| Project/Area Number |
12J10853
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
General fisheries
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ウォンワランカナ チャニンヤー 東京大学, 大学院農学生命科学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2012 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2013: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2012: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | miRNAs / Takifugu rubripes / genome / piRNAs / メダカ / トラフグ / 次世代シーケンサー / SmallRNAs / ncRNAs |
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| Research Abstract |
1. Purpose ofresearch
Intensive stUdies of miRNAs in many organisms have revealed that the miRNAs were involved in various biological phenomena.
However researches of fish miRNAs have been poorly addressed compare with human and mouse. TorafUgu (Takifugu rubripes), is another model organism withknown genome sequence and one ofthe most intensively studied fish species. The genome of the torafhgu is only about eight times smaller than human genome in size, but has a similar repertoire of protein coding genes.
Therefore, it may also be useful for unveiling non-coding genes including miRNA genes.
2. Research Implementation Plan
We plan to fully identify torafugu miRNA in 9 tissues (Fast and slow muscles, heart, eye, brain, intestine, liver, ovary and testis) in order to understand how specific miRNAs regulate gene expression in the various organs of fish and elucidate their biological function.
3. Result
We found about 9000 known miRNA species, About 2000 miRNA species have not yet been identified in fish but already reported in other species. Moreover, we found about 40 miRNAs species were fish specific. Many known miRNAs showed strong expression in specific tissues. These expression patterns of the known miRNAs were similar with those of previous reports in other animals. Most of miRNAs were mainly expressed in tissues other than ovary and testis. However, some miRNA families were highly abundant in gonads but slightly expressed in somatic tissues, suggesting their specific functions in germ line cell.
Longer small RNAs (26-28 nt) which are abundant in gonads may be putative piRNAs. In addition, the data may also included many putative miRNAs, esiRNAs and a large number of unassigned small RNAs.
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| Strategy for Future Research Activity |
(抄録なし)
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Report
(2 results)
Research Products
(3 results)
