Study on scaling theory of on-chip electrophoretic micro-capillary and its miniaturization and optimization
| Project/Area Number |
13450146
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
電子デバイス・機器工学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KUROKI Yukinori Department of Electronics, Department of Electronics, Graduate School of Information Science and Electrical Engineering, Professor, 大学院・システム情報科学研究院, 教授 (40234596)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Reiji KYUSHU UNIVERSITY, Department of Electronics, Graduate School of Information Science and Electrical Engineering, Associate Professor, 大学院・システム情報科学研究院, 助教授 (60221503)
IKEDA Akihiro KYUSHU UNIVERSITY, Department of Electronics, Graduate School of Information Science and Electrical Engineering, Research Associate, 大学院・システム情報科学研究院, 助手 (60315124)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2001: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | Microfabrication / Photosensitive Glass / Confocal Laser Scanning Microscope / Electrophoresis / DNA Analvsis / Polysilazane / μTAS / MEMS / マイクロキャピラリーチップ / アクリルアミドコーティング / 電気浸透流 / アミノ酸 / DNAラダー |
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| Research Abstract |
In this research, an on-chip microcapillary was formed on a new substrate material. Fabrication involved the use of a confocal laser scanning microscope, microfabrication processes and utilized the action of analyte in the micro fluidic device. The ability of the on-chip microcapillary to perform electrophoresis analysis on biological substances including DNA was investigated in detail.
The processing characteristics of photosensitive glass, as a substrate material, were studied in detail. Tests were conducted by applying this substrate as an actual device to determine the feasibility of applying this substrate to a microdevice. Polysilazane solution was used to smooth the surface of the fluidic channel and to achieve adhesion between the substrate and upper cover glass. This demonstrates that it is a promising material for application to future fluidic devices.
Using such new materials, on-chip electrophoresis capillary was fabricated. This capillary can carry out separate analysis of biological substances. Electrophoresis separation was carried out and analyzed for a fluorescent dye substance, amino acids, and DNA. In each analysis, the result of multiple conventional electrophoresis analysis could be carried out simultaneously thus significantly shortening the time required. In addition, since analysis can be done simultaneously, a reduction in the amount of test material required is achieved. Using a conventional optical microscope, the situation of the electrophoresis phenomenon inside the capillary was observed in detail using the confocal laser scanning microscope. In addition to this, the electric field distribution in the capillary at the time of electrophoresis was simulated using the semiconductor device simulator MEDICI. The simulation was successful in observing the difference in the internal electric field distribution by comparing with observed results.
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Report
(5 results)
Research Products
(11 results)
