• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

樹状究起受容野の形成過程における放射状グリアの役割

Research Project

Project/Area Number 12877001
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHokkaido University

Principal Investigator

渡辺 雅彦  北海道大学, 大学院・医学研究科, 教授 (70210945)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Keywords小脳 / プルキンエ細胞 / 樹状突起 / 放射状グリア / バーグマングリア / シナプス形成 / マウス / グルタミン酸受容体
Research Abstract

大脳皮質や小脳皮質では、ニューロンの樹状突起の主軸が軟膜方向へ伸展し、その周囲にシナプスを形成する末梢枝が多数派生するという共通した構築を示す。このような樹状突起構築の指向性がどのような細胞メカニズムにより形成されるのかを検討する目的で、発達段階の小脳に注目して分子形態学的解析を行った。昨年度は、プルキンエ細胞樹状突起をカルビンジン抗体を用いて、バーグマングリアの放射状突起をグルタミン酸トランスポーターGLAST抗体を用いて免疫組織化学を行い、樹状突起がグリア突起と密着して脳表に伸展していく事実を見い出した(Yamada et al.,2000)。本年度は、このグリア突起から樹状突起の伸展作用を有するセリンが分泌されていることを明らかにした。さらに、その分子機構を探索したところ、バーグマングリアには高濃度のセリン合成酵素3PGDHが発現しているが、プルキンエ細胞では全く発現陰性であった。この事実は、セリンを介するニューロングリア間の代謝的カップリングが、樹状突起の伸展誘導機構になっている可能性を示唆する。

Report

(2 results)
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Nakamura, K.: "Enhancement of hippocampal LTP, reference memory and sensorymotor gating in mutant mice lacking a telencephalon-specific cell adhesion molecule"Eur.J.Neurosci.. 13. 179-189 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamazaki, M.: "Differential palmitoylation of two mouse glutamate receptor interacting protein 1 forms with different N-terminal sequences"Neurosci.Lett.. 304. 81-84 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimuta, M.: "Postsynaptic modulation of AMPA receptor-mediated synaptic responses and LTP by the type 3 ryanodine receptor"Mol.Cell Neurosci.. 17. 921-930 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada, K.: "NMDA receptor subunits GluRε1, GluRε3, and GluRζ1 are enriched at the mossy fiber-granule cell synapse in the adult mouse cerebellum"Eur.J.Neurosci.. 13. 2025-2036 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamasaki, M.: "3-phosphoglycerate dehydrogenase (3PGDH), a key enzyme of L-serine biosynthesis, is preferentially expressed in the radial glia/astrocyte lineage and olfactory ensheathing glia in the mouse brain"J.Neurosci.. 21. 7691-7704 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto, K: "Roles of GluRδ2 and mGluR1 in climbing fiber synapse elimination during postnatal cerebellar development"J.Neurosci.. 21. 9701-9712 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada, Keiko: "Dynamic transformation of Bergmann glial fibers proceeds in correlation with dendritic outgrowth and synapse formation of cerebellar Purkinje cells."J.Comp.Neurol.. 418. 106-120 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanaka, Jun: "Gq protein α subunits Gαq and Gαl1 are localized at postsynaptic extra-junctional membrane of cereballar Purkinje cells and hippocampal pyramidal cells."Eur.J.Neurosci.. 12. 781-792 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Minami, T.: "Characterization of nociceptin/orphanin FQ-induced pain responses in conscious mice : Neonatal capsaicin treatment and N-methyl-D-aspartate receptor GluRε subunit knochout mice."Neuroscience. 97. 133-142 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mori, Fumiaki: "Developmental changes in expression of the three ryanodine receptor mRNAs in the mouse brain."Neurosci.Lett.. 285. 57-60 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kakizawa, Sho: "Critical period for activity-dependent synapse elimination in developing cerebellum."J.Neurosci.. 20. 4954-4961 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyazaki, Taisuke: "B2 exon splicing of nonmuscle myosin heavy chain IIB is differently regulated in developing and adult rat brain."Neurosci.Res.. 37. 299-306 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Furuya, Shigeki: "L-serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons."Proc.Natl.Acad.Sci.USA. 97. 11528-11533 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukaya, Masahiro: "Improved immunohistochemical detection of postsynaptically-located PSD95/SAP90 protein family by protease section pretreatment. A study in the adult mouse brain."J.Comp.Neurol.. 426. 572-586 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura, Kazuhiro: "Enhancement of hippocampal LTP, reference memory and sensorymotor gating in mutant mice lacking a telencephalon-specific cell adhesion molecule."Eur.J.Neurosci.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hashimoto, Koichi: "Climbing fiber synapse elimination during postnatal cerebellar development requires signal transduction involving Gαq and phospholipase Cβ4."Progress in Brain Res.. 124. 31-48 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 渡辺雅彦: "脳神経科学イラストレイテッド"森寿,真鍋俊也,渡辺雅彦,岡野栄之,宮川剛(羊土社). 365 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi