| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Naoki Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20212871)
HATA Ken-ichro University Hospital, Associate Professor, 医学部附属病院, 助教授 (80293710)
MIZUTANI Hideki National Nagoya Hospital, Medical Chief, 医長 (30167663)
TAKAI Osamu Center for Integranted Research in Science and Engineering, Professor, 教授 (40110712)
岩田 久 名古屋大学, 医学部, 教授 (90023796)
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| Budget Amount *help |
¥37,000,000 (Direct Cost: ¥37,000,000)
Fiscal Year 2002: ¥20,900,000 (Direct Cost: ¥20,900,000)
Fiscal Year 2001: ¥16,100,000 (Direct Cost: ¥16,100,000)
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| Research Abstract |
Recently, it is obvious that mesenchymal stem cells (MSCs) differentiate various human tissues and these cells proliferation technology comes true. On the other hand, biodegradable matrix with differentiation and proliferation of the cells developed by rapid development of matrix engineering. On the basis of these technology, tissue engineering, which involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaffolds and growth factor, was the focus of attention. By using this method, we can regenerate a living tissue using patioents' self. These can cure the difficult treatment of chronic bone and joint disease. At first, we pay attention to develop graft materials as a matrix and improve calcium phosphate. It called CPC and was compared to β-TCP. And in animal study with osteogenesis, it was clear that the materials had good ability of bone regeneration. Therefore we can expect application of the bone materials. And applying fibrin glue and platelet rich plasma, we can confirm that these material have ability of good bone regeneration. As for cell culture method, we can get acceleration of the cell function using stress, such as stretch stress, pressure stress and supersonic waves and apply bone regeneration. There were not big differences in function of chondrocytes, which differentiated from MSCs, using the same stress. And as for culture method on a large scale using a bioreactor, it was useful under using pressure stress. In the future, by applying these results, we would like to suggest cell providing method in regenerating bone and cartilage using MSCs and strategy of fostering new industry.
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