| Project/Area Number |
12671748
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | Kagawa Medical University |
|---|
Principal Investigator |
SUZUKI shigehiko Kagawa Medical University, Plastic and Reconstruction Surgery, PROFESSOR (2000-2001), 医学部, 教授 (30187728)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MUNEUCHI gan Kagawa Medical University, Plastic and Reconstruction Surgery, ASSISTANT PROFESSOR (2002), 医学部, 講師 (40335873)
KAWAZOE takeshi Kagawa Medical University, Plastic and Reconstruction Surgery, RESEARCH ASSISTANT (2000-2002), 医学部附属病院, 助手 (20325351)
ITO osamu Kagawa Medical University, Plastic and Reconstruction Surgery, ASSOCIATE PROFESSOR (2000-2001) (90243766)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | WOUND HEALING / SKIN / ARTIFICIAL SKIN / SKIN MODEL / FIBROBLAST / KERATINOCYTE / NITRIC OXIDE / COLLAGEN SPONGE / 表皮 / 分化 |
|---|
| Research Abstract |
While nitric oxide (NO) released under physiological condition regulates blood flow and blood pressure, excess NO exerts cytotoxic effects on living tissues as radical. Except for regulation of blood flow, various functions of NO have been reported. However, its effect on would healing has been unclear. We studied the effect of NO on would healing.
As it is difficult to reproduce a reaction of the living body using in vitro model, we developed a hybrid cultured skin with both epidermal and dermal components as an experimental model.
Different from a simple cultured mode, this new cultured skin has 3 dimensional structure like a living skin.
Administration of NOS (NO synthase) inhibitor did not have effect on differentiation of keratinocytes in the 3-D cultured skin model.
Proliferation of cultured fibroblasts was accelerated by NO donor (NOC-18) dose-dependently, but suppressed by NOS inhibitor (L-NAME) for the first 24 hours. Proliferation of fibroblasts was, however, suppressed by high dose of NO donor after 72 hours.
Proliferation of cultured keratinocytes was accelerated by No donor dose-dependently but suppressed by NOS inhibitor for the first 72 hours. Proliferation of keratinocytes was suppressed by high dose of No donor after 120 hours. Keratinization was observed only when NOS inhibitor had been administered.
Additional studies are required to conclude the effect of NOS inhibitor on differentiation of keratinocytes.
|
