• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Microbial Community Analyses of Municipal Solids Waste Landfill Site Soils by 16S rRNA molecular approach

Research Project

Project/Area Number 13450214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionNagaoka University of Technology

Principal Investigator

HARADA Hideki  Nagaoka University of Technology, Faculty of Engineering, Professor, 工学部, 教授 (70134971)

Co-Investigator(Kenkyū-buntansha) ARAKI Nobuo  Nagaoka University of Technology, Faculty of Engineering, Assoc. Professor, 環境都市工学科, 助教授 (30193072)
OHASHI Akiyoshi  Nagaoka University of Technology, Faculty of Engineering, Assoc. Professor, 工学部, 助教授 (70169035)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥9,100,000 (Direct Cost: ¥9,100,000)
Keywords16S rDNA / Archaea / diversity / landfill / Homoacetogenesis / * / Global warming / fluorescence in-situ hybridization / 温室化効果ガス
Research Abstract

Archaeal 16S rDNA clones libraries were constructed for samples taken at 10, 20 and 30m depth in a municipal solid waste landfill, which corresponded approximately 3, 6 and 9 years operation, respectively. Sequencing and phylogenic analyses of representative clones showed all of the rDNAs were closely related to typical methanogens. The distributions of phylotypes in clones libraries were similar to each other. Dominant clones in all the clone libraries were closely related to thermophilic species, such as Methanothermobacter thermoautotrophicus, suggesting that the temperature at these sites were high. This was supported by the results of H2-dependent methanogenic activity tests, showing that the activities of all samples at 55℃ were much higher than those at 25℃.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Chen, A.-C., Imachi, H., Sekiguchi, Y, Ohashi, A., Harada, H.: "Archaeal community composition at different depth (up to 30m) of a municipal solid waste landfill in Taiwan as revealed by 16SrDNA cloning analyses"Biotechnology Letters. 25. 719-724 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chen, A.-C., Ohashi, A, Harada.H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. 24. 435-444 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chen, A.-C., Imachi, H., Sekiguchi, Y., Ohashi, A., Harada, H.: "Archaeal community composition at different depth (up to 30 m) of a municipal solid waste landfill in Taiwan as revealed by 16S rDNA cloning analyses"Biotechnology Letters. 25. 719-724 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chen, A.-C., Ohashi, A., Harada, H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. 24. 435-444 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chen, A.-C., Imachi, H., Sekiguchi, Y., Ohashi, A., Harada, H.: "Archaeal community composition at different depth(up to 30m)of a municipal solid waste landfill in Taiwan as revealed by 16S rDNA cloning analyses"Biotechnology Letters. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chen, A.-C., Ohashi, A., Harada, H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 澤田浩介, 原田秀樹, 大橋晶良: "異なる酸化還元条件下の埋立地模擬カラムを用いた有機性廃棄物の初期分解過程の挙動"平成14年度土木学会年次学術講演会. Vol.7. VII-130 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Imachi, H., Sekiguchi, Y., Kamagata, Y., Hanada, S., Ohashi, A., Harada H.: "Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium"Int. J. Syst. Evol. Microbiol.. Vol.52. 1729-1735 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 澤田浩介, 原田秀樹, 大橋晶良: "廃棄物埋立地模擬カラムによる有機物分解と温室効果ガス放出の挙動"第37回日本水環境学会年会講演集. Vol.37. 524 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 多川正, 関口勇地, 荒木信夫, 大橋晶良, 原田秀樹: "UASB廃水処理プロセスでの嫌気性微生物群集の分子生物学的手法による動態解析"環境工学研究論文集. 38. 151-162 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Sekiguchi, H.Takahashi, Y.Kamagata, A.Ohashi, H.Harada: "In situ Detection, IsoIation and Physiological Properties of a Thin Filamentous Microoganism Abundant in Methanogenic Granular Sludges : a Novel Isolate affiliated with a Clone Cluster, the Green Non Sulfur Bacteria Subdivision 1"Applied and Environmental Microbiology. 67. 5740-5749 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Sekiguchi, Y.Kamagata, H.Harada: "Recent Advances in Methane Fermentation Technology"Current Opinion in Biotechnology. Vol.12 No.3. 277-282 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi T., Uemura S., I-Cheng Tseng, Ohashi A., Harada H.: "Microbial-Ecological Significance of Sulfide Precipitation within Anaerobic Granular Sludge Reveled by Micro-electrodes Study"Water Research. Vol.35 No.14. 3411-3417 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shutsubo K., Sinthurat N., Ohashi A., Harada H.: "Population Dynamics of Anaerobic Microbial Consortia in Thermophilic Granular Sludge in Response to Feed Composition Change"Water Science and Technology. Vol.43 No.1. 59-66 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi