| Project/Area Number |
12672019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Nihon University |
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Principal Investigator |
MAEDA Takahide Nihon University, School of Dentistry at Matsudo, Professor, 松戸歯学部, 教授 (70130599)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Takehiko Nihon University, School of Dentistry at Matsudo, Lecturer, 松戸歯学部, 講師 (40328761)
朝田 芳信 日本大学, 松戸歯学部, 助教授 (20184145)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | embryo brain kinase / in situ hybridization / tooth development / culture of tooth germ / mouse / Ebk / Embryo brain Kinase / tooth development |
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| Research Abstract |
We tried first in situ hybridization analysis using specific probes of target genes, however we could not detect good hybridization signals. Therefore we changed target gene to embryo brain kinase (Ebk), which was known little its role in mouse oral development.
Ebk/MDK1 gene belongs to receptor tyrosine kinases (RTKs) family has been shown to be closely related to the Eph/Eck/Elk subfamily. A study was conducted to investigate the role of Ebk/MDK1 in oral development of early mouse embryogenesis. In this study, RT-PCR analysis identified presence of Ebk/MDK1 in the region of first molar at El5 mouse. In situ hybridization analysis in developing oral field from E12 to E16 revealed that Ebk/MDK1 expressed in the mesenchyme underlying the dental epithelium of both of the first molars and incisors, as well as in the mesenchyme of developing palate tongue, lip and nosal cartilage. Moreover expression of Ebk/MDK1 in the first molars revealed remarkable differences between the mesial and distal parts of the tooth germs during early stage of tooth development. Culture experiment of tooth germs of the first molar using antisense Ebk/MDK1 oligonucleotides block showed that tooth germs treated with antisense oligonucleotide did not show normal development with formation of ameloblast and enamel organ. Our findings suggest that Ebk/MDK1 may play an important role in tooth development.
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