| Project/Area Number |
13125203
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
ESAKI Nobuyoshi Kyoto Univ., Institute for Chemical Research, Professor, 化学研究所, 教授 (50135597)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATAKE Jun HIRATAKE,Jun, 化学研究所, 助教授 (80199075)
FUJII Ikuo Osaka Prefecture Univ., Research Institute for Advanced Science and Technology, Professor, 先端科学研究所, 教授 (70189984)
YOSHIMURA Tohru Nagoya, Univ., Graduate School of Bioagricultural sciences, 大学院・生命農学研究科, 教授 (70182821)
藤井 郁夫 生物分子工学研究所, 機能創製プロジェクト部門, 部長
畑 安雄 京都大学, 化学研究所, 助教授 (10127277)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥79,700,000 (Direct Cost: ¥79,700,000)
Fiscal Year 2003: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2002: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2001: ¥54,900,000 (Direct Cost: ¥54,900,000)
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| Keywords | composite biocatalyst / coenzyme / structure-activity relationship / pyridoxal phosphate / NADPH / iron-sulfur cluster / asymmetric synthesis / Suf / ジヒドロピリミジン / システインデスルフラーゼ / 鉄-硫黄クラスター / システインデルスフラーゼ / 抗体酵素 |
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| Research Abstract |
We analyzed the mechanism of biosynthesis of iron-sulfur clusters, which are useful as components of composite biocatalysts. We also developed novel processes for the production of chiral compounds by the use of novel composite biocatalysts. In addition, reaction mechanisms of several composite biocatalysts such as amino acid racemases which require pyridoxal phosphate as a coenzyme were clarified.
1. Sulfur atoms of iron-sulfur clusters are supplied from L-cysteine by the sulfur-elimination reaction catalyzed by cysteine desulfurases. We studied the detailed mechanisms of iron-sulfur cluster assembly involving the following cysteine desulfurases : IscS and SufS from Escherichia coli and SsCsd3 from Synechocystis sp. PCC6803. Interactions between these cysteine desulfurases and proteins functioning as scaffold proteins for the assembly of iron-sulfur clusters were analyzed.
2. We isolated and characterized a novel NADPH enzyme, N-methyl-L-amino acid dehydrogenase. The gene coding for this enzyme was cloned from Pseudomonas putida ATCC12633, and its overexpression system was constructed. Using this enzyme, we developed an enzymatic process in which chiral N-methyl-L-amino acids are produced from α-keto acids and methylamine.
3. We isolated and characterized a novel NADPH enzyme that ctalyzes the asymmetric reduction of a carbon-carbon double bond in halogenated unsaturated compounds. We developed an enzymatic process in which (S)-2-CPA, which is used as a building block for the synthesis of aryloxyphenoxypropionic acid herbicide, is produced by asymmetric reduction of a carbon-carbon double bond in 2-chloroacrylate.
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