| Project/Area Number |
12794010
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Osaka University |
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Principal Investigator |
NOJIMA Hiroshi RIMD, Professor, 微生物病研究所, 教授 (30156195)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAI Katuyuki Cyclex, Co. Ltd., President, 部長(研究職)
OKUZAKI Daisuke RIMD, Research Assistant, 微生物病研究所, 助手 (00346131)
YABUTA Norikazu RIMD, Research Assistant, 微生物病研究所, 助手 (10343245)
TOJI Shingo MBL Co. Ltd., Research Fellow, 研究員
木村 信也 大阪大学, 微生物病研究所, 研究員 (70273703)
藤井 孝之 大阪大学, 微生物病研究所, 助手 (70314416)
吉岡 直寿 大阪大学, 微生物病研究所, 助手 (80314417)
鍋島 建太郎 大阪大学, 微生物病研究所, 助手 (60294120)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥35,200,000 (Direct Cost: ¥35,200,000)
Fiscal Year 2002: ¥17,600,000 (Direct Cost: ¥17,600,000)
Fiscal Year 2001: ¥17,600,000 (Direct Cost: ¥17,600,000)
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| Keywords | quiescent state / growth inhibitory factor / primary cell / transmembrane motif / serum starvation / contact inhibition / cancer cell line / LOH / 初代繊維芽細胞TIG-1 / ヒト初代培養細胞 / サブトラクション / cDNAライブラリー / 癌抑制遺伝子 |
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| Research Abstract |
Mammalian cells stops growth and enter into the static phase or G_0 phase when the concentration of fetal calf serum in the culture medium was reduced from 10% to 0.5%. It was believed that a certain proliferation inhibitory factors exist in the medium harboring the cells in the G_0 phase to maintain the static condition. However, very few studies have been reported on the genes and their gene products that define the G_0 phase. Thus, if a large scale isolation of the genes whose expression was induced is performed, it would be possible to isolate the gene that is required to maintain the static phase by growth-inhibitory function. To isolate such genes comprehensively, we used an improved cDNA library subtraction p as we described previously. Using this method, we have so far been able to isolate more than 60 genes whose expression is induced in growth arrest condition and termed the transcript induced in growth arrest, TIGA, genes. Transcription of some of the TIGA genes are also induced upon contact inhibition of the cell growth. One of the TIGA genes, TIGA1, encodes a small protein with a single transmembrane motif. Ecopically expressed Tiga1 potently inhibited cell growth. Functional analyses of these TIGA genes might shed light to understanding the molecular mechanisms of induction and maintenance of G_0 state of the cell cycle.
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