| Project/Area Number |
12794016
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KAMO Naoki Hokkaido Univ. Grad. School of Pharmaceutical Sci., Prof., 大学院・薬学研究科, 教授 (10001976)
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| Co-Investigator(Kenkyū-buntansha) |
AZUMA Hiroshi Hokkaido Red Cross Blood Center, Research Director, 研究部長
IKEDA Hisami Hokkaido Red Cross Blood Center, President, 所長
NARA Toshifumi Hokkaido Univ. Grad. School of Pharmaceutical Sci., Inst., 大学院・薬学研究科, 助手 (30241350)
HIRAYAMA Junichi Hokkaido Red Cross Blood Center, Researcher, 研究部員
ABE Hideki Hokkaido Red Cross Blood Center, Research Section Chief, 研究部係長
阿部 秀樹 北海道赤十字血液センター, 研究部係長
宮内 正二 北海道大学, 大学院・薬学研究科, 助教授 (30202352)
池淵 研二 北海道赤十字血液センター, 研究部長
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥34,500,000 (Direct Cost: ¥34,500,000)
Fiscal Year 2002: ¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 2001: ¥25,700,000 (Direct Cost: ¥25,700,000)
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| Keywords | parvovirus / UV-irradiation / UVC / Heat treatment of virus / S / D treatment / Hemoglobin / CFU / DNA damage / ヒトパルボウイルスB19 / カテキン / メチレンブルー / 活性酸素 / ピリミジンダイマー / 6-4付加体 / 血液凝固第VIII因子 / 一重項酸素 / B19 / 血液凝固第8因子 |
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| Research Abstract |
Blood for transfusion is subject to rigorous inspections for not contaminating virus. However, there are some inevitable possibilities for the contamination because of 1) the necessity of time to produce antibodies against the infected virus (window period) and 2) the existence of unknown virus. Therefore the aim of this research is to develop method of the removal or inactivation of virus in the transfusion blood.
1) We concentrated on parvovirus B19, one of non-enveloped virus which is resistant to the solvent/detergent or heat treatment. In addition, infection of this virus sometimes gives sever illness. First of all, we developed the method testing the infectivity using CFU-E formation. Irradiation of UV-C (750 Jm^<-2>) deceased the infectivity by a factor of 3. The co-presence of rutin or catechins prevents the denaturation of factor VIII without any decrease in the effect against the virus.
2) The UV-C treatment was found to be very useful for the inactivation of virus containing hemoglobin solutions that will be applied to emergency patients who need a lot of blood.
3) We observed a good correlation between the inactivation of virus and the amounts of DNA damage.
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