 (70110963)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | HIV / decidua / placenta / chemokines / NK cell / NKT cell / G-CSF / γδ T cell / 垂直感染 / 脱落膜免疫細胞 / _γδT細胞 / CD56^<bright>LGL / IL-16 / SHIV / エイズ / 胎盤関門 / アポトーシス / カニクイザル / 母児感染 / CD4 / ケモカイン・レセプター |
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| Research Abstract |
1 We investigated placenta samples obtained from HIV positive mothers. We observed positive staining of HIVp24 in villous trophoblasts, extravillous trophoblasts and Hofbauer cells by immunohistochemical techniques, though their babies were not infected with HIV.
2 Presence of HIV provirus DNA was confirmed with PCR in antigenically separated placental cells.
3 Human choriocarcinoma cell line JEG-3 was susceptible to cell free HIV and HIV infected T cell lines. Both R5-HIV and X4-HIV replicate in JEG-3.
4 HIV infected JEG-3 by CD4 independent but CCR5 or CXCR-4 dependent manner.
5 Replication of HIV in choriocarcinoma cell lines were inhibited by various chemokines including MIP-1alpha and RANTES.
6 Freshly isolated placental trophoblasts and choriocarcinoma cell lines express functional chemokine receptors.
7 Chemokines regulate cell proliferation and hCG production of choriocarcinoma cell lines.
8 HIV infected choriocarcinoma cell line JEG-3 became susceptible to IL-2 activateddecidual CD56 positive large granular lymphocytes(LGL).
9 G-CSF and TGF-β produced by trophoblasts suppressed IL-2/IL-12 activation of CD56 positive LGL
10 CD56 positive LGL are susceptible to HIV but produce various chemokines and IL-16, Which possibly protect these cells and trophoblasts from HIVinfection
11 γδ T cells in deciduas produce large amount of TGF-β and IL-10 which possibly inhibit eradication of HIV infected cells by cytotoxic T cells.
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