| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Research Abstract |
We investigated the effect of the different intensities of treadmill running training for 4 and 8 weeks on the co-localization of vasopressin (AVP) with corticotropin-releasing factor (CRF) in the hypothalamic paraventricular nucleus (PVN) of rats. Six-week-old male rats were divided into control (CTL) and 3 trained groups ran at 3 different treadmill-running speeds that were 10 m/min (10R), 20 m/min (20R) and 30 m/min (30R). The duration, frequency, and periods of exercise training were 30 min, 5 days/weeks, and 4 and 8 weeks, respectively. The double label immunofluorecent staining for CRF and AVP was performed and the number of neurons contained both CRF and AVP immunoreactivity was counted in PVN. The adrenal weights, which were weighed on the 2nd day after 4 and 8 weeks of training, were significantly heavier in 30R than in CTL. A few neurons contained both CRF and AVP immunoreactivity were found in CTL and 3 trained groups, 10R, 20R and 30R on the 2nd day after 4 and 8 weeks of training. However, there were no significant differences in the number of those neurons among CTL and 3 trained groups. As reported previously, by using the same staining that we performed in this study, we observed that the PVN neurons contained both CRF and AVP were much more numerous in adrenalectomized rats than in normal rats. This result supported that the double label immunofluorecent staining we performed in this study was appropriate. To judge from these results, it was suggested that the enhanced co-localization of AVP in the CRF neurons of PVN could not occur with intensive running training (30m/min speed) which caused the increase in adrenal weight.
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