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Molecular Design and Evolution Engineering for Compositc Biochatalysts

Research Project

Project/Area Number 13125101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TORAYA Tetsuo  Okayama Univ., Faculty of Engineering, Professor, 化学研究所, 教授 (50135597)

Co-Investigator(Kenkyū-buntansha) HAYASHI Hideyuki  Osaka Medical College, Faculty of Medicine, Assoc. Prof., 医学部, 助教授 (00183913)
HAYASHI Katsuyuki  Osaka Univ., Institute of Scientific and Industrial Research, Professor, 産業科学研究所, 教授 (20133134)
TORAYA Tetsuo  Okayama Univ., Faculty of Engineering, Professor, 工学部, 教授 (70026318)
SHIMADA Hideo  Keio Univ., School of Medicine, Assoc. Prof., 医学部, 助教授 (80095611)
HIROTSU Ken  Osaka City Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10047269)
堀池 喜八郎  滋賀医科大学, 医学部, 教授 (80089870)
三浦 洌  熊本大学, 医学部, 教授 (70093466)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordscomposite biocatalyst / coenzyme / structure activity relationship / pyridoxal phosphate / cobalamin / flavin / heme / built-in cofactor / キノヘムプロテイン
Research Abstract

Structures and functions of composite biocatalysts were studied, and novel composite biocatalysts were constructed. Toraya et al. established a refined catalytic mechanism for cobalamin-dependent diol dehydratase based on its three-dimensional structure and demonstrated its energetic feasibility by theoretical calculations. Reactivity of the enzyme was modified by protein engineering techniques. Cobalamin composite catalysts were constructed using several apoenzyme models. Tanizawa et al. elucidated the molecular mechanism of biosynthesis and catalytic function of the topaquinone cofactor that is contained in copper amine oxidase and produced from a specific tyrosine residue. Furthermore, a new tryptophan-derived quinone cofactor, cysteine tryptophylquinone, in quinohemoprotein amine dehydrogenase was identified. Miura et al. obtained flavoenzymes with novel reactivity by incorporating artificial flavins to flavoenzyme apoproteins. Horiike et al. revealed molecular bases for substrate specificity of extradiol dioxygenases and 0_2 activation by these enzymes. Hayashi et al. elucidated the refined catalytic reaction mechanisms of pyridoxal enzymes by analyzing the proton-transfer process, thereby presenting the molecular understanding of the "induced fit" and "multisubstrate recognition". Hirotsu et al. determined three-dimensional structures of various natural composite biocatalysts. Esaki et al. revealed the mechanism of biosynthesis of iron-sulfur clusters and developed a novel process for the production of chiral compounds by the use of novel composite biocatalysts. Shimada et al. showed that incorporation of a chemically modified heme into heme proteins is a powerful tool for the preparation of a highly efficient protein-based catalyst. Kitazume et al. demonstrated that catalytic antibodies exhibit a novel function in ionic liquids.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (225 results)

All Other

All Publications (225 results)

  • [Publications] Miyaka, Y., Deshimaru, S., Toraya, T.: "Revised Sequence and Expression of Cyclin B cDNA from the Starfish Asterina pectinifera."Biosci.Biotech.Biochem.. 65. 1119-1126 (2001)

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  • [Publications] Toraya, T., Eda, M., Kamachi, T., Yoshizawa, K.: "Energetic Feasibility of Hydrogen Abstraction and Recombination in coenzyme B12-dependent Diol Dehydratase Reaction."J.Biochem.. 130. 865-872 (2001)

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  • [Publications] Kajiura, H., Mori, K., Tobimatsu, T., Toraya, T.: "Characterization and Mechanism of Action of a Reactivating Factor for Adenosylcobalamin-dependent Glycerol Dehydratase."J.Biol.Chem.. 276. 36514-36519 (2001)

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  • [Publications] 安岡則武, 柴田直樹, 虎谷哲夫: "ラジカル反応の初期過程の構造生物化学:B12補酵素関与ジオールデヒドラターゼの放射光による研究"生物物理. 41(4). 201-204 (2001)

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  • [Publications] 虎谷哲夫: "ラジカルによって触媒される酵素反応:ビタミンB_<12>酵素の立体構造と精密触媒機構"生化学. 74(2). 87-102 (2002)

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  • [Publications] 鏡山博行, 三浦 洌, 虎谷哲夫: "補酵素作用のダイナミズム-酵素の機能を支えるアポタンパクの働き-"ビタミン. 76. 273-282 (2002)

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  • [Publications] Eda, M., Kamachi, T., Yoshizawa, K., Toraya, T.: "Theoretical study on the mechanism of catalysis of coenzyme B12-dependent diol dehydratase."Bull.Chem.Soc.Jap.. 75(7). 1469-1481 (2002)

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  • [Publications] Shibata, N., Masuda, J., Morimoto, Y., Yasuoka, N., Toraya, T.: "Substrate-Induced Conformational Change of a Coenzyme B 12-Dependent Enzyme : Crystal Structure of the Substrate-Free Form of Diol Dehvdratase."Biochemistry. 41(42). 12607-12617 (2002)

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  • [Publications] Yamanishi, M., Tobimatsu, T., Toraya, T.et al.: "The crystal structure of coenzyme B 12-dependent glycerol dehydratase in complex with cobalamin and 1,2-propanediol."Eur.J.Biochem.. 269. 4484-4494 (2002)

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  • [Publications] Fukuoka, M., Yamanishi, M., Zou, X., Brown, L.K., Toraya, T.et al.: "Functions of the D-Ribosyl Moiety and the Lower Axial Ligand of the Nucleotide Loop of Coenzyme B12 in Diol Dehydratase and Ethanolamine Ammonia-lease Reactions"J.Biochem.. 132(6). 935-943 (2002)

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  • [Publications] Toraya, T.: "Enzymatic Radical Catalysis : Coenzyme B12-Dependent Diol Dehydratase."The Chemical Records. 2. 352-366 (2002)

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  • [Publications] Toraya, T.: "Radical Catalysis in Coenzyme B 12- Dependent isomerization (Eliminating) Reactions"Chem.Rev.. 103. 2095-2127 (2003)

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  • [Publications] Shibata, N., Nakanishi, Y., Yamanishi, M., Yasuoka, N.Toraya, T., et al.: "Structural Rationalization for the Lack of Stereospecificity in Coenzyme B12-dependent Dial Dehydratase"J.Biol.Chem.. 278(25). 22717-22725 (2003)

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  • [Publications] 虎谷哲夫: "シンポジウムB群ビタミンはどのように働くか:ビタミンB12酵素の立体構造と精密触媒機構"ビタミン. 77(5・6). 297-312 (2003)

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  • [Publications] Toraya, T., Oka, T., Ando, M., Yamanishi, M., Nishihara, H.: "Novel Pathway for Utilization of Cyclopropanecarboxylate by Rhodococcus rhodochrous."Appl.Environ.Microbiol. 70(1). 224-228 (2004)

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  • [Publications] I.Van denberghe, et al.: "The Covalent Structure of the Small Subunit from Pseudomonas putida Amine Dehydrogenase Reveals the Presence of Three Novel Types of Internal Cross-Linkages, All Involving Cysteine in a Thio-Ether Bond"J.Biol.Chem.. 276. 42923-42931 (2001)

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  • [Publications] S.Datta, et al.: "Structure of a Quinohemoprotein Amine Dehydrogenase with an Uncommon Redox Cofactor and Highly Unusual Crosslinking"Proc.Natl.Acad.Sci.U.S.A.. 98. 14268-14273 (2001)

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  • [Publications] S.Hirota, et al.: "Spectroscopic Observation of Intermediates Formed during the Oxidative Half-Reaction of Copper/Topa Quinone-Containing Phenylethylamine Oxidase"Biochemistry. 40. 15789-15796 (2001)

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  • [Publications] K.Mli_kova, et al.: "Inhibition of Copper Amine Oxidases by Pyridine-Derived Aldoximes and Ketoximes"Biochimie. 83. 995-1002 (2001)

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  • [Publications] C.Ogino, et al.: "Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity"Biochem.Biophys.Acta. 1530. 23-31 (2001)

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  • [Publications] K.Tanizawa, et al.: "UDP-Glucose pyrophosphorylase : primary structure and affinity labeling"J.Biochem.Mol.Biol.Biophys.. 5. 83-98 (2001)

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  • [Publications] 谷澤克行: "バイオファクター研究のブレークスルー:「PQQ」(3)TPQ, TTQ, LTQ"ビタミン. 75. 367-373 (2001)

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  • [Publications] T.Okajima, et al.: "Novel Trimeric Adenylate Kinase from an Extremely Thermoacidophilic Archaeon, Sulfolobus solfataricus : Molecular Cloning, Nucleotide Sequencing, Expression in Escherichia coli, and Characterization of the Recombinant Enzyme"Biosci.Biotech.Biochem.. 66. 2112-2124 (2002)

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  • [Publications] M.Kim, et al.: "X-ray Snapshots of Quinone Cofactor Biogenesis in Bacterial Copper Amine Oxidase"Nature Struct.Biol.. 9. 591-596 (2002)

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  • [Publications] S.Iwaki, et al.: "Real-time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase"Anal.Biochem.. 304. 236-243 (2002)

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  • [Publications] S.Kishishita, et al.: "Role of Copper Ion in Bacterial Copper Amine Oxidase : Spectroscopic and Crystallographic Studies of Metal-substituted Enzymes"J.Am.Chem.Soc.. 125. 1041-1055 (2003)

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  • [Publications] M.Uchida, et al.: "Stereochemistry of 2-Phenylethylamine Oxidation Catalyzed by Bacterial Copper Amine Oxidase"Biosci.Biotechnol.Biochem.. 67. 2664-2667 (2003)

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  • [Publications] Ogino, et al.: "Phospholipase D from Streptoverticillium cinnamoneum : Protein Engineering and Application for Phospholipid Production"J.Mol.Cat.. B23. 107-115 (2003)

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  • [Publications] K.Kataoka, K.Tanizawa: "Alteration of Substrate Specificity of Leucine Dehydrogenase by Site-directed Mutagenesis"J.Mol.Cat.. B23. 299-309 (2003)

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  • [Publications] D.Sun, et al.: "Chemical and Kinetic Reaction Mechanisms of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans"Biochemistry. 42. 10896-10903 (2003)

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  • [Publications] S.Kodera, et al.: "Detection of Protein-Protein Interactions on SiO_2/Si Surfaces by Spectroscopic Ellipsometry"Anal.Biochem.. 321. 65-70 (2003)

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  • [Publications] 谷澤克行: "新しいペプチド・ビルトイン型キノン補酵素:その構造と触媒機能および生合成機構"ビタミン. 77. 275-283 (2003)

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  • [Publications] 岡島俊英, 谷澤克行: "ペプチド・ビルトイン型補酵素研究の新展開.酵素の多彩な触媒機能発現のための"しかけ"を探って"蛋白質核酸酵素. 48. 740-746 (2003)

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  • [Publications] H.Matsunami, et al.: "Chemical Rescue of a Site-specific Mutant of Bacterial Copper Amine Oxidase for Generation of the Topa Quinone Cofactor"Biochemistry. 43. 2178-2187 (2004)

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  • [Publications] R.Miura, C.Setoyama, Y.Nishina, K.Shiga, I.Miyahara, H.Mizutani, K.Hirotsu: "Porcine kidney D-amino acid oxidase : the three-dimensional structure and its catalytic mechanism based on the enzyme-substrate complex model."J.Mol.Catalysis B : Enzymatic. 12. 43-52 (2001)

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  • [Publications] R.Miura: "Versatility and specificity in flavoenzymes : Control mechanisms of flavin reactivity."The Chemical Record. 1. 183-194 (2001)

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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "X-Ray crystallographic study of rat acyl-CoA oxidase II."Protein Sci.. 10. 85-86 (2001)

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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "Crystallization and preliminary X-ray characterization of rat liver acyl-CoA oxidase"Acta Crystallogr.. D57. 1680-1681 (2001)

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  • [Publications] C.Setoyama, Y.Nishina, H.Tamaoki, H.Mizutani, I.Miyahara, K.Hirotsu, K.Shiga, R.Miura: "Effects of hydrogen bonds in association with flavin and substrate in flavoenzyme D-amino acid oxidase. The catalytic and structural roles of Gly313 and Thr317."J.Biochem.. 131. 59-69 (2002)

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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "Three-dimensional structure of the flavoenzyme acyl-CoA oxidase-II from rat liver, the peroxisomal counterpart of mitochondrial acyl-CoA dehydrogenase."J.Biochem.. 131. 365-374 (2002)

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  • [Publications] A.Satoh, Y.Nakajima, I.Miyahara, K.Hirotsu, T.Tanaka, Y.Nishina, K.Shiga, H.Tamaoki, C.Setoyama, R.Miura: "Structure of the transition state analog of medium-chain acyl-COA dehydrogenase. Crystallographic and molecular orbital studies on the charge-transfer complex of medium-chain acyl-CoA dehydrogease with 3-thiaoctanoyl-CoA."J.Biochem.. 134. 297-304 (2003)

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  • [Publications] Y.Nishina, K.Sato, H.Tamaoki, H., Tanaka, T., Setoyama, C., R.Miura, K.Shiva: "Molecular mechanism of the drop in the pKa of a substrate analog bound to medium-chain acyl-CoA dehydrogenase : Implications for substrate activation."J.Biochem.. 134. 835-842 (2003)

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  • [Publications] 三浦洌: "フラビン酵素における反応制御の構造的基盤"ビタミン. 77. 313-320 (2003)

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  • [Publications] Tetsuo Ishida: "Quantitative structure-activity relationship for the cleavage of C3/C4-substituted catechols by a prototypal extradiol catechol Dioxygenases with broad substrate specificity"J.Biochem.. (印刷中). (2004)

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  • [Publications] 石田哲夫: "非ヘム2価鉄イオン単核錯体を利用する酵素群:2-His-1-Carboxylate酵素のO_2を利用した多彩な反応"化学と生物. (印刷中). (2004)

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  • [Publications] Tetsuo Ishida: "Structure and reaction mechanism of catechol 2,3-dioxygenase (metapyrocatechase)"International Congress Series. 1233. 213-220 (2002)

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  • [Publications] Hirofumi Nakajima: "Accurate measurement of near-micromolar oxygen concentrations in aqueous solutions based on enzymatic extradiol cleavage of 4-chlorocatechol : applications to improved low-oxygen experimental systems and quantitative assessment of back diffusion of oxygen from the atmosphere"J.Biochem.. 131. 523-531 (2002)

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  • [Publications] Nakai, Y.: "Yeast Nfs1p is involved in thio-modification of both mitochondrial and cytoplasmic tRNAs."J.Biol.Chem.. 279・13. 12363-12368 (2004)

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  • [Publications] Ikushiro, H.: "Reactions of serine palmitoyltransferase with serine and molecular mechanisms of the actions of serine derivatives as inhibitors."Biochemistry. 43・4. 1082-1092 (2004)

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  • [Publications] Matsuda, N.: "Instability of the apo form of aromatic L-amino Acid decarboxylase in vivo and in vitro : implications for the involvement of the flexible loop that covers the active site."J.Biochem.. 135・1. 33-42 (2004)

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  • [Publications] Hosono: "Glutamine : phenylpyruvate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8."J.Biochem.. 134・6. 843-851 (2003)

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  • [Publications] Islam, M.M.: "Reaction of aspartate aminotransferase with C5-dicarboxylic acids : comparison with the reaction with C4-dicarboxylic acids."J.Biochem.. 134・2. 277-285 (2003)

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  • [Publications] Mizuguchi, H.: "Characterization of histidinol phosphate aminotransferase from Escherichia coli."Biochim.Biophys.Acta. 1647・1-2. 321-324 (2003)

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  • [Publications] Ikushiro, H.: "Bacterial serine palmitoyltransferase : a water-soluble homodimeric prototype of the eukaryotic enzyme."Biochim.Biophys.Acta. 1647・1-2. 116-120 (2003)

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  • [Publications] Hayashi, H.: "Strain and catalysis in aspartate aminotransferase."Biochim.Biophys.Acta. 1647・1-2. 103-109 (2003)

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  • [Publications] Goto, M.: "Crystal structures of branched-chain amino acid aminotransferase complexed with glutamate and glutarate : true reaction intermediate and double substrate recognition of the enzyme."Biochemistry. 42・13. 3725-3733 (2003)

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  • [Publications] Hayashi, H.: "Conformational change in aspartate aminotransferase on substrate binding induces strain in the catalytic group and enhances catalysis."J.Biol.Chem.. 278・11. 9481-9488 (2003)

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  • [Publications] Olmo, M.T.: "Spectroscopic analysis of recombinant rat histidine decarboxylase."J.Biochem.. 132・3. 433-439 (2002)

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  • [Publications] 林秀行: "酵素反応のエネルギー論"生化学. 74・1. 5-16 (2002)

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  • [Publications] Kagamiyama, H.: "Release of enzyme strain during catalysis reduces the activation energy barrier."Chem.Rec.. 1・5. 385-394 (2001)

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  • [Publications] Nakayama, T.: "Specificity analysis and mechanism of aurone synthesis catalyzed by aureusidin synthase, a polyphenol oxidase homolog responsible for flower coloration."FEBS Lea.. 499・1-2. 107-111 (2001)

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      2003 Final Research Report Summary
  • [Publications] Ikushiro, H.: "A water-soluble homodimeric serine palmitoyltransferase from Sphingomonas paucimobilis EY2395T strain, purification, characterization, cloning, and overproduction."J.Biol.Chem.. 276・21. 18249-18256 (2001)

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  • [Publications] H.Mizuguchi, H.Hayashi, K.Okada, I.Miyahara, K.Hirotsu, H.Kagamiyama: "Strain is More Important than Electrostatic Interaction in Controlling the pKa of the Catalytic Group in Aspartate Aminotransferase."Biochemistry. 40(2). 353-360 (2001)

    • Description
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  • [Publications] K.Haruyama, T.Nakai, I.Miyahara, K.Hirotsu, H.Mizuguchi, H.Hayashi, et al.: "Structures of Escherichia coli Histidinol-Phosphate Aminotransferase and Its Complexes with Histidinol-Phosphate and N-(5'-Phosphopyridoxyl)- L-Glutamate : Double Substrate Recognition of the Enzyme"Biochemistry. 40(15). 4633-4644 (2001)

    • Description
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  • [Publications] K.Okada, K.Hirotsu, H.Hayashi, H.Kagamiyama: "Structures of Escherichia coli Branched-Chain Amino Acid Aminotransferase and Its Complexes with 4-Methylvalerate and 2-Methylleucine : Induced Fit and Substrate Recognition of the Enzyme"Biochemistry. 40(25). 7453-7463 (2001)

    • Description
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  • [Publications] T.Tada, C-T.Lu, Y.Nakamura, K.Wada, I.Miyahara, K.Hirotsu, Y.Katsuya, M.Sawada, et al.: "Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme polymethoxy-galactturonase AX1 from Trichosporon penicillatum"Acta Crystallogr D Biol Crystallogr. D57(3). 457-458 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] H.Ura, T.Nakai, S.Kawaguchi, I.Miyahara, K.Hirotsu, S.Kuramitsu: "Substrate recognition mechanism of thermophilic dual-substrate enzyme"J.Biochem.. 130(1). 89-98 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] A.Satoh, J-H.Kim, I.Miyahara, B.Devreese, I.Vandenberghe, A.Hacisalihoglu, et al.: "Crystal Structure of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida"J.Biol.Chem.. 277(4). 2830-2834 (2002)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Goto, Y.Nakajima, K.Hirotsu: "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action"J.Biol.Chem.. 277(18). 15890-15896 (2002)

    • Description
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  • [Publications] R.Omi, H.Mizuguchi, M.Goto, I.Miyahara, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Structure of Imidazole Glycerol Phosphate Synthase from Thermus thermophilus HB8 : Open-Closed Conformational Change and Ammonia Tunneling."J Biochem.. 132(5). 759-765 (2002)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] 佐藤敦子, 広津 建: "最近の研究から「ビルトイン補酵素を持つアミン脱水素酵素の構造研究 - 新規キノン補酵素と異常な分子内架橋-」"日本結晶学会誌. 44. 176-181 (2002)

    • Description
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  • [Publications] M.Goto, Y.Agari, R.Omi, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8"Acta Crystallogr.D Biol.Crystallogr.. 59(2). 356-358 (2003)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Goto, R.Omi, J.Hoseki, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8."Acta Crystallogr D Biol Crystallogr. 59(3). 551-553 (2003)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] A.Satoh, O.Adachi, K.Tanizawa, K.Hirotsu: "The active site structure of quinohemoprotein amine dehydrogenase inhibited by p-nitrophenyl-hydrazine"Biochimica et Biophysica Acta. 1647(1-2). 272-277 (2003)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Goto I.Miyahara H.Hayashi H.Kagamiyama, K.Hirotsu: "Crystal structures of branched-chain amino Acid aminotransferase complexed with glutamate and glutarate : true reaction intermediate and double substrate recognition of the enzyme."Biochemistry. 42(13). 3725-3733 (2003)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Gogo, R.Omi, I.Miyahara, M.Sugahara, K.Hirotsu: "Structures of Argininosuccinate Synthetase in Enzyme-ATP-Substrates and Enzyme-AMP-Product Forms : Stereochemistry of the Catalytic Reaction"J.Biol.Chem.. 278(25). 22964-22971 (2003)

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  • [Publications] R.Omi, M.Goto, I.Miyahara, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal Structures of Threonine Synthase from Thermus thermophilus HB8 ; Conformational change, substrate recognition, and mechanism"J Biol Chem.. 278(46). 46035-46045 (2003)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] 森本幸生: "蛋白質結晶学における二次元検出器の利用"日本結晶学会誌. 45. 289-294 (2003)

    • Description
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  • [Publications] R.Omi, M.Goto, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of histidinol phosphate phosphatase"Acta Crystallogr.D Biol.Crystallogr.. 60(3). 574-576 (2004)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Goto, R.Omi, I.Miyahara, A.Hosono, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal structures of glutamine : phenylpyruvate aminotransferase from Thermus thermophilus HB8: induced fit and substrate recognition"J.Biol.Chem.. 279(16). 16518-16525 (2004)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Goto, R.Omi, N.Nakagawa, I.Miyahara, K.Hirotsu: "Crystal Structures of CTP Synthetase reveal ATP, UTP, and Glutamine Binding Sites"Structure. 12(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] A.Watanabe et al.: "Stereochemistry of hydrogen abstraction from pyridoxamine phosphate catalyzed by alanine racemase of Bacillus stearothermophilus"J.Mol.Catal.B.Enzymatic. 12. 145-150 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] T.Uo et al.: "Purification and characterization of alanine racemase from hepatopancreas of black-tiger prawn, Penaeus monodon"J.Mol.Catal.B : Enzymatic. 12. 137-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] T.Uo et al.: "Functional characterization of alanine racemase from Schizosaccharomyces pombe : a eucaryotic counterpart to bacterial alanine racemase"J.Bacteriol.. 183. 2226-2233 (2001)

    • Description
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  • [Publications] T.Suzuki et al.: "Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain no.6"J.Biosci.Bioeng.. 92. 144-148 (2001)

    • Description
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  • [Publications] K.Soda et al.: "Stereospecificity for the hydrogen transfer of pyridoxal enzyme reactions"The Chemical Record. 1. 373-384 (2001)

    • Description
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  • [Publications] S.Kato et al.: "Screening and identification of a protein interacting with IscU"微量栄養素研究. 18. 105-109 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] M.Kashiwa et al.: "Removal of soluble selenium by a selenate-reducing bacterium Bacillus sp. SF-1"BioFactors. 14. 261-265 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] H.Mihara et al.: "Role of cysteine desulfurase in the biosynthesis of formate dehydrogenase H containing Mo, molybdopterin, selenocysteine, and Fe_4S_4 cluster"Biomed.Res.Trace.Elem.. 12. 261-262 (2001)

    • Description
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  • [Publications] A.Lewandowicz et al.: "Chlorine kinetic isotope effect on fluoroacetate dehalogenase reaction"J.Am.Chem.Soc.. 123. 9192-9193 (2001)

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  • [Publications] K.Yokoigawa et al.: "Structure and function of psychrophilic alanine racemase"J.Mot.Catal.B : enzymatic. 12. 27-35 (2001)

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  • [Publications] A.Watanabe et al.: "Reaction mechanism of alanine racemase from Bacillus stearothermophilus : X-ray crystallographic studies of the enzyme bound with N-(5'-phosphopyridoxyl)-alanine"J.Biol.Chem.. 277. 19166-19172 (2002)

    • Description
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  • [Publications] T.Uo et al.: "Gene cloning, purification and characterization of 2,3-diaminopropionate ammonia-lyase from Escherichia col"Biosci.Biotechnol.Biochem.. 66. 2639-2644 (2002)

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  • [Publications] U.Tokumoto et al.: "Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Eschrichia col"J.Biochem.. 131. 713-719 (2002)

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  • [Publications] T.Suzuki et al.: "A cold-active esterase with a substrate preference for vinyl esters from a psychrotroph, Acinetobacter sp.strain no.6 : gene cloning, purification, and characterization"J.Mol.Catal.B : Enzymatic. 16. 255-263 (2002)

    • Description
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  • [Publications] T.Suzuki et al.: "Primary structure and catalytic properties of a cold-active esterase from a psychrotroph, Acinetobacter sp.strain no.6 isolated from Siberian soil"Biosci.Biotechnol.Biochem.. 66. 1682-1690 (2002)

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  • [Publications] S.Kato et al.: "Cys328 of IscS and Cys63 of IscU are the sites of disulfide bridge formation in a covalently-bound IscU/IscS complex : Implications for the mechanism of iron-sulfur cluster assembly"Proc.Natl.Acad.Sci.. 99. 5948-5952 (2002)

    • Description
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  • [Publications] E.Pilon-Smits et al.: "Characterization of a NifS-like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism"Plant Physiol.. 130. 1309-1318 (2002)

    • Description
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  • [Publications] H.Bae et al.: "Application of a thermostable glutamate racemase from Bacillus sp. SK-1 for the production of D-phenylalanine in a multi-enzyme system"J Mol Catal B : Enzymatic. 17. 223-233 (2002)

    • Description
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  • [Publications] 山西 守, 飛松孝正, 虎谷哲夫: "生物工学実験書 改訂版 (日本生物工学会編)"培風館. 480 (2002)

    • Description
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  • [Publications] 虎谷哲夫: "ビタミン研究のブレークスルー-発見から最新の研究まで- (日本ビタミン学会)"学振出版. 296 (2002)

    • Description
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  • [Publications] 虎谷哲夫: "廣川タンパク質化学 第4巻 酵素 (福井哲也・伊藤正樹編集)"廣川書店. 366 (2003)

    • Description
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  • [Publications] 後藤祐児, 谷澤克行(編著): "バイオサイエンスの新世紀3 タンパク質の分子設計"共立出版株式会社. 201 (2001)

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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "Agency for Scientific Publ. Berlin (Eds. Chapman, S., Perham, R., Scrutton, N.)"Flavins and Flavoproteins. 6 (2002)

    • Description
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  • [Publications] 江崎信芳 他: "生化学基礎の基礎"化学同人. 326 (2002)

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  • [Publications] N.Esaki et al.: "Isomerizations. Enzyme Catalysis in Organic Synthesis second edition (Edited by K Drauz and H.Waldmann)"VCH. 53 (2002)

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  • [Publications] S.P.Manandhar et al.: "Oxygen and Life - Oxygenases, Oxidases and Lipid Mediators"Elsevier Science Amsterdam, Boston, New York, Oxford, Paris, Sandiego, San Francisco, Singapore, Tokyo. 560 (2002)

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  • [Publications] 下方国稔 他: "ゲノム・プロテオミクスの新展開-生物情報の解析と応用"エヌアイエス出版、東京. 1158 (2004)

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  • [Publications] T., Toraya, M.Eda, T.Kamachi, K.Yoshizawa: "Energetic feasibility of hydrogen abstraction and recombination in coenzyme B_<12>-dependent diol dehydratase dreaction."J. Biochem.. 130. 865-872 (2001)

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  • [Publications] H.Kjiura, K.Mori, T.Tobimatsu, T.Toraya: "Characterization and mechanism of action of reactivation factor for adensylcobalamin-dependent glycerol dehydratase."J. Biol. Chem.. 276. 36514-36519 (2001)

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  • [Publications] J.Masuda, N.Shibata, Y.Morimoto, T.Toraya, N.Yasuoka: "Radical production simulated by photo-irradiation of the diol dehydratase-adeninylpentylcobalamin complex."J. Synchrotron Radiat.. 8. 1182-1185 (2001)

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  • [Publications] Y.Miyaka, S.Deshimaru, T.Toraya: "Revised sequence and expression of cyclin B cDNA from the starfish Asterina pectinifera."Biosci. Biotech. Biochem.. 65(5). 1119-1116 (2001)

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  • [Publications] M.Eda, T.Kamachi, K.Yoshizawa, T.Toraya: "Theoretical study on the mechanism of catalysis of conezyme B12-dependent diol dehydratase."Bull. Chem. Soc. Jap.. 75(7). 1469-1481 (2002)

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  • [Publications] N.Shibata, J.Masuda, Y.Morimoto, N.Yasuoka, T.Toraya: "Substrate-induced conformational change of cenzyme B12-dependent enzyme : Crystal structure of the subsrate-free form of diol dehydratase."Biochemistry. 41(42). 12607-12617 (2002)

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  • [Publications] M.Yamanishi, M.Yunoki, T.Tobimatsu, H.Sato, J.Matsui A.Dokiya, Y.Iuchi, K.Oe, K.Suto, N.Shibara, Y.Morimoto, N.Yasuoka, T.Toraya: "The crystal structure of coenzyme B12-ependent glycerol dehydratase in complex with cobalamin and 1, 2-propanediol."Eur. J. Biochem.. 269. 4484-4494 (2002)

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  • [Publications] M.Fukuoka, S.Yamada, S.Miyoshi, K.Yamashita, M.Yamashi, X.Zou, L.K.Brown, T.Toraya: "Functions of the D-ribosyl moiety and teh lower axial ligand of the nucleotide loop of conezyme B12 in diol dehydratase and ethanolamine ammonia-lyase reactions."J. Biochem.. 132(6). 935-943 (2002)

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  • [Publications] T.Toraya: "Enzymatic radical catalysis : Coenzyme B12-dependent diol dehydratase."The Chemical Records. 2. 352-366 (2002)

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  • [Publications] T.Toraya: "Radical catalysis in coenzyme B12-dependent isomerization (elininating) reactions."Chem. Rev.. 103. 2095-2127 (2003)

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  • [Publications] N.Shibata, Y.Nakanishi, M.Fukuoka, M.Yamanishi, N.Yasuoka, T.Toraya: "Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase."J. Biool. Chem.. 278(25). 22717-22725 (2003)

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  • [Publications] T.Toraya, T.Oka, M.Ando, M.Yamanishi, H.Nishihara: "Novel pathway for utilization of cyclopropanecarboxylate by Rhodococcus rhodochrous."Appl. Environ. Micobiol.. 70(1). 224-228 (2004)

    • Description
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  • [Publications] I.Vandenberghe, J.-K.Kim, B.Devreese, A.Hacisalihoglu, H.Iwabuki, T.Okajima, S.Kuroda, O.Adachi, J.A.Jongejan, J.A.Duine, K.Tanizawa, J.Van Bdeeumen: "The covalent structure of the small subunit from Pseudomonas putida amine dehydrogenase reveals the presence of three novel types of internal cross-linkages, all involving cysteine in a thio-ether bond."J. Biol. Chem.. 276. 42923-42931 (2001)

    • Description
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  • [Publications] S.Datta, Y.Mori, K.Takagi, K.Kawaguchi, Z.W.Chen, T.Okajima, S.Kuroda, T.Ikeda, K.Kano, K.Tanizawa, F.S.Mathews: "Structure of a quinohemoprotein amine dehydrogenase with an uncommon redox cofactor and highly unusual crosslinking."Proc. Natl. Acad. Sci. U.S.A.. 98. 14268-14273 (2001)

    • Description
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  • [Publications] S.Hirota, T.Iwamoto, S.Kishishita, T.Okajima.O.Yamauchi, K.Tanizawa: "Spectrosocpic observation of intermediates formed during the oxidative half-reaction of coppertopa quinone-containing phenylethylamine oxidase."Biochemistry. 40. 15789-15796 (2001)

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  • [Publications] K.Mlickova, M.Sebela, R.Cibulka, L.Frebort, P.Pec, F.Liska, K.Tanizawa: "Inhibition of copper amine oxidases by pyridine-derived aldoximes and ketoximes."Biochimie. 83. 995-1002 (2001)

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  • [Publications] C.Ogino, Y.Negi, H.Daido, M.Kanemasu, A.Kondo, S.Kuroda, K.Tanizawa, N.Shimizu, H.Fukuda: "Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity."Biochem. Biophys. Acta. 1530. 23-31 (2001)

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  • [Publications] K.Tanizawa, M.Kusunoki, T.Fukui: "UDP-glucose pyrophosphorylase : primary structure and affinity albeling."J. Biochem. Mol. Biol. & Biophys.. 5. 83-98 (2001)

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  • [Publications] T.Okajima, D.Kitaguchi, K.Fujii, H.Matsuoka, S.Goto, S.Uchiyama, Y.Kobayashi, K.Tanizawa: "Novel trimeric adenylate kinase from an extremely thermoacidophilic archaeon, Sulfolobus solfataricus : molecular cloning, nucleotide sequencing, expression in Escherichia coli, and characterization of the recombiant enzyme."Biosci. Biotech. Biochem.. 66. 2112-2124 (2002)

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  • [Publications] M.Kim.T.Okajima, S.Kishishita, M.Yoshimura, A.Kawamori, K.Tanizawa, H.Yamaguchi: "X-tay snapshots of quinone cofactor biogenesis in bacterial copper amine oxidase."Nature Struct. Biol.. 9. 591-596 (2002)

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  • [Publications] S.Iwaki, M.Ogasawar, R.Kurita, O.Niwa, K.Tanizawa, Y.Ohashi, K.Mdaeyama: "Real-time monitoring of histamine released from rat basophilic leukemia (RBL-2H3) cell with a histamine microsensor using recombinant histamine oxidase."Anal. Biochem.. 304. 236-243 (2002)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] S.Kishishita, T.Okajima, M.Kim H.Yamaguchi, S.Hirota, S.Suzuki, S.Kuroda, K.Tanizawa, M.Mure: "Role of copper ion in bacterinal copper amine oxidase : spectroscopic and crystallographic studies of metal-substituted enzymes."J. Am. Chem. Soc.. 125. 1014-1055 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Uchida, A.Ohtani, N.Kohyama, T.Okajima, K.Tanizawa, Y.Yamamoto: "Stereochemistry of 2-phenylethylamine oxidation catalyzed by bacterial copper amine oxidase."Biosic, Biotechnol. Biochem.. 67. 2664-2667 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] C.Ogino, S.Kuroda, S.Tokuyama, A.Kondo, N.Shimizu, K.Tanizawa, H.Fukuda: "phospholipase D from Streptoverticillium cinnamoneum : protein engineering and application for phospholipid production."J. Mol. Cat.. B23. 107-115 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] K.Kataoka, K.Tanizawa: "Alteration of substrate specificity of leucine dehydrogenase by site-directed mutagenesis."J. Mol. Cat.. B23. 299-309 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] D.Sun, K.Ono, T.Okajima, K.Tanizawa, M.Uchida, Y.Yamamoto, F.S.Mathews, V.L.Davidason: "Chemical and kinetic reaction mechanisms of quinohemoprotein amine dehydrogenase fro mParacocus denitrificans."Biochemistry. 42. 10896-10903 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] S.Kodera, T.Okajima, H.Iwabuki, D.Kitaguchi, S.Kuroda, T.Yoshinobu, K.Tanizawa, M.Futai, H.Iwasaki: "Detection of protein-protein interactions on Siol_2/Si surfaces by spectroscopic ellipsometry."Anal. Biochem.. 321. 65-70 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Matsunami, T.Okajima, S.Hirota, H.Yamaguchi, H.Hori, S.Kuroda, K.Tanizawa: "Chemical rescue of a site-specific mutant of bacterial copper amine oxidase for gencration of the topa quinane cofactor."Biochemistry. 43. 2178-2187 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] R.Miura, C.Setoyama, Y.Nishina, K.Shiga, I.Miyahara, H.Mizutani, K.Hirotsu: "Porcine kidney D-amino acid oxidase : the three-dimensional structure and its catalytic mechanism based on the enzyme-substrate complex model."J. Mol. Catalysis B : Enzymatic. 12. 43-52 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] R.Miura: "Versatility and specificity in flavoenzymes : Control mechanisms of flavin reactivity."The Chemical Record. 1. 183-194 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, Setoyama, H.Tamaoki, R.Miura: "X-Ray crystallographic study of rat acyl-CoA oxidase II."Protein Sci.. 10, Suppl.2. 85-86 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "Crystallization and preliinary X-ray characterization of rat live acyl-CoA oxidase."Acta Crystallogr.. D57. 1680-1681 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Y.Nishina, K.Sato, R.Shi, C.Setoyama, R.Miura, K.Shiga: "On theligands in charge-transfer complexes of porcine kidney flavoenzyme D-amino acid oxiase in three redox states : A resonance Raman study."J. Biochem.. 130. 637-647 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] C.Setoyama, Y.Nishina, H.Tamaoki, H.Mizutani, I.Miyahara, K.Horitsu, K.Shhiga, R.Miura: "Effects of hydrogen bonds in association with flavin and substrate in flavoenzyme D-amino acid oxidase. The catalytic and structural roles of Gly313 and Thr 317"J. Biochem.. 131. 59-69 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Y.Nakajima, I.Miyahara, K.Hirotsu, Y.Nishina, K.Shiga, C.Setoyama, H.Tamaoki, R.Miura: "Three-deimensional structure of the flavenzyme acyl-CoA oxidase-II from rat liver, the peroxisomal counterpart of mitochondrial acyl-CoA dehydrogenase."J. Biochem.. 131. 365-374 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] A.Satoh, Y.Nakajima, I.Miyahara, K.Hirotsu, T.Tanaka, Y.Nishina, K.Shiga, H.Tamaoki, C.Setoyama, R.Miura: "Structure of the transition state analog of medifum-chain acyl-CoA dehydrogenase. Crystallographic and molecular orbital studies on the charge-transfer complex of medium-chain acyl-CoA dehydrogenase with 3-thiaoctanoyl-CoA."J. Biochem.. 134. 297-304 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Y.Nishina, K.Sato, H.Tamaoki, H., Tanaka, T., Setoyama, C., R.Miura, K.Shiga: "Molecular mechanism of the drop in the pKa of a substrate analog bound to medium-chain acyl-CoA dehydrogenase : Implications for substrate activation."J. Biochem.. 134. 835-842 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Nakajima, T.Ishida, H.Tanaka, K.Horiike: "Accurate measurement of near-micromolar oxygen concentrations in aqueous solutions based on enzymatic extradiol cleavage of 4-chlorocatechol : applications to improved low-oxygen experimental systems and quantitative assessment of back diffusion of oxygen from the atmosphere."J. Biochem.. 131. 523-531 (2002)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Ishida, A.Kita, K.Miki, M.Nozaki, K.Horiike: "Structure and reaction mechanism of catechol 2,3-dioxygenase (metapyrocatechase)."International Congress Series. 1233. 213-220 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Ishida, H.Tanaka, K.Horiike: "Quantitative structure-activity relationship for the cleaveage of C3/C4-substituted catecholsy by a prototypal extradiol catechol dioxygenases with broad substrate specificity."J. Biochem.. (in press). (2004)

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  • [Publications] H.Ikushiro, H.Hayashi, H.Kagamiyama: "A water-soluble homodimeric serine palmitoryltransferase from Sphingomonas paucimobilis EY2395T strain. purification, characterization, cloning, and overproduction."J. Biol. Chem.. 276. 18249-18256 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Nakayama, T.Sat.Y.Fukui, K.Yonekura-Sakakibara, H.Hayashi, Y.Tanaka, T.Kusumi, T.Nishino: "Sdepcificity analysis and mechanism of aurone synthesis actalyzed by aureusidin synthase, a polyphenol oxidase homolog responsible for flower coloration."FEBS Lett.. 499. 107-111 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Kagamiyama, H.Hayashi: "Release of enzyme strain during catalysis reduces the activation energy barrier."Chem. Rec.. 1. 385-394 (2001)

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  • [Publications] M.T.Olmo, F.Sanchez-Jimenez, M.A.Medina, H.Hayashi: "Spectroscopic analysis of recombinant rat histidine decarboxylase."J. Biochem.. 132. 433-439 (2002)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Hayashi, H.Mizuguchi, I.Miyahara, Y.Nakajima, K.Hirotsu, H.Kagamiyama: "Conformational change in aspartate aminotransferase on substrate binding induces strain in te catalytic group and enhances catalysis."J. Biol. Chem.. 278. 9481-9488 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Hayashi, H.Mizuguchi, I.Miyahara, M.M.Islam, H.Ikushiro, Y.Nakiajima, K.Hirotsu, H.Kagamiyama: "Strain and catalysis in asparate aminotransferase."Biochim. Biophys. Acta. 1674. 103-109 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Ikushiro, H.Hayashi, H.Kagamiyama: "Bacterial serine plamitoyltransferase : a water-soluble homodimeric prototype of the eukaryotic enzyme."Biochim. Biophys. Acta. 1647. 116-120 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Mizuguchi, H.Hayashi, I.Miyahara, K.Hirotsu, H.Kagamiyama: "Characterization of histidinol phosphate aminotransferase from Escherichia coli."Biochim. Biophys. Acta. 1647. 321-324 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.M.Islan, H.Hayashi, H.Kagamiyama: "Reation of aspartate ainotransferase with C5-dicarboxylic acids : comparison with the reaction with C4-dicarboxylic acids."J. Biochem.. 134. 277-285 (2003)

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  • [Publications] A.Hosono, H.Mizuguchi, H.Hayashi, M.Goto, I.Miyahara, K.Hirotsu, H.Kagamiyama: "Glutamine : phenylpyruvate aminotransferase from an extremely thermophilic bacterium, Thermus themophilus HB8."J. Biochem.. 134. 843-851 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] N.Matsuda, H.Hayashi, S.Miyatake, T.Kuroiwa, H.Kagamiyama: "Instability of the apo form of aromatic L-amino acid decarboxylase in vivo and in vitro : implications of the involement of the flexible loop that covers the active site."J. Biochem.. 135. 33-42 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Ikushiro, H.Hayashi, H.Kagamiyama: "Rections of serine plamitoyltransferase with serine and molecular mechanisms of the actions of serine derivatives as inhibitors."Biochemistry. 43. 1082-1092 (2004)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] Y.Nakai, N.Umeda, T.Suzuki, M.Nakai, H.Hayashi, K.Watanabe, H.Kagamiyama: "Yeast Nfs1P is inveolved in thio-modification of both mitochondrial and cytoplasmic cRNAs."J. Biol. Chem.. 279. 12363-12368 (2004)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Mizuguchi, H.Hayashi, K.Okada, I.Miyahara, K.Hirotsu, H.Kagamiyama: "Strai si more important than electrostatic interaction in controlling the pKa of the catalytic group in asparate aminotransferase."Biochemistry. 40. 353-360 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] K.Haruyama, T.Nakai, I.Miyahara, K.Hirotsu, H.Mizuguchi, H.Hayashi, H.Kagamiyama: "Structures of Escherichia coli histidinol-phosphate aminotransferase and its complexes with histidinol-phosphate and N-(5'-phosphopyridoxyl)-L-glutamate : Double substrate recognition of the enzyme."Biochemistry. 40. 4633-4644 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] K.Okada, K.Hirotsu, H.Hayashi, H.Kagamiyama: "Structures of Escherichia coli branched-chain amino acid aminotransferase adn tis complexes with 4-methylvaterate and 2-methylleucine : Induced fit and substrae recognion of the enzyme."Biochemistry. 40. 7453-7463 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Tada, C-T.Lu, Y.Nakamura, K.Wada, I.Miyahara, K.Hirotsu, Y.Katsuya, M.Sawada, M.Takao, T.Sakai, K.Nishimura: "Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme polymethoxy-galactturonase AX1 fro Trichosporon penicillatum."Acta Cryst.. D57. 457-458 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Ura, T.Nakai, S.Kawaguchi, I.Miyahara, K.Hirotsu, S.Kurumitsu: "Substrate recognition mechanism of thermophilic dual-substrate enzyme."J. Biochem.. 130. 89-98 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] A.Satoh, J-H.Kim.I.Miyahara, B.Devreese, I.Vandenberghe, A.Hacisalihoglu, T.Okajima, S.Kuroda, O.Adachi, J.A.Duine, J.Van Beeumen, K.Tanizawa, K.Hirotsu: "Crystal structure of quinohemoprotein amine dehydrogenase from Pseudomanas putida"J. Biol. Chem.. 277. 2830-2834 (2002)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto, Y.Nakajima, K.Hirotsu: "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action."H. Biol. Chem.. 277. 15890-15896 (2002)

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  • [Publications] R.Omi, H.Mizuguchi, M.Goto, I.Miyahara, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8 : Open-closed conformation change and annomia tunneling"J. Biochem.. 132. 759-765 (2002)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto, Y.Agari, R.Omi, I.Miyahara, K.Hirotsu: "Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8. Acta Crystallogr."D Biol. Crystallogr.. 59. 356-358 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto, R.Omi, J.Hoseki, N.Nakagawa, I.Miyahara, K Hirotsu: "Expression, purification and preliminary Xray characterization of CTP synthetase from Thermus thermophilus HB8."Acta Crystallogr D Biol Crystallogr.. 59. 551-553 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] A.Satoh, O.Adachi, K.Tanizawa, K.Hirotsu: "The active site structure of quinohemoprotein amine dehydrogenase inhibited by p-nitrophenyl-hydrazine."Biochimica et Biophysica Acta. 1647. 272-277 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto I.Miyahara H.Hayashi H.Kagamiyama, H.Hirotsu: "Crystal structures of branched-chai amino acid aminotransferase complexed with glutamate and glutarate : true reaction intermediate and double substrae recognition of the enzyme."Biochemistry. 42. 3725-3733 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto, R.Omi, I.Miyahara, M.Sughara, K.Hitosu: "Structures of argininosuccinate synthetase in enzyme-ATP-substrates and enzyme-AMP-product forms : Stereochemistry of the catalytic reaction."J. Biol. Chem.. 278. 22964-22971 (2003)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] R.Omi, M.Goto, I.Miyahara, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal Structures of threonine synthease from Thermus thermophilus HB8 : Conformation change, substrate recognition, and mechan."J. Biol. Chem.. 278. 46035-46045 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] R.Omi, M.Goto, N.Nakagawa, I.Miyahara, K.Hirotsu: "Expression, puritication and pretiminary X-ray characterization of histidinol phosphate phosphatase. Acta Crystallogr."D Biol. Crystallogr.. 60. 574-576 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto, R.Omi, I.Miyahara A.Hosono, H.Mizuguchi, H.Hayashi, H.Kagamiyama, K.Hirotsu: "Crystal strutures of glutamine : phenylphruvate aminotransferase from Thermus thermophilus HB8 : induced fit and substrate recognition."J. Biol. Chem.. 279. 16518-16525 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Goto, R.Omi, N.Nakagaw, I.Miyahara, K.Hirotsu: "Crystal structures of CTP synthetase eveal ATP, UTP, and glutamine binding sites."Structure. 12(in press). (2004)

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  • [Publications] M.Sato, N.Shibata, Y.Morimoto, Y.Takayama, K.Ozawa, H.Akutsu, Y.Higuchi, N.Yasuoka: "X-ray induced reduction of the crystal of high-moleucular weight cytochrome c revealed by miroscopectrophotometry."J. Synchrotron Rad.. 11. 113-116 (2004)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] A.Watanabe, T.Yoshimura, Y.H.Lim.Y.Kurokawa, K.Soda, N.Esaki: "Stereochemistry of hydrogen abstracton from pyridoxamine phosphate catalyzed by alanine racemase of Bacillus stearothemophilus."J. Mol. Catal. B : Enzymatic. 12. 145-150 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Uo, T.Ueda, T.Nishiyama, T.Yoshimura, N.Esaki: "Purification and characterization of alanine racemase from hepatopancreas of black-tiger prawn, Penaeus monodon"J. Mol. Catal. B : Enzymatic. 12. 137-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Uo, T.Yoshimura, N.Tanaka, K.Takegawa, N.Esai: "Functional characterizaton of alanine racemase from Schizosacharomyces pombe : a eucaryotic counterpart to bacterial alanine racemase."J. Bacteriol.. 183. 226-2233 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] T.Suzuki, T.Nakayama, T.Kurihara, T.Nishino, N.Esaki: "Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain no. 6."J. Biosci. Bioeng.. 92. 144-148 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] K.Soda, T.Yoshimura, N.Bsaki: "Stereospecificity for the hydrogen transfer of pyridoxal enzyme reactions."The Chemical Record. 1. 373-384 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] M.Kashiwa, M.Ike, H.Mihara, N.Esaki, M.Fujita: "Removal of soluble selenium by a selenate-reducing bacterium Bacillus sp."SF-1. BioFactors. 14. 261-265 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] H.Mihara, S.Kato, T.Kurihara, T.Yoshimura, N.Esaki, G.M.Lacourciere, T.C.Stadtman: "Role of cysteine desulfurase in the biosynthesis of formate dehydrogenase H containing Mo, molybdopterin, selenocysteine, and Fe_4S_4 cluster."Biomed. Res. Trace. Elem.. 12. 261-262 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] A.Lewandowicz, D.Sicinska, J.Rudzinski, S.Ichiyama, T.Kurihara, N.Esaki, P.Paneth: "Chlorine kinetic isotope effect on fluoroacetate dehalogenase reaction"J. Am. Chem. Soc.. 123. 9192-9193 (2001)

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      「研究成果報告書概要(欧文)」より
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  • [Publications] K.Yokoigawa, Y.Okubo, H.Kawai, N.Esaki, K.Soda: "Structure and function of psychrophilic alanine racemas"J. Mol. Catal. B : Enzymatic. 12. 27-35 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] A.Watanabe, T.Yoshimura, B.Mikami, H.Hayashi, H.Kagamiyama, N.Esaki: "Reaction mechanism of alanine racemase from Bacillus stearothermophilus : X-ray crystallographic studies of the enzyme bound with N-(5'-phosphopyridoxyl)-alanine"J. Biol. Chem.. 277. 19166-19172 (2002)

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  • [Publications] T.Uo, T.Yoshimura, T.Nishiyama, N.Esaki: "Gene cloning, purification and characterization of 2,3-diaminopropionate ammonia-lyase from Escherichia coli."Biosci. Biotechnol. Biochem.. 66. 2639-2644 (2002)

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  • [Publications] U.Tokumoto, S.Nomura, Y.Minami, H.Mihara, S.Kato, T.Kurihara, N.Esaki, H.Kanazawa, H.Matsubara, Y.Takahashi: "Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Eschrichia coli."J. Biochem.. 131. 713-719 (2002)

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  • [Publications] T.Suzuki, T.Nakayama, T.Kurihara, T.Nishino, N.Esaki: "A cold-active esterase with a substrate preference for vinyl esters from a psychrotroph, Acinetobacter sp. strain no.6 : gene cloning, purification, and characterization"J. Mol. Catal. B : Enzymatic. 16. 255-263 (2002)

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  • [Publications] T.Suzuki, T.Nakayama, T.Kurihara, T.Nishino, N.Esaki: "Primary structure and catalytic properties of a cold-active esterase from a psychrotroph, Acinetobacter sp. strain no.6 isolate from Siberian soil"Biosci. Biotechnol. Biochem.. 66. 1682-1690 (2002)

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  • [Publications] S.Kato, H.Mihara, T.Kurihara, Y.Takahashi, U.Tokumoto, T.Yoshimura, N.Esaki: "Cys328 of IscS and Cys63 of IscU are the sites of disulfide bridge formation in a covalently-bound IscU/IscS complex : Implications for the mechanism of iron-sulfur cluster assembly"Proc. Natl. Acad. Sci.. 99. 5948-5952 (2002)

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      「研究成果報告書概要(欧文)」より
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      2003 Final Research Report Summary
  • [Publications] E.Pilon-Smits, G.Garifullina, S.bdel-Ghany, S.Kato, H.Mihara, K.Hale, J.Burkhead, N.Esaki, T.Kurihara, M.Pilon: "Characterization of a NifS-like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism"Plant Physiol.. 130. 1309-1318 (2002)

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  • [Publications] H.Bae, S.Hong, S.Lee, M.Kwak, N.Esaki, M.Sung: "Application of a thermostable glutamate racemase from Bacillus sp. SK-1 for the production of D-phenylalanine in a multi-enzyme system"J Mol Catal B : Enzymatic. 17. 223-233 (2002)

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  • [Publications] S.Ichiyama, T.Kurihara, M.Miyagi, A.Galkin, S.Tsunasawa, H.Kawasaki, N.Esaki: "Catalysis-linked inactivation fluoroacetate dehalogenase by ammonia : A novel approach to the active-site environment"J. Biochem.. 131. 671-677 (2002)

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  • [Publications] T.A.Muranova, S.N.Ruzheinikov, S.E.Sedelnikova, P.J.Baker, A.Pasquo, A.Galkin, N.Esaki, T.Ohshima, K.Soda, D.W.Rice: "Crystallization and preliminary X-ray analysis of substrate compleses of leucine dehydrogenase from Thermoactinomyces intermedius"Acta Cryst.. D58. 1059-1062 (2002)

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  • [Publications] H.Mihara, N.Esaki: "Bacterial cysteine desulfurases : their function and mechanisms"Appl. Microbiol. Biotechnol. 60. 12-23 (2002)

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  • [Publications] H.Mihara, N.Esaki: "Selenocysteine lyase from mouse liver"Methods in Enzymology. 347. 198-203 (2002)

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  • [Publications] H.Mihara, S.Kato, G.M.Lacourciere, T.C.Standman, R.A.J.D.Kennedy, T.Kurihara, U.Tokumoto, Y.Takahashi, N.Esaki: "The iscS gene is essential for the biosynthesis of 2-selenouridine in tRNA and the selenocysteine containing fromate dehydrogenase"H. Proc. Natl. Acad. Sci.. 99. 6679-6683 (2002)

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  • [Publications] H.Mihara, T.Fujii, S.Kato, T.Kurihara, Y.Hata, N.Esaki: "Structure of external aldimine of Escherichia coli CsdB, an IscS/NifS homolog implications for its specificity toward selenocysteine"J. Biochem.. 131. 679-685 (2002)

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  • [Publications] K.Yoshimune, T.Yoshimura, T.Nakayama, T.Nishino, N.Esaki: "Hsc62, Hsc56, and GrpE, the third Hsp70 chaperone system of E. coli."Biochem. Biophys. Res. Commun.. 293. 1389-1395 (2002)

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  • [Publications] M.Kwak, H.Mihara, N.Esaki: "Protein interaction between selenocysteine lyase and MUP-I"Biomedical Research on Trace Elements. 13. 238-239 (2002)

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  • [Publications] E.Kuwana, S.Kurokawa, H.Mihara, T.Kurihara, T.Yoshimura, N.Esaki: "Effects of selenium deficiency on the expression of selenoprotein mRNAs in mouse brain"BiOMEDICAL RESEARCH ON TRACE ELEMENTS. 14. 293-296 (2003)

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  • [Publications] Y.Wei, T.Kurihara, T.Suzuki, N.Esaki: "A novel esterase from a psychrotrophic bacterium, Acinetobacter sp. strain no. 6, that belongs of the amidase signature family"J. Mol. Catal. B : Enzymatic. 23. 357-365 (2003)

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  • [Publications] T.Kurihara, T.Yamauchi, S.Ichiyama, H.Takahata, N.Esaki: "Purification, characterization, and gene cloning of a novel fluoroacetate dehalogenase from Burkholderia sp."FA1. J. Mol. Catal. B : Enzymatic. 23. 347-355 (2003)

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  • [Publications] Shibata, N. et al.: "Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase."J.Biol.Chem.. 278・25. 22717-22725 (2003)

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  • [Publications] Matsunami, H. et al.: "Chemical rescue of a site-specific mutant of bacterial copper amine oxidase for generation of the topa quinone cofactor."Biochemistry. 43・8. 2178-2187 (2004)

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  • [Publications] Ikushiro, H. et al.: "Reactions of serine palmitoyltransferase with serine and molecular mechanisms of the actions of serine derivatives as inhibitors."Biochemistry. 43・4. 1082-1092 (2004)

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  • [Publications] Omi, R.et al.: "Crystal structures of threonine synthase from Thermus thermophilus HB8:conformational change, substrate recognition, and mechanism."J.Biol.Chem.. 278・46. 46035-46045 (2003)

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  • [Publications] Nagano, S. et al.: "Infrared spectroscopic and mutational studies on putidaredoxin-induced conformational changes in ferrous CO-P450cam."Biochemistry. 42・49. 14507-14514 (2003)

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  • [Publications] Sato, H. et al.: "Hybridization of modified-heme reconstitution and distal histidine mutation to functionalize sperm whale myoglobin."J.Am.Chem.Soc.. 126・2. 436-437 (2004)

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  • [Publications] Goto, M.: "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action"J. Biol. Chem.. 277. 15890-15896 (2002)

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  • [Publications] Kim, M.: "X-ray Snapshots of Quinone Cofactor Biogenesis in Bacterial Copper Amine Oxidase"Nature Struct. Biol.. 9. 591-596 (2002)

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  • [Publications] Nakajima H: "Accurate measurement of near-micromolar oxygen concentrations in aqueous solutions based on enzymatic extradiol cleavage of 4-chlorocatechol : Applications to improved low-oxygen experimental systems and quantitative assessment of the back diffusion of oxygen from the atmosphere"J. Biochem.. 131. 523-531 (2002)

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  • [Publications] Hayashi, H.: "Conformational change in aspartate aminotransferase on substrate binding induces strain in the catlytic group and enhances catalysis"J. Biol. Chem.. 278. 9481-9488 (2003)

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  • [Publications] Shimokata, K.: "Asp51Asn mutation of bovine heart cytochrome c oxidase subunit I"Biochimi. Biophys. Acta. EBEC short report. 12. 105 (2002)

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  • [Publications] Kato.S.: "Cys328 of IscS and Cys63 of IscU are the site of disulfide bridge formation in a covalently-bound IscS/IscU complex : implication for the mechanism of iron-sulfur cluster assembly"Proc. Natl. Acad. Sci., USA. 99. 5948-5952 (2002)

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  • [Publications] Hirota, S.: "Spectroscopic obsevation of intermediates formed during the oxidative harf-reaction of copper/topa quinone-containing phenylethylamine oxidase"Biochemistry. 40・51. 15789-15796 (2001)

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  • [Publications] Datta, S.: "Structure of a quinohemoprotein amine dehydrogenase with an uncommon redox cofactor and highly unusual crosslinking"Proc. Natl. Acad. Sci. U.S.A.. 98・25. 14268-14273 (2001)

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  • [Publications] Mizuguchi, H: "Strain is More Important than Electrostatic Interaction in Controlling the pKa of the Catalytic Group in Aspartate Aminotransferase"Biochemistry. 40・2. 353-360 (2001)

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  • [Publications] Haruyama, K: "Structures of Escherichia coli Histidinol-Phosphate Aminotransferase and Its Complexes with Histidinol-Phosphate and N-(5'-Phosphopyridoxyl)-L-Glutamate : Double Substrate Recognition of the Enzyme"Biochemistry. 40・15. 4633-4644 (2001)

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  • [Publications] Okada, K: "Structures of Esherichia coli Branched-Chain Amigo Acid Aminotransferase and Its Complexes with 4-Methylvalerate and 2-Methylleucine : Induced Fit and Substrate Recognition of the Enzyme"Biochemistry. 40・25. 7453-7463 (2001)

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  • [Publications] Ikushiro, H: "A water-soluble homodimeric serine palmitoyltransferase from Sphingomonas paucimobilis EY2395T strain. purification, characterization, cloning, and overproduction"T. Biol. Chem.. 276・21. 18249-18256 (2001)

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Published: 2001-04-01   Modified: 2018-03-28  

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