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Analysis of temperature-sensitive oscillatory system in Drosophila circadian clock mechanism

Research Project

Project/Area Number 13440250
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionOKAYAMA UNIVERSITY (2003)
Yamaguchi University (2001-2002)

Principal Investigator

TOMIOKA Kenji  Okayama University, Faculty of Science, Professor, 理学部, 教授 (30136163)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥6,500,000 (Direct Cost: ¥6,500,000)
KeywordsDrosophila / circadian clock / temperature sensitive oscillator / multi-oscillator system / circadian rhythm / clock neurons / cryptochrome / 温度感受性振動機構 / 複数振動体系 / 温度周期 / 時計分子 / 時計タンパク / PERIOD / 時計タンパク質 / 光周期
Research Abstract

The purpose of this study was to clarify the oscillatory mechanism of Drosophila temperature sensitive oscillator and how this oscillator cooperates with a light-entrainable oscillator to produce a stable locomotor rhythm. When transferred from temperature cycle (TC) under constant light (LL) to constant temperature, wild type files maintained locomotor rhythms for a few cycles. The rhythm was induced by temperature step-down from 25℃ or 30℃ to 20℃. The rhythm was associated with rhythmic expression of PER and TIM, suggesting that temperature step-changes induce oscillation of circadian clock through quantitative change in clock molecules. By a series of experiments using arrhythmic clock mutant flies, it was also suggested that the temperature-induced oscillation requires CLK and CYC. Immunohistochemistry with anti-PER antibody revealed that neurons located in the lateral protocerebrum (LNs) showed PER during the cryophase and that nuclear transport of PER occurred during the late cryophase. disco mutant flies lacking LNs did not show endogenous rhythms under TC. These data suggest that LNs play major roles in generating the circadian locomotor rhythm under temperature cycles. cry^b mutant flies that lack functional CRY exhibited internal splitting of locomotor rhythms into a shorter period component (SPC) with a free-running period (τ) of about 22.5 hr and a longer period component (LPC) with τ of about 25 hr. The two oscillations were coupled in LD and DD conditions and SPC seemed predominant in coupled condition. Immunohistochemistry revealed that SPC was driven by LNs and some fraction of so called dorsal neurons (DNs) and LPC by the rest of DNs. Simultaneous entrainment by temperature cycles and light cycles showed that LPC was light entrainable and SPC more temperature entrainable. These results suggest that the temperature sensitive oscillator located in LNs and some DNs that synchronizes the light sensitive oscillator to its own phase.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Tomioka, Kenji: "Circadian organization in multioscillator systems in insects"Comparative Biochemistry and Physiology A. 130. 871-871 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshii, Taishi: "A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster."Zoological Science. 19. 841-850 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "Photoperiodic modulation of insect circadian rhythms"Journal of Photoscience. 9. 9-12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshii, Taishi: "Possible involvement of temperature-entrainable timing system in arrhythmic mutant flies in Drosophila melanogaster"Journal of Photoscience. 9. 240-242 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富岡 憲治: "複数振動体系による昆虫概日リズムの構築"比較生理生化学. 20. 140-146 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshii, Taishi: "Drosophila cry^b mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light"Journal of Insect Physiology. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富岡 憲治: "生物時計--環境への時間的調和の手段,井上慎一他編「時間と時」"学会出版センター. 159-172 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "Circadian organization in multioscillator systems in insects"Comparative Biochemistry and Physiology A. 130. 871 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshii, Taishi: "A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster"Zoological Science. 19. 841-850 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "Photoperiodic modulation of insect circadian rhythms"Journal of Photoscience. 9. 9-12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshii, Taishi: "Possible involvement of temperature-entrainable timing system in arrhythmic mutant flies in Drosophila melanogaster"Journal of Photoscience. 9. 240-242 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "Biological clock-A way of temporal harmonization to environment (in Japanese)"Jikan to Toki (ed, by H.Hironaka, S.I.Inouye and T.Kaneko) (Gakkai Shuppan Center). 159-172 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "Multi-oscillator system controlling locomotor rhythm in insects."Circadian Clock as Multi-Oscillator System (ed. By K.Honma and S.Honma) (Hokkaido University Press). 61-74 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "Regulation of insect circadian rhythms by multi-oscillator systems (in Japanese)"Comparative Physiology and Biochemistry. 223 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshii, Taishi: "Drosophila cry^b mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light"Journal of Insect Physiology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomioka, Kenji: "An Introduction to Chronobiology"Shokabo. 223 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富岡 憲治: "複数振動体系における昆虫概日リズムの構築"比較生理生化学. 20. 140-146 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshii, Taishi: "Drosophila cry^b mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light"Journal of Insect Physiology. 50(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomioka, Kenji: "Multioscillator systems controlling the circadian locomotor rhythm in insects. In K.Honma and S.Honma(eds)Circadian Clock as Multi-oscillation system"Hokkaido University Press. 61-74 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 富岡 憲治: "時間生物学の基礎"裳華房. 223 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshii T, Sakamoto M, Tomioka K: "A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster"Zoological Science. 19. 841-850 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomioka K, Uwozumi K, Koga M: "Photoperiodic modulation of insect circadian rhythms"Journal of Photoscience. 9. 9-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshii T, Tomioka K: "Possible involvement of temperature-entrainable timing system in arrhythmic mutant flies in Drosophila melanogaster"Journal of Photoscience. 9. 240-242 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomioka K, Yoshii T, Saifullah ASM: "Multioscillator systems controlling the circadian locomotor rhythm in insects. Proceedings of Sapporo Symposium on Biological Rhythm (ed by K.Honma and S.Honma)"Hokkaido University Press(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamoto A, Mori H, Tomioka K: "The role of optic lobe in circadian locomotor rhythm generation in the cricket Gryllus bimaculatus, with special reference to PDH-immunoreactive neurons"Journal of Insect Physiology. 47. 889-895 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsushima A, Chuman Y, Onoue H, Ito Y, Tomioka K: "Gene expression of a circadian pacemaker hormone peptide PDF in cricket Gryllus bimaculatus"Peptide Seience. 2000. 63-66 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saifullah ASM, Tomioka K: "Serotonin sets the day state in the neurons that control coupling between the optic lobe circadian pacemakers in the cricket, Gryllus bimaculatus"Journal of Experimental Biology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomioka K, Saifullah ASM, Koga M: "The circadian clock system of hemimetabolous insects. In : Insect Timing : Circadian Rhythmicity to Seasonality. (Denlinger DL, Giebultowicz JM, Saunders DS eds)"Elsevier Science BV. 43-54 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 富岡憲治: "生物時計-環境への時間的調和の手段、井上慎一他編「時間と時」"学会出版センター. 159-172 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomioka K, Yoshii T, Saifullah ASM: "Multioscillator systems controlling the circadian locomotor rhythm in insects. Proceedings of Sapporo Symposium on Biological Rhythm (ed by K.Honma and S.Honma)"Hokkaido University Press(印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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