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多能性幹細胞の分化過程における染色体の機能的構造変化

Research Project

Project/Area Number 12F02079
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Genetics/Genome dynamics
Research InstitutionKyoto University

Principal Investigator

中辻 憲夫  京都大学, 物質-細胞統合システム拠点, 教授 (80237312)

Co-Investigator(Kenkyū-buntansha) KAFER Georgia  京都大学, 物質-細胞統合システム拠点, 外国人特別研究員
KAFER Georgia  京都大学, 物質―細胞統合システム拠点, 外国人特別研究員
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2014: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2013: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2012: ¥700,000 (Direct Cost: ¥700,000)
KeywordsStem cells / Histones / Acetylation / Chromatin / Trophoblast / Differentiation / Pluripotency / Immunofluorescence / 超解像度顕微鏡 / ヒトES細胞 / ヒストン修飾 / ヒストンバリアント / 細胞分化 / エピジェネティクス / クロマチン
Outline of Annual Research Achievements

My research project investigated the cellular events involved in the differentiation of human embryonic stem (hES) cells into a specialized type of cell called a trophoblast cell. The major part of the project was centered on understanding how chromatin (the architecture of the nucleus) changes when hES cells loose their pluripotency and differentiate into the specialized trophoblast cells. I specifically studied proteins called 'histones' which are the basic unit of chromatin. I was particularly interested in a type of histone protein called 'histone 2A variant Z' (H2AZ). I was interested in H2AZ because it can have different effects on the chromatin architecture when by becoming modified. These modifications primarily concern the addition of small molecules called "acetylation" or "methylation". To study the changes in the modification of H2AZ I used a technique called immunofluorencese (to fluorescently label the proteins or RNA molecules I want to study) I then imaged the cell nucleus with a very sensitive microscope and then perform a specialized type of image processing and analysis that was writen in my laboratory on the captured image. Using these techniques, I found that status of H2AZ acetylation in the stem cell changes very quickly (in as little as 12 hours) following treatment to induce differentiation. I have studied the protein complexes that both acetylate and de-acetylate H2AZ under hES differentiation and found that de-acetylation of H2AZ is critical for differentiation to proceed.

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (2 results)

All 2013

All Presentation (2 results)

  • [Presentation] Peripheral H2AZ. ac marks differentiating hES2013

    • Author(s)
      Georgia R. Kafer
    • Organizer
      The 5th iCeMS Retreat
    • Place of Presentation
      Hikone, Japan
    • Year and Date
      2013-09-06
    • Related Report
      2013 Annual Research Report
  • [Presentation] The distribution of histone variant H2AFZ is restricted according to lineage commitment status in mouse trophohlast cells2013

    • Author(s)
      Georgia R. Kafer ; Peter L. Kaye ; Marie Pantaleon ; Peter M.
    • Organizer
      EMBO Conference series on Chromatin and Epigenetics
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2013-05-09
    • Related Report
      2013 Annual Research Report

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Published: 2013-04-25   Modified: 2024-03-26  

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