| Project/Area Number |
12672231
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
YAMAMOTO Akira Faculty of Pharmaceutical Sciences, Kyoto Pharmaceutical University, Professor, 薬学部, 教授 (00166779)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA-OKA Tkuya Faculty of Pharmaceutical Sciences, Kyoto Pharmaceutical University, Associate Professor, 薬学部, 助教授 (00247785)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Gastrointestinal absorption / Oral administration / Absorption enhancement / Drug efflux / Transporter / P-glycoprotein / Absorption enhancer / Pharmaceutical excipient / P糖タンパク |
|---|
| Research Abstract |
The effects of various surfactants and polyethylene glycol on the intestinal transport of rhodamine123, a P-gp substrate, were examined by an in vitro diffusion chamber system. The jejunal serosal-to mucosal transport (Jsm) of rhodamine123 was greater than its mucosal-to serosal transport (Jms). Nonionic surfactants (Cremophor EL, Tween80 and LM) reduced the Jsm/Jms ratio of rhodamine123, whereas its ratio was not influenced in the presence of cationic surfactant (C_<16>TAB) and anionic surfactant (SDS). Therefore, these findings suggested that charge of surfactants is possibly related to the action of these surfactants on the intestinal absorption of P-gp substrates. Polyethylene glycols with various molecular weights inhibited the Jsm flux of rhodamine123 across the rat jejunum membrane.
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