Dopamine-serotonin interaction in the rat striatum during lever-pressing for food reward : high-speed in vivo voltammetric measurement of dopamine and serotonin release
| Project/Area Number |
12680782
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Juntendo University |
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Principal Investigator |
NAKAZATO Taizo Juntendo University School of Medicine, Department of Physiology, Assistant professor, 医学部, 助手 (80155697)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Dopamine / Serotonin / Release / Voltammetry / Learning / Reward / Striatum / Rat |
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| Research Abstract |
Rat was trained for the lever-press for food reward. Striatal dopamine (DA) and serotonin (5-HT) release was measured every 250 ms. After cue (tone), rat could get a pellet if the lever was pressed. Tone was applied every 7 s, totally 20 times in a session. Six sessions were performed every 30 min. After 20-30 experimental days, signal currents were analyzed.
DA current decreased temporally just after the tone stimulation, but it increased until around the lever-press. Then, DA current decreased again. Conversely, 5-HT current increased temporally after the tone stimulation, but soon it decreased. Then, 5-HT current seemed to return to the basal levels. PCPA (5-HT synthesis inhibitor) treatment diminished the temporal suppression of DA current and the increase around at the lever-press a-MPT (DA synthesis inhibitor) did not affect 5-HT release From these findings, it was concluded that the temporal suppression of DA release is caused by inhibitory 5-HT neuron, and that DA increase around at the lever-press may be partly facilitated by 5-HT neuron.
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Report
(4 results)
Research Products
(13 results)
