| Co-Investigator(Kenkyū-buntansha) |
ISHII Rie Division of Food Chemistry, Saitama Institute of Public Health, Research Scientist, 水食品, 主任
DAIKONYA Akihiro NIHON UNIVERSITY, COLLEGE OF PHARMACY, ASSISTANT, 薬学部, 助手 (60328763)
松崎 桂一 日本大学, 薬学部, 助手 (20229454)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Research Abstract |
We examined Bidens parviflora with NO production inhibitory effects and prostaglandin E_2 (PGE_2) inhibitory effect toward the macrophage secretions which had a relation in antiallergy and chronic inflammation. Chinese folk medicine, Bidens parviflora (Compositae) has been used as a traditional antipyretic and anti-rheumatic medicine. Sixty-one compounds isolated from this whole plant were examined for anti-allergic actively toward nitric oxide (NO) production by a murine macrophage-like cell line, RAW264.7, activated with lipopolysaccharide (LPS) and interferon-γ(IFN-γ) and histamine release from rat peritoneal mast cells in vitro. We isolated five new polyacetylene glucosides, twenty-five flavonoides containing two new compounds, a new dimeric caffeic acid derivative, two new caffeic acid esters, two caffeic acid derivatives, two cinnamic acid derivatives, two new neolignans, a cyclopentene glycoside having ten carbon chain, eight quinic acid esters, a benzyl alcohol glycoside, a naphthopyrone glycoside, carotinoids, a diterpene, sterols and myo-inositol.
Among five polyacetilene glucosides, four exhibited strong inhibitory activities toward NO production and, about effect to give to NO product performance of a macrophage of these amalgam, restraint was recognized as a result of having examined action of an examination drug at having been revitalized with IFN-γ and LPS. In the flavonoids, NO product inhibitory activities were recognized by flavones, flavonols and aurones which had two hydroxyl groups to 3', 4' -position. In addition, NO product inhibitory activities were recognized, by a caffeic acid dimer, a caffeic acid ester of methyl quinic acid. We investigated the inhibitory effects of isolated compounds on prostaglandin (PG) E_2 production in LPS-activated macrophages. About sucrose p-hydroxylcinnamic acid esters and caffeic acid derivatives, PGE_2 production inhibitory activities were recognized by several compounds.
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