New Chemical Approaches for Functional of DNA/RNA

• Project/Area Number: 13132101
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The University of Tokyo
• Principal Investigator: KOMIYAMA Makoto The University of Tokyo, Research Center for Advanced Science and Technology, Professor, 先端科学技術研究センター, 教授 (50133096)
• Co-Investigator(Kenkyū-buntansha): AIZAWA Masuo Tokyo Institute of Technology, Department of Bioscience and Biotechnology, Professor, 生命理工学部, 教授 (00016742) ; INOUE Shohei The University of Tokyo, Graduate School of Engineering, professor emeritus, 大学院工学系研究科, 名誉教授 (20010762) ; KUNITAKE Toyoki The University of Kitakyushu, Faculty of Environmental Engineering, Professor, 国際環境工学, 教授 (40037734)
• Project Period (FY): 2001 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥18,100,000 (Direct Cost: ¥18,100,000); Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2004: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 2003: ¥3,900,000 (Direct Cost: ¥3,900,000); Fiscal Year 2002: ¥4,300,000 (Direct Cost: ¥4,300,000); Fiscal Year 2001: ¥4,500,000 (Direct Cost: ¥4,500,000)
• Keywords: DNA / Artificial Restriction Enzyme / Functional Nucleic Acid / RNA / Peptide Nucleic Acid / Biopolvmer / Proteins / Ribozvme / 加水分解 / リボザイム / DNA分組織体 / DNA分子組織体 / 刺激応答性核酸 / バイオテクノロジー / 遺伝子操作 / アクリジン

Research Abstract

Prof. Aizawa, Prof. Inoue, and Prof. Kunitake estimated this research project, and gave some advices on the management as an assessment committee of this project. Prof. Komiyama endeavored to have the members of this project promote the exchanges of information with one another.
As an opportunity to be estimated by assessment committee and to discuss this project, the group meeting was held two times a year, and international meetings were also held at the time. Moreover, news letters and research results were disclosed on the web. Finally, the full report for this project was made and published.

Research Products

• [Publications] J.Sumaoka et al.: "Cooperation of the Ce(IV)/EDTA complex and oligoamine for prompt scission of DNA"Chem. Lett.. 20-21 (2001)
• [Publications] A.Kuzuya et al.: "New ribozyme-mimics employing Mg (II) ion as catalytic center"Chem. Lett.. 584-585 (2001)
• [Publications] K.Matsumura et al.: "Trinuclear Cu(II) complex for internucleoside linkage-specific cleavage of RNA"Nucleic Acids Res. Supple. 1. 85-86 (2001)
• [Publications] A.Kuzuya et al.: "Site-selective artificial ribonuclease using pinpoint RNA activation"Nucleic Acids Res. Supple. 1. 131-132 (2001)
• [Publications] M.Komiyama et al.: "Sequence-Selective Artificial Ribonucleases"Methods in Enzymology, Ribonucleases Part 1. 341. 455-468 (2001)
• [Publications] 小宮山 真: "化学が作る次世代のバイオテクノロジー"未来材料. 2. 10-11 (2002)
• [Publications] H.Asanuma et al.: "Photocontrol of DNA-duplex formation by using azobenzene-bearing oligonucleotides"Chem. Biochem.. 2. 39-44 (2001)
• [Publications] H.Asanuma et al.: "Spiropyran as a regulator of DNA hybridization with reversed switching mode to that of azobenzene"Chem. Lett.. 108-109 (2001)
• [Publications] T.Takarada et al.: "L-Threoninol as a chiral linker of azobenzene for the effective photo-regulation of DNA triplex formation"Chem. Lett.. 723-733 (2001)
• [Publications] H.Asanuma et al.: "Enantioselective incorporation of azobenzenes into oligodeoxyribonucleotide for the effective photo-regulation of duplex formation"Angew Chem. Int. Ed. Engl.. 40(14). 2671-2673 (2001)
• [Publications] X.Liang et al.: "Phenylazonaphthalene as a superb photo-regulator for DNA-triplex formation"Tetrahedron. Lett.. 42. 6723-6725 (2001)
• [Publications] X.Liang et al.: "Photo-regulation of DNA triplex formation by azobenzene"J. Am. Chem. Soc. 124. 1877-1883 (2001)
• [Publications] H.Asanuma et al.: "Photo-regulation of RNA polymerase reaction by use of modified DNA carrying an azobenzene"Nucleic Acids Res. Supple.. 1. 55-56 (2001)
• [Publications] H.Asanuma et al.: "Regulation of aggregated structure of dye molecules by DNA hybridization"Nucleic Acids Res. Supple.. 1. 193-194 (2001)
• [Publications] H.Asanuma et al.: "H-aggregation of Methyl Reds by the hybridization of DNA-dye conjugates"Chem. Lett.. 1. 85-86 (2001)
• [Publications] A.Kuzuya et al.: "Conjugation of various acridines to DNA for site-selective RNA scission by lanthanide ion"Bioconjugate Chem.. (印刷中).
• [Publications] M.Komiyama et al.: "Cutting DNA and RNA""Handbook on the Physics and Chemistry of Rare Earths". 35(印刷中).
• [Publications] 須磨岡 淳 他: "「化学フロンテイア:生命化学のニューセントラルドグマ」第4章 遺伝子切断"化学同人. 275 (2002)
• [Publications] 小宮山 真 他: "「科学便覧応用編」「バイオインスパイアードケミストリー」"丸善(印刷中).
• [Publications] M.Komiyama et al.: "Sequence-selective scission of DNA and RNA by lanthanide ions and their complexe in"Metal Ions in Biological Systems""Marcel Dekker(印刷中).