Search for the expression of estrogenic action of dental materials
| Project/Area Number |
12671867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
NEGISHI Hideyuki Kanagawa Dental College, Dental Bioengineering, Instructor, 歯学部, 講師 (60121026)
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| Co-Investigator(Kenkyū-buntansha) |
KURATA Shigeaki Kanagawa Dental College, Dental Materials, Assistant Professor, 歯学部, 助教授 (20104333)
FUJIHARA Tsutomu Kanagawa Dental College, Dental Bioengineering, Assistant Professor, 歯学部, 助教授 (50084778)
KAWASE Toshio Kanagawa Dental College, Dental Bioengineering, Professor, 歯学部, 教授 (30084784)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Dental Materials / Endocrine Disruptors / Estrogenic Activity / MCF-7 / Bisphenol A / Sealant / Polycarbonate |
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| Research Abstract |
Recent studies have been saised about the possibility that one of estrogenic chemicals, bisphenol A (BPA) might be leached out of dental sealants. This study aimed to identify and quantify BPA and other components released from sealants and dental polycarbonate materials in vitro. The sealants tested were not shown to have released BPA analyzed by a liquid chromatography. The three dental polycarbonate materials contained the amounts of 28.8 to 203 μg of BPA pre gram of pellet. But the content of BPA of dental PC material extracted with PBS(-) was less than μg level per gram dental PC material.
The estrogen-sensitive MCF-7 breast canser cells was a useful tool that may estimate an estrogenic chemicals (environmental estrogens, xenoestrogens). When incubated with MCF-7 cultured with dextran-charcoal treated FCS and phenol red-free medium, BPA, 17 β-estradiol, diethylstilbestrol (DES) and ethynylestradiol promoted cell proliferation that was assayed the DNA content by DABA method, and induced the expression of progesterone receptor mRNA that was analyzed by RT-PCR. The extracts of dental sealants and dental PC materials with PBD(-) were not affected the proliferation and the expression of progesterone receptor of MCF-7.
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Report
(4 results)
Research Products
(12 results)
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[Publications] Matsuzawa,M., Muroi,T., Uchimura,N., Kurata,S., Umemoto,K., Kawase,T.: "Determination of Bisphenol A in Dental Resin Materials by Liquid Chromatography/Mass Spectrometry"The J of the Kanagawa Odontological Society. 35-4. 201-214 (2000)
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[Publications] Saito,M., Iwase,M., Maslan,S., Nozaki,M., Yamauchi,M., Handa,K., Takahashi,O., Sato,S., Kawase,T., Teranaka,T., Narayanan,A.S.: "Expression of Cementum-derived Attachment Protein in Bovine Tooth Germ During Cementogenesis"Bone. 29. 242-248 (2001)
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[Publications] Muroi,T., Yamaguchi,M., Suga,H., Matsuzawa,M., Kurata,S., Negishi,H., Fujihara,T., Uchimura,N., Kawase,T.: "Content of Bisphenol A in Polycarbonate Used in Dentistry, and its Leaching Behavior"The J of the Kanagawa Odontological Society. 37-2/3. 76-82 (2002)
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[Publications] Deguchi,S., Tago,K., Yamashita,O., Sato,S., Koke,Y., Sugaya,A., Iijima,Y.,Kawase,T.: "Effect of Enamel Matrix Derivative on Migration of Human Periodontal Ligament Fibroblasts"The Bulletin of Kanagawa Dental College. 30-1. 27-31 (2002)
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[Publications] Tomiyama,K., Mukai,Y., Okawa,S., Tsubota,Y., Negishi,H., Fujihara,T., Kawase,T., Ueda,M., Nakagawa,S., Teranaka,T.: "Acidic Resistance of FTLA Treatment as a Medicament for Dentin Hypersensitivity -Measurement of Dentin Permeability in vitro-"The J of Conservative Dentistry. 45-4. 688-695 (2002)
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[Publications] Saito,D., Minamida,G., Izukuri,K., Tani-Ishii,N., Kato,Y., Ozono,S., Kawase,T., Teranaka,T., Koshika,S.: "Effect of Pubertal Treatment with Phisphenol A and Bis-GMA on Sex Hormone Level in Male Rats"Environmental Sciences. 10-1. 55-61 (2003)
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