Transcriptome Analysis of Atherosclerosis

• Project/Area Number: 13307023
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: KODAMA Tatsuhiko Research Center for Advanced Science and Technology, Professor, 先端科学技術研究センター, 科学技術振興特任教員(常勤形態) (90170266)
• Co-Investigator(Kenkyū-buntansha): WADA Yoichiro Komaba Open Laboratory, Research Assistant, 駒場オープンラボラトリー, 助手 (10322033) ; DOI Takefumi Osaka University, Graduate school of Pharmaceutical, Professor, 大学院・薬学研究科, 教授 (00211409)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥54,210,000 (Direct Cost: ¥41,700,000、Indirect Cost: ¥12,510,000); Fiscal Year 2002: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000); Fiscal Year 2001: ¥33,150,000 (Direct Cost: ¥25,500,000、Indirect Cost: ¥7,650,000)
• Keywords: DNA microarray / Atherosclerosis / transcription factor / GATA / WTAP / NFATc

Research Abstract

The changes in gene expression profiles during atherogenesis in vascular wall cells including vascular endothelial cell, vascular smooth muscle cell, monocyte and macrophage were studied using oligonucleotide DNA microarray. Following three targets were mainly analyzed comprehensively.
(1)Molecular mechanism of monocyte/endothelial cell interaction :
(2)Molecular mechanism of lipid accumulation in vascular smooth muscle cell :
(3)Transcriptome analysis of monocyte macrophage differentiation :

Research Products

• [Publications] Minami T, Abid MR, Zhang J, King G, Kodama T, Aird WC.: "Thrombin stimulation of VCAM-1 in endothelial cells is mediated by PKC-delta-NF-kappa B and PKC-zeta-GATA signaling pathways"J Biol Chem.. (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Santiago-Garcia J, Kodama T, Pitas RE.: "The class A scavenger receptor binds to proteoglycans and mediates adhesion of macrophages to the extracellular matrix"J Biol Chem.. (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takayanagi H, Kim S, Koga T.Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, Wagner EF, Mak TW, Kodama T, Taniguchi T.: "Induction and Activation of the Transcription Factor NFATc1 (NFAT2) Integrate RANKL Signaling in Terminal Differentiation of Osteoclasts"Dev Cell.. 3(6). 889-901 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Saiura A, Sugawara Y, Harihara Y, Sata M, Hamakubo T, Kodama T, Makuuchi M.: "Gene expression profile during acute rejection in rat-to-mouse concordant cardiac xenograft by means of DNA microarray"Transpl Int. 15. 535-540 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tanaka T, Takeno T, Watanabe Y, Uchiyama Y, Murakami T, Yamashita H, Suzuki A, Aoi R, Iwanari H, Jiang SY, Naito M, Tachibana K, Doi T, Shulman AI, Mangelsdorf DJ, Reiter R, Auwerx J, Hamakubo T, Kodama T.: "The Generation of Monoclonal Antibodies against Human Peroxisome Proliferator-activated Receptors (PPARs)"J Atheroscler Thromb.. 9(2). 233-242 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wada H, Hashimoto K, Wada Y, Kobayashi M, Izumi A, Sugiyama A, Kohro T, Hamakubo I, Kodama T: "Extensive oligonucleotide microarray transcriptome analysis of the rat cerebral artery and arachnoid tissue"J Atheroscler Thromb.. 9(2). 24-32 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Minami T, Abid MR, Zhang J, King G, Kodama T, Aird WC.: "Thrombin stimulation of VCAM-1 in endothelial cells is mediated by PKC-delta -NF-kappa B and PKC-zeta-GATA signaling pathways"J Biol Chem.. epub ahead of print. (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Santiago-Garcia J, Kodama T, Pitas RE.: "The class A scavenger receptor binds to proteoglycans and mediates adhesion of macrophages to the extracellular matrix"J Biol Chem.. epub ahead of print. (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, Wagner EF, Mak TW, Kodama T, Taniguchi T.: "Induction and Activation of the Transcription Factor NFATc1 (NFAT2) Integrate RANKL Signaling in Terminal Differentiation of Osteoclasts"Dev Cell.. 3(6). 889-901 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Saiura A, Sugawara Y, Harihara Y, Sata M, Hamakubo T, Kodama T, Makuuchi M.: "Gene expression profile during acute rejection in rat-to-mouse concordant cardiac xenograft by means of DNA microarray"Transpl Int.. 15. 535-540 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tanaka T, Takeno T, Watanabe Y, Uchiyama Y, Murakami T, Yamashita H, Suzuki A, Aoi R, Iwanari H, Jiang SY, Naito M, Tachibana K, Doi T, Shulman Al, Mangelsdorf DJ, Reiter R, Auwerx J, Hamakubo T,Kodama T.: "The Generation of Monoclonal Antibodies against Human Peroxisome Proliferator-activated Receptors(PPARs)"J Atheroscler Thromb.. 9. 233-242 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Wada H, Hashimoto K, Wada Y, Kobayashi M, Izumi A, Sugiyama A, Kohro T, Hamakubo T, Kodama T: "Extensive oligonucleotide microarray transcriptome analysis of the rat cerebral artery and arachnoid tissue"J Atheroscler Thromb.. 9. 224-232 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Minami T, Abid MR, Zhang J, King G, Kodama T, Aird WC.: "Thrombin stimulation of VCAM-1 in endothelial cells is mediated by PKC-delta-NF-kappa B and PKC-zeta-GATA signallng pathways"J Biol Chem.. (2002)
• [Publications] Santiago-Garcia J, Kodama T, Pitas RE.: "The class A scavenger receptor binds to proteoglycans and mediates adhesion of macrophages to the extracellular matrix"J Biol Chem.. (2002)
• [Publications] Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, Wagner EF, Mak TW, Kodama T, Taniguchi T.: "Induction and Activation of the Transcription Factor NFATc1(NFAT2)Integrate RANKL Signaling in Terminal Differenliation of Osteoclasts"Dev Cell.. 3(6). 889-901 (2002)
• [Publications] Saiura A, Sugawara Y, Harihara Y, Sata M, Hamakubo T, Kodama T, Makuuchi M.: "Gene expression profile during acute rejection in rat-to-mouse concordant cardiac xenograft by means of DNA microarray"Transpl lnt. 15. 535-540 (2002)
• [Publications] Tanaka T, Takeno T, Watanabe Y, Uchiyama Y, Murakami T, Yamashita H, Suzuki A, Aoi R, Iwanari H, Jiang SY, Naito M, Tachibana K, Doi T, Shulman Al, Mangelsdorf DJ, Reiter R, Auwerx J, Hamakubo T, Kodama T: "The Generation of Monoclonal Antibodies against Human Peroxisome Proliferator-acivated Receptors(PPARs)"J Atheroscler Thromb. 9(2). 233-242 (2002)
• [Publications] Wada H, Hashimoto K, Wada Y, Kobayashi M, Izumi A, Sugiyama A, Kohro T, Hamakubo T, Kodama T.: "Extensive oligonucleotide microarray transcriptome analysis of the rat cerebral artery and arachnoid tissue"J Atheroscler Thromb. 9(2). 24-32 (2002)
• [Publications] Nakamura T, Hinagata Ji J, Tanaka T, Imanishi T, Wada Y, Kodama T, Doi T: "HSP90, HSP70, and GAPDH Directly Interact with the Cytoplasmic Domain of Macrophage Scavenger Receptors"Biochem Biophys Res Commun. 290. 858-864 (2002)
• [Publications] Hippo Y, Taniguchi H, Tsutsumi S, Machida N, Chong JM, Fukayama M, Kodama T, Aburatani H: "Global Gene Expression Analysis of Gastric Cancer by Oligonucleotide Microarrays"Cancer Res. 62. 233-240 (2002)
• [Publications] Mashiba S, Wada Y, Takeya M, Sugiyama A, Hamakubo T, Nakamura A, Noguchi N, Niki E, Izumi A, Kobayashi M, Uchida K, Kodama T: "In Vivo Complex Formation of Oxidized alpha(1)-Antitrypsin and LDL"Arterioscler Thromb Vasc Biol. 21. 1801-1808 (2001)
• [Publications] Takabe W, kodama T, Hamakubo T, Tanaka K, Suzuki T, Aburatani H, Matsukawa N, Noguchi N: "Anti-atherogenic antioxidants regulate the expression and function of proteasome {alpha}-type subunits in human endothelial cells"J Biol Chem. 276. 40497-40501 (2001)
• [Publications] Husemann J, Obstfeld A, Febbraio M, Kodama T, Silverstein SC.: "CD11b/CD18 mediates production of reactive oxygen species by mouse and human macrophages adherent to matrixes containing oxidized LDL"Arterioscler Thromb Vasc Biol. 21. 1301-1305 (2001)
• [Publications] Umetani M, Mataki C, Minegishi N, Yamamoto M, Hamakubo T, Kodama T.: "Function of GATA transcription factors in induction of endothelial vascular cell adhesion molecule-1 by tumor necrosis factor-alpha"Arterioscler Thromb Vasc Biol. 21. 917-922 (2001)