SHIRATAKE Katsuhiro NAGOYA UNIVERSITY, GRADUATE SCHOOL OF BIOAGRICULTURAL SCIENCE, ASSOCIATE PROFESSOR, 大学院・生命農学研究科, 助教授 (90303586)
MORI Hitoshi NAGOYA UNIVERSITY, GRADUATE SCHOOL OF BIOAGRICULTURAL SCIENCE, PROFESSOR, 大学院・生命農学研究科, 教授 (20220014)
|Budget Amount *help
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2002: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2001: ¥9,600,000 (Direct Cost: ¥9,600,000)
The purpose of this project is to make clear the genes preferentially relating to parthenocarpy and fruit setting for its stabilization.
It was obserbed using fluorescence microscopy that pollen tube elongated after pollination of eggplant flower and the tip reached the ovules in ovary within 1.5 to 2 days after pollination. Therefore, three experimental conditions of pollination, unpollination, and unpollination + auxin treatments were set after emasculation on the flower opening. And RNAs from each sample were extracted after 2 or 3 days growth. Regarding the expression on 2 days after pollination, subtracted cDNA library was prepared by the subtraction between pollinated and unpollinated fruit. We picked up randumly 384 cDNA clones and spotted them on the membrane for macroarray assay. Thirty two candidates of cDNA clones for genes expressed higher in pollinated fruit than unpollinated one were selected and used as probes for Nothern blot analysis. Eleven of these 32 cDNA clones show
ed that hybridization signals with mRNA from pollinated fruit were stronger ttian that from unpollinated fruit. mRKAs of three genes having similarity to P450, Unknown protein, and Hypothetical protein were specifically expressed in both pollinated and auxin treated fruit from 2 to 4 days after anthesis and then reduced rapidly. On the other hand, regarding the expression on 3 days after pollination, there were 10 cDNA clones expressed preferentially, in which histone, carleticulin, polyphenol oxidase, cell wall structural protein, cell division relating protein, molecular chaperon were contained. However, almost all mRNAs continued to be expressed clearly ever after 3 days. Thus, it was suggested that the 3 genes expressed on 2 days related to the initiation of fruit set.
In sweet pepper fruit, also, 7 EST genes preferentially expressed in pollinated fruit on 2 days after pollination were isolated using the same way to eggplant fruit. Embryomic flower 2, which functions the regulation of flowering, was prominently expressed by pollination. Less