Molecular Design and Evaluation of Artificial Gene Function-Regulating Agents in the Post-Genome Era
| Project/Area Number |
12680599
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Keio University |
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Principal Investigator |
TOSHIMA Kazunobu Keio University, Applied Chemistry, Associate Professor, 理工学部, 助教授 (60217502)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Post-Genome / DNA / DNA Cleavage / Light / Intercalator / Carbohydrate / Hybrid / Cytotoxicity / ハイブリッド / エンジイン / ネオカルチノスタチン / ハイブリッド分子 |
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| Research Abstract |
The development of photochemical DNA cleaving agents, which selectively cleave DNA by irradiation with a specific light under mild conditions and without any additives such as metals and reducing agents, is very interesting from a chemical and biological standpoint and offers considerable potential in medicine especially in the post-genome era. Furthermore, photodynamic therapy using a photosensitizing drug has recently emerged as a promising modality against cancer and allied diseases. In this project, several artificial anthraquinone-carbohydrate, 2-phenylquinpline-carbohydrate, quinoxaline-carbohydrate, and β-carboline-carbohydrate hybrid molecules were designed and synthesized. Furthermore, it was found that they had light-induced DNA-cleaving activity with DNA base or sequence selectivity. In addition, the quinoxaline-carbohydrate hybrids completely kill HeLa S3 cells upon UV irradiation while no action occurs without light.
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Report
(3 results)
Research Products
(6 results)
