| Project/Area Number |
12671740
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
KONDO Satoshi Nagoya City University Medical School, Assistant Professor, 医学部, 講師 (50234935)
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| Co-Investigator(Kenkyū-buntansha) |
TOYAMA Tatsuya Nagoya City University Medical School, Research Associate, 医学部, 助手 (30315882)
IWASE Hirotaka Nagoya City University Medical School, Associate Professor, 医学部, 助教授 (40211065)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | ING1 / tumor suppressor gene / neuroblastoma / 分子生物学 / ING1遺伝子 |
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| Research Abstract |
The candidate tumor suppressor gene ING1 was isolated, based on the observation that down regulation og the gene promotes growth in soft agar and focus formation in vitro and tumor formation in vivo. ING1 encodes a family of alternative spliced, nuclear, cell-cycle-regulated proteins, some of which cooperate with p53 in the negative regulation of cell growth by transcriptionally activating p21WAF1/Cip1. To examine whether ING1 was involved in the development of neuroblastoma, we screened for mutation in 80 primary neuroblastoma tissues and 6 cell lines, and stdied the expression level of ING1 mRNA in 20 neuroblastoma tissues and 6 cell lines. No mutations were detected, however, marked decreases in ING1 mRNA expression were seen in all 9 primary neuroblastomas and 6 neuroblastoma cell lines examined. These observations show that mutation of the ING1 gene is rare in neuroblastoma, however, suggest that down regulation of ING1 might contribute to the genesis and development of neroblastoma.
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