| Project/Area Number |
12793010
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
TAKAGAKI Keiichi Hirosaki University, School of Medicine, Professor, 医学部, 教授 (70163160)
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| Co-Investigator(Kenkyū-buntansha) |
KAKIZAKI Ikuko Hirosaki University, School of Medicine, Research Associate, 医学部, 助手 (80302024)
YOSHIHARA Syuichi Hirosaki University, Surgery School of Medicine, Lecture, 医学部, 講師 (00261454)
KATO Yoji Hirosaki University, Faculty of Education, Professor, 教育学部, 教授 (20194863)
IWAFUNE Mito Hirosaki University, School of Medicine, Research Associate, 医学部, 助手 (80312487)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2002: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2001: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Proteoglycan / Cartilase / Salmon / プリテオグリカン / 新素材 / 炎症性腸疾患 / 癒着防止剤 / プロテオグリカンシート / 老人介護 |
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| Research Abstract |
(Function of Proteoglycan)
1. Malaria
I went to study to Penn. State University College of Medicine and did a joint research with Prof. Channe Gowda concerning the exact structure of the proteoglycan that inhibits the adhesion of Plasmodium falciparum infected red blood cells in human placenta. As a result, the chondroitin 4-sulfate chain of proteoglycan showed inhibitory activity.
2. Ulcerative colitis
We examined the effect of orally administered chondroitin sulfate proteoglycan(PG) against the dextran sodium sulfate(DSS) induced experimental colitis of rats. Colitis evaluations were made of bloody stool symptoms, hematological date, colon length and microscopic examination. PG improved the symptoms of bloody stools, increase of white blood cells, decrease of red blood cells, shortening of the colon length due to colitis and microscopically promoted healing of mucosal damage induced by DSS.
(Glycotechnological synthesis of proteoglycan)
1. Glycosaminoglycan chains
Reconstruction of Using a transglycosylation activity of testicular hyaluronidase, it was possible to custom synthesize glycosaminoglycan chains.
2. Attachment of GAG chains of protein
Using transglycosylation activity of endo-b-xylosidase, it was possible to transfer of the reconstructed GAG chains to the core protein.
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