| Project/Area Number |
12671643
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
HIRAI Yasuo CANCER INSTITUTE, DEPT. OF CELL BIOLOGY, ASSOCIATE, 癌研究所・細胞生物部, 研究員 (00260076)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | ENDOCERVICAL ADENQCARCINOMAS / TGF 13 RII / BAX / CHROMOSOMAL IMBALANCE / PTEN / MICROSATELLITE INSTABILITY / COMPARATIVE GENOMIC INSTABILITY / MULTISTEP CARCINOGENESIS / 子宮頚部腺癌 / TGFβRII / BAX / SMAD / CGH / 発癌過程 |
|---|
| Research Abstract |
We analysed the mutational status of the TGF β RII, BAX, and PTEN/MMAC1 genes as well as microsatellite instability (MI) in consecutive cases of endocervical adenocarcinoma of the uterus operated on at our Institute. To examine copy number losses and/or amplifications at the chromosomal region bearing all the genes, we used comparative genornic hybridization (CGH) analysis. CGH analysis may provides a genome-wide overview about tumor-associated genomic imbalances. Among the tumors, a few showed, the MI+ phenotype. Also, few tumors demonstrated a significant mutation with a definite amino acid change in PTEN/MMAC1 gene. CGH analysis demonstrated that a few tumors showed chromosomal copy number losses and/or amplifications, whereas there are no common chromosomal foci showing losses or amplifications among the tumors analysed so far. The role of TGF β RI and BAX genes may be limited as a target gene of MI+ phenotype in endocervical adenocarcinoma, because only a few tumors demonstrated MI+ phenotype in these tumors.
Additional explorations are needed to elucidate the cytogenetic mechanisms of multistep carcinogenesis in endocervical adenocarcinomas of the uterus.
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