| Project/Area Number |
13460119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
KAWAMURA Osamu UNIVERSITY OF MIYAZAKI, FACULTY OF AGRICULTURE, PROFESSOR, 農学部, 教授 (00041062)
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Mitsuhiro UNIVERSITY OF MIYAZAKI, FACULTY OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助手 (20305064)
AKASHI Ryo UNIVERSITY OF MIYAZAKI, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (20253809)
FUKUYAMA Kiichi UNIVERSITY OF MIYAZAKI, FACULTY OF AGRICULTURE, PROFESSOR, 農学部, 教授 (50238515)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2001: ¥10,300,000 (Direct Cost: ¥10,300,000)
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| Keywords | BREEDING / CULTIVATION OF CALLI / SUSPENSION CULTURE / HIGH DIGESTIBILITY / SOMACLONAL VARIATION / RUMINANT FEED / RICE PLANT |
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| Research Abstract |
In order to produce the high quality rice plant for ruminant feed by the tissue culture technique, we carried out the experiments as follows ;
The calli were induced from the scutellum of matured rice seed (cv. Nihonbare). At 28 days after initial cultivation, the embryogenic structures covered with the compact callus was formed. The successive cultivation regenerated the 89 plants from the re-differentiation medium through the formation of somatic embryogenesis.
Fifty-three regenerated plans were randomly selected, transplanted into pots (1/5000a) and transferred to the outdoors. Thirty-seven control plants, which were obtained from the individual seed of Nihonbare, were grown under the same conditions. The regenerated plants have a tendency to be late for the ear emergence. Additionally, they have more number of ear, less seeds weight and lignin content and higher leaf/stem ratio, compared with the control. It was not observed the significant differences in the plant length, the dry ma
… More
tter yield, the dry matter digestibility and the digestible dry matter yield between the regenerants and the control. In the most of measurements, the range of values is wider in the regenerant than in the control and the distribution pattern is different between two groups.
Successively, the promising 3 lines were selected from the regenerated plants and were grown under the same condition above. The term of ear emergence of in the regenerant group is longer than the control groups. One of 3 lines produced more the digestible dry matter than the control groups. The digestible dry matter yields were more variable in the regenerant group. The weights of seed in the regenerants were equivalent or less compared with control. In the most of measurements, the range of values is wider in the regenerant than in the control and the distribution pattern is different between two groups. It was recognized that some plants in the regenerant group have the much seed and digestible dry matter in leaf and stem, and have the remarkable digestible dry matter though having less seed. We obtained the higher digestible rice plants in the regenerant plants than in the control "Nihonbare", but are required the successive experiment because the trait is not fixed yet. Less
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