マメ科植物における水およびリン利用効率の遺伝的改良

• Project/Area Number: 12F02510
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 外国
• Research Field: 植物分子生物・生理学
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TRAN Lam-Son・P (2013) 独立行政法人理化学研究所, 環境資源科学研究センター, ユニットリーダー; TRAN Lam-Son・P (2012) 独立行政法人理化学研究所, 発現調節研究ユニット, ユニットリーダー
• Co-Investigator(Kenkyū-buntansha): SULIEMAN Saad Abdel Rahman 独立行政法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員 ; SULIEMAN SaadAbdelRahman 独立行政法人理化学研究所, 発現調節研究ユニット, 外国人特別研究員
• Project Period (FY): 2012 – 2014-03-31
• Project Status: Discontinued (Fiscal Year 2013)
• Budget Amount: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2013: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2012: ¥600,000 (Direct Cost: ¥600,000)
• Keywords: N_2 fixation efficiency / Phosphorus deprivation / Crop productivity / Medicago truncatula / Medicago sativa / Chickpea / Carbon and nitrogen metabolisms / N-feedback mechanism / Efficiency / nodule / N_2 fixation / Phosphorus / symbiosis

Research Abstract

We have studied mechanisms of N_2 fixation with the aim to enhance legume crop productivity under phosphonls (P) deficiency. Our findings are as follows :
1. N_2 fixation in Medicago truncatula is mediated through an N-feedback mechanism that is closely related to nodule carbon metabolism under P stress. The critical role of asparagine in the down-regulation of N_2 fixation was revealed.
2. M. truncatula had a higher symbiotic N_2 fixation than M. sativa under P deprivation conditions, which was related to the ability to increase the organic acid concentration in nodules, namely malate.
3. The improved efficiency in chickpea symbiois was associated with the enhanced capacity of organic acid formation and the activities of the key enzymes related to the nodule carbon and nitrogen metabolisms.

Strategy for Future Research Activity

(抄録なし)

Research Products

• [Journal Article] N-feedback regulation is synchronized with nodule carbon alteration in Medicago truneatula under excessive nitrate or low phosphorus conditions (2014)
  • Author(s): Sulieman S, Schulze J, Tran LS
  • Journal Title: J Plant Physiol Volume: (In press) Issue: 3-4 Pages: 290-296
  • DOI: 10.1016/j.physletb.2003.10.071
  • Peer Reviewed
• [Journal Article] Approaches for enhancement of N_2 fixation efficiency of chickpea (Cicer arietinum L.) under limiting nitrogen conditions (2014)
  • Author(s): Esfahani MN, Sulieman S, Schulze J, Tran LS
  • Journal Title: Plant Biotech J Volume: 12(3) Issue: 3 Pages: 387-397
  • DOI: 10.1111/pbi.12146
  • Peer Reviewed
• [Journal Article] The auxin response factor transcription factor family in soybean : genome-wide identifieation and expression analyses during development and water stress (2013)
  • Author(s): Ha CV, Le DT, Nishiyama R, Watanabe Y, Sulieman S, Tran UT, Mochida K, Dong NV, Yamaguchi-Shinozaki K, Shinozaki K, Tran LS
  • Journal Title: DNA Res Volume: 20 Issue: 5 Pages: 511-524
  • DOI: 10.1093/dnares/dst027
  • Peer Reviewed
• [Journal Article] Growth and nodulation of symbiotic Medicago truncatula at different levels of phosphorus availability (2013)
  • Author(s): Sulieman S, Ha CV, Schulze J, Tran LS
  • Journal Title: J Exp Bot Volume: 64(10) Issue: 10 Pages: 2701-2712
  • DOI: 10.1093/jxb/ert122
  • Peer Reviewed
• [Journal Article] Comparative analysis of the symbiotic efficiency of Medicago truncatula and Medicago sativa under phosphorus deficiency (2013)
  • Author(s): Sulieman S, Schulze J, Tran LS
  • Journal Title: Int J Mol Sci Volume: 14(3) Issue: 3 Pages: 5198-5213
  • DOI: 10.3390/ijms14035198
  • Peer Reviewed
• [Book] Databases of transcription factors in legumes, in "Biological Nitrogen Fixation" (2014)
  • Author(s): Mochida K, Ha CV, Sulieman S, Dong NV, Tran LS
  • Publisher: Wiley-Blackwell, John Wiley & Sons, Inc. (In press)