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Control of vancomysin-resistant enterococci in Japan and the study of a combination therapy

Research Project

Project/Area Number 12672214
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用薬理学・医療系薬学
Research InstitutionOkayama University

Principal Investigator

KARIYAMA Reiko  Okayama University Hospital, Assistant, 医学部附属病院, 助手 (40112148)

Co-Investigator(Kenkyū-buntansha) KUMON Hiromi  Okayama University Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (30144760)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsvancomycin-resistant enterococci / VRE / infection control / detection method / PCR assay / molecular epidemiology / transposon / combination therapy / VRE / バンコマイシン耐性 / 腸球菌
Research Abstract

The first report in Japan of a vaneomycin-resistant enterococcal clinical isolate was in 1996. Although outbreaks of VariA-type or VanB-type vaneomycin-resistant enterococci (VRE) at some Japanese hospitals were reported, infections or colonizations due to VRE have been rare in Japan until May 2003. In 1998, a patient colonized with VanA-type Enterococcus faecium (VREF) was hospitalized at the Okayama University Hospital (Okayama, Japan). Since then, to control one of the most important nosocomial pathogens, we have studied on detection of VRE, molecular epidemiology of VRE and a combination therapy for VRE.
We have developed a simple and reliable multiplex PCR assay for surveillance isolates of VRE. Seven primer sets targeting the genes vanA, vanB, vanCl, vanC2/C3, Enterococcus faecalis-specific, Enterococcus faecium-specific and rrs(l6SrRNA) were used in one reaction tube. The optimized multiplex PCR assay is an attractive alternative to the other methods since it provides simpler and … More more accurate analysis of the molecular epidemiology of VRE isolates.
We identified a Tn1546-like (type2) element in VREF isolated from hospitalized patients in Japan. The Tn1545-like (type2) element has previously been associated with VREF isolated from pig feces in Europe. So far, four VRE isolates in Japan possessed the unique molecular variations characteristic of Tn1546-like (type2) element, the presence of an IS1216V-IS3-like element in the left end of Tn1546and a point mutation (G to T) at position 8234 in the essential vanX gene of Tn1546.
Optical therapy for serious enterococcal infections requires the combination of a cell wall-active agent with an aminoglycoside (most commonly gentamicin) for synergistic killing. In our studies, the combination of ampicillin and arbekacin demonstrated inhibitory activity against VRE possessing aac(6')-aph(2'') or aph(2'')-Id genes. This combination may prove useful in treating infections caused by multiresistant enterococci, where treatment options are limited. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Reiko Kariyama et al.: "Simple and reliable multiplex-PCR assay for surveillance isolates of vancomycin-resistant enterococci"Journal of Clinical Microbiology. 38. 3092-3095 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Vivek Kak et al.: "Efficacy of ampicillin plus arbekacin in experimental rabbit endocarditis caused by an Enterococcus faecalis strain with high-level gentamicin resistance"Antimicrobial Agents and Chemotherapy. 44. 2545-2546 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Vivek Kak et al.: "In-vitro synergistic activity of the combination of ampicillin and arbekacin against vancomycin-and high-level gentamicin-resistant Enterococcus faecium with the aph(2")-Id gene"Diagnostic Microbiology and Infectious Disease. 37. 297-299 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Reiko Kariyama et al.: "Identification of a Tn1546-like (type 2) element in vancomycin-resistant Enterococcus faecium isolated from hospitalized patients in Japan"Antimicrobial Agents and Chemotherapy. 45. 992-993 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sameer Elsayed et al.: "Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp"Journal of Clinical Microbiology. 39. 2367-2368 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 狩山玲子: "VRE感染症に対する併用療法の検討"日本臨床. 59. 733-738 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kenneth Van Horn et al.: "Evaluation of 15 motility media and a direct microscopic method for detection of motility in enterococci"Journal of Clinical Microbiology. 40. 2476-2479 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 狩山玲子: "バンコマイシン耐性腸球菌(VRE)サーベイランスに有用なmultiplex-PCR法"Infection & Technology. 6. 10-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 公文裕巳編: "バンコマイシン耐性菌戦略Q&A"医薬ジャーナル社. 139 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Reiko Kariyama et al.: "Simple and reliable multiplex-PCR assay for surveillance isolates of vancomycin- resistant enterococci."Journal of Clinical Microbiology. 38. 3092-3095 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Vivek Kak et al.: "Efficacy of ampicillin plus arbekacin in experimental rabbit endocarditis caused by an Enterococcus faecalis strain with high-level gentamicin resistance."Antimicrobial Agents and Chemotherapy. 44. 2545-2546 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Vivek Kak et al.: "In-vitro synerglstic activity of the combination of ampicillin and arbekacin against vancomycin- and high-level gentamicin-resistant Enterococcus faecium with the aph(2'')-Id gene."Diagnostic Microbiology and Infectious Disease. 37. 297-299 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Reiko Kariyama et al.: "Identification of a Tn1546-like (type 2) element in vancomycin-resistant Enterococcus faecium isolated from hospitalized patients in Japan."Antimicrobial Agents and Chemotherapy. 45. 992-993 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sameer Elsayed et al.: "Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp."Journal of Clinical Microbiology. 39. 2367-2368 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Reiko Kariyama et al.: "Combination therapy against vanconiycin-resistant enterococci."Nippon Rinsho. 59. 733-738 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kenneth Van Horn et al.: "Evaluation of 15 motility media and a direct microscopic method-for detection of motility in enterococci."Journal of Clinical Microbiology. 40. 2476-2479 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Relko Kariyama et al.: "Multiplex PCR for detection of vancomycin-resistant enterococci."Infection & Technology. 6. 10-11 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiromi Kumon et al.: "Strategies against vancomycin-resistant enterococci (Q & A)."Book : Iyakuja-narusha. 139 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kenneth Van Horn et al.: "Evaluation of 15 motility media and a direct microscopic method for dete ction of motility in Enterococci"Journal of Clinical Microbiology. 40.7. 2476-2479 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 狩山玲子: "検査とその周辺「バンコマイシン耐性腸球菌(VRE)サーベイランスに有用なmultiplex-PCR法」"Infection & Technology. 6. 10-11 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Reiko Kariyama: "Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci"Journal of Clinical Microbiology. 38・8. 3092-3095 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Vivek Kak: "In-vitro synergistic activity of the combination of ampicillin and arbekacin againet vancomycin-and high-level gentamicin-resistant Enterococcus faecium with the aph(2")-Idgene"Diagnostic Microbiology and Infectious Disease. 37・4. 297-299 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Reiko Kariyama: "Identification of a Tn1546-like(type2) element in vancomycin-resistant Enterococcus faecium isolated from hospitalized patients in Japan"Antimicrobiol Agents and Chemotherapy. 45・3. 992-993 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 狩山玲子: "VRE感染症に対する併用療法の検討"日本臨床. 59・4. 733-738 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sameer Elsayed: "Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp."Journal of Clinical Microbiology. 39・6. 2367-2368 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 狩山玲子(分担): "バンコマイシン耐性菌戦略Q&A"医薬ジャーナル社(公文裕巳編). 139 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 公文裕巳(分担): "バンコマイシン耐性菌戦略Q&A"医薬ジャーナル社(公文裕巳編). 139 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kariyama Reiko: "Simple and reliable multiplex PCR assay for surveillance isolater of vancomycin-resistant enterococci"Journal of Clinical Microbiology. 38・8. 3092-3095 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kariyama Reiko: "Identification of a Tn1546-like (type 2) element in vancomycin-resistant Enterococcus faecium isolated from hospitalized patients in Japan"Antimicrolial Agents and Chemotherapy. 45・3. 992-993 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 狩山玲子: "VRE感染症に対する併用療法の検討"日本臨床. 59・4. 733-738 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 公文裕巳(分担): "バンコマイシン耐性菌戦略Q&A"医薬ジャーナル社(公文裕巳 編). 139 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 狩山玲子(分担): "バンコマイシン耐性菌戦略Q&A"医薬ジャーナル社(公文裕巳 編). 139 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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