| Project/Area Number |
13357015
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ETO Kazuhiro Tokyo Medical and Dental University, Section of Molecular Craniofacial Embryology, Professor, 大学院・医歯学総合研究科, 教授 (30014161)
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| Co-Investigator(Kenkyū-buntansha) |
OTA Masato Tokyo Medical and Dental University, Section of Molecular Craniofacial Embryology, Assistant Professor, 大学院・医歯学総合研究科, 助手 (70313228)
ISEKI Sachiko Tokyo Medical and Dental University, Section of Molecular Craniofacial Embryology, Assistant Professor, 大学院・医歯学総合研究科, 助手 (80251544)
IIMURA Tadahiro Tokyo Medical and Dental University, Section of Molecular Craniofacial Embryology, Assistant Professor, 大学院・医歯学総合研究科, 助手 (20282775)
IKEDA Masa-aki Tokyo Medical and Dental University, Section of Molecular Craniofacial Embryology, Associate Professor, 大学院・医歯学総合研究科, 助教授 (20193211)
NAKAHARA Taka Tokyo Medical and Dental University, Section of Molecular Craniofacial Embryology, Research Associate, 歯学部, 非常勤講師 (10366768)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2004: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2003: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2002: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2001: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Keywords | Cranial Neural Crest Cells / Cranial Neural Crest Stem Cells / in situ Tissue Engineering / Cell-based therapy / Cell proliferation / Cell differentiation / Twist / Tissue regeneration / 再生 / 幹細胞 / 細胞分離 / 細胞培養 / 哺乳類頭部神経堤幹細胞 / 頭蓋顔面部の組織再生 / LacZ遺伝子導入マウス / 凍結保存 / DNazyme / 哺乳類頭部神経堤細胞 / 頭部間葉 / 全胚培養 / GFP遺伝子導入マウス / 全胚培養と上顎器官培養 |
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| Research Abstract |
We tried isolation and isolation of Cranial Neural Crest Stem Cells for the cell-based therapy in the future indicated as below.
1)Identification of the stem cell niche of Neural Crest Cells
a)Distribution pattern of Cranial Neural Crest Cells during Craniofacial morphogenesis in Wnt-1/Cre mouse.
The distribution pattern of Cranial Neural Crest Cells (CNCCs) was examined in Wnt-1/Cre mouse, which indicate b-galactosidase activity in Neural Crest Cells, during craniofacial morphogenesis. B-galactosidase positive CNCCs were identified in the frontal and facial bone, and these pattern were constructed until E9.5.
b)Method for identification of Mesenchymal Stem cells in Craniofacial region by chasing the Label-Retaining Cells (LRCs).
2)Isolation of Cranial Neural Crest Stem Cells (CNCSCs).
a)Modification the isolation methods of CNCSCs
3)Molecular understanding of cell proliferation and apoptosis in cranial neural crest cells
a)Expression pattern of cell-cycle regulators in developing cranial sutu
… More
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b)Cell cycle regulation in terminal-differentiated cells in
c)Twist-dependent regulation of cell proliferation and apoptosis
We examined Twist function in CNCCs in Twist null mice and found that Twist is essential for the Cranial nerve patterning, cell growth, and anti-apoptotic activity in CNCCs. We also developed the knockdown methods of Twist activity in vitro by DNazyme and, antisense morpholino nucleotide methods.
d)The transcriptional regulation of cell-cycle regulator E2F1.
e)Cell-cycle related acetylation of the p220/NPAT by CBP histone acetyltransferase
4)Clinical potentialities of Cranial Neural Crest Cells
a)Periodontal tissue regeneration by in situ tissue engineering by the combination of growth factors and scaffolds
b)Periodontal tissue regeneration by in situ tissue engineering by the combination of cell and scaffolds
We examined the clinical potentialities of cranial neural crest cells by tissue engineering methods, and data suggested that they will be a powerful tool in cell-based therapy in the future.
5)Gene expression profiling of the Cranial Neural Crest Stem Cells Less
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