| Project/Area Number |
13307011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | RIKEN (2004)
Chiba University (2001-2003) |
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Principal Investigator |
TANIGUCHI Masaru RIKEN, Laboratory for Immune Regulation, Group Director, 免疫制御研究グループ, グループディレクター (80110310)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Michishige RIKEN, Laboratory for Immune Regulation, Scientist, 免疫制御研究グループ, 研究員 (20333487)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥55,250,000 (Direct Cost: ¥42,500,000、Indirect Cost: ¥12,750,000)
Fiscal Year 2004: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2003: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2002: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2001: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000)
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| Keywords | V α 14 NKT cells / IFN-γ / IL-4 / α-galactosylceramide / NKT細胞 / Vα14 / IL-10 / 制御性樹状細胞 / 抑制性NK受容体NKG2I / 新規NK受容体 / 自然免疫系 / 同種骨髄移植 / アポトーシス / 免疫制御 / Cタイプレクシン / DNAチップ / IgE抗原産生制御 / BCG / サブトラクション / 分化 / 自己免疫疾患 / 臓器移植生着 / α-ガラクトシルセラミド / NKT細胞特異的遺伝子 |
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| Research Abstract |
It is known that NKT cells are a critical and specific cells regulating the immune system which produce both IFN-γ and IL-4 by the injection of KRN7000 (α-Galactosylceramide). In this study, we have discovered that the antigen receptors on NKT cells rapidly down-regulate and NKT cells itself disappear in sight when KRN7000 bind to the receptors (Harada, et al.2004 Int Immunol). This result is significant in understanding the mechanism of NKT cells after the activation by KRN7000.
An important part was to identify genes responsible for NKT differentiation in a process known as microarray and subtraction. As a result, we have cloned a new receptor, KLRE-1,which is strongly expressed in NK and NKT cells rather than any other lymphocytes (Koike, et al.2004 JEM). By generating and analyzing KLRE-1 knockout mouse, we have found that this molecule is essential in rejection of allogenic cells (Shimizu, et al.2004 Blood).
It is also important to identify the role of NKT cells in human immunity. We have examined the relationship between other immuno-regulatory cells including dendritic cells and NKT cells. In this study, we found that a single stimulation of NKT cells give rise to the reaction of Th1-type response, while the multiple stimulation suppress the immunity. We have been trying to identifying the molecule which is responsible for this reaction.
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