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Restoration of various species of Galliformes using chickens

Research Project

Project/Area Number 13460132
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionShinshu University

Principal Investigator

ONO Tamao  Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (10177264)

Co-Investigator(Kenkyū-buntansha) KAGAMI Hiroshi  Shinshu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80308303)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥16,900,000)
Fiscal Year 2003: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordschicken / quail / Blue-breasted quail / embryogenesis / primordial germ cells / chimera / PCR / in situ hybridization
Research Abstract

In order to rescue the endangered species, and production and preservation of variable breeds in bird, interspecific transfers of primoridal germ cells (PGCs) have been performed. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. We have worked toward practical use of PCR and in situ hybridization to identify the species specific cell (genome) type among chickens, quail and blue-breasted quail. PGCs of chick embryos were transferred into quail embryos, and the chicken-genome-specific PCR product was observed in the semen of chick-PGC-transferred chimeric quail. We made a progeny test. Females of the putative chimeras were artificially inseminated with chicken semen. We obtained hatchlings but PCR analysis showed that they were hybrid between chicken sperm and donor derived quail ovum. Silky PGCs were introduced to White Leghorn chickens and the prpgeny test is underway.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Li, H.C., Matsui, K., Ono, T.: "Population of circulating primordial germ cells in early Japanese quail embryos."Journal of Poultry Science. 38(2). 175-180 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Obata, K, Koide, M., Nagata, K., Iio, A., Yazawa, S., Ono, T., Yamada, Y., Tuan, R.S., Yokota, M.: "Role of FK506-binding protein 12 in development of the chick embryonic heart."Biochemical and Biophysical Research Communications. 283(3). 621-626 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, H.C., Kagami, H., Matsui, K., Ono, T.: "Restriction of proliferation of primordial germ cells by the irradiation of Japanese quail embryos with soft X-rays."Comparative Biochemistry and Physiology Part A. 130(1). 133-140 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono, T.: "Ex ovo culture of quail embryos and its application for embryo manipulation."Animal Science Journal. 72(5). 361-371 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, H.C., Zhao, L., Kagami, H., Matsui, K., Ono, T.: "Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products."Journal of Poultry Science. 38(4). 308-316 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Harumi, T., Maruyama, K., Kagami, H., Sano, A., Matsubara, Y., Tagami, T., Naito, M.: "Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR."Animal Genetics. 32(6). 386-389 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H., Iwata, J., Yasuda, J., Ono, T.: "Strain preference in donor and recipient for production of W-bearing sperm in mixed-sex germline chimeric chickens."Comparative Biochemistry and Physiology Part A. 131(2). 287-292 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H.: "Developmental genetic analysis of the avian primordial germ cells and the applications for production of chimeric chickens."Journal of Poultry Science. 39(3). 131-139 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, Z.D., Liu, C.H., Huang, J.S., Sha, J., Wei, H., Sun, M.J., Zhao, C., Jiang, S.H., Kagami, H.: "Preparation of interspecific chicken (Gallus Domesticus)/duck (Anas Domesticus) chimeras."Acta Zoologica Sinica. 48(4). 543-548 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Obata, K., Koide, M., Nagata, K., Iio, A., Yazawa, S., Ono, T., Sasaki, S., Yamada, Y., Tuan, R.S., Yokota, M.: "Effects of FK506 and rapamycin on formation of the neural tube in chick embryos."Animal Science Journal. 73(3). 229-234 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H., Yasuda, J., Tagami, T., Naito, M., Matsubara, Y., Harumi, T., Noguchi, T., Yamamoto, Y., Takahashi, T., Matsuyama, J., Komatsu, H., Ono, T.: "Effect of the strain combination in donor and recipient for production efficiency of the W-bearing sperm in mixed-sex germline chimeric chickens."Animal Science Journal. 73(6). 543-456 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., Kagami, H., Ono, T.: "Heart-selective expression of the chicken FK506-binding protein (FKBP) 12.6 gene during embryonic development."Developmental Dynamics. 226(1). 33-41 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., Kagami, H., Ono, T.: "Gene expression of FK506-binding proteins 12.6 and 12 during chicken development."Comparative Biochemistry and Physiology, Part A. 136(2). 391-399 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hrabia, A., Takagi, S., Ono, T., Shimada, K.: "Fertilization and development of quail oocytes after intracytoplasmic sperm injection."Biology of Reproduction. 69(5). 1651-1657 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H.: "Sex reversal in chicken."World's Poultry Science Journal. 59(1). 15-18 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 鏡味裕: "生殖前駆細胞の鳥類生命工学への応用"北信越畜産学会報. 86. 1-7 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, J.N., Kim, M.A., Park, T.S., Kim, D.K., Ono, T., Lim, J.M., Han, J.Y.: "Enriched gonadal migration of donor-derived primordial germ cells by immunomagnetic cell sorting in birds."Molecular Reproduction and Develolment. 68(1). 81-87 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, M.A., Park, T.S., Kim, J.N., Kim, Park, H.J., Lee, Y.M., Ono, T., Lim, J.M., Han, J.Y.: "Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos"Theriogenology. 62(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, H.C., Matsui, K., Ono, T.: "Population of circulating primordial germ cells in early Japanese quail embryos."Journal of Poultry Science. 38(2). 175-180 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Obata, K, Koide, M., Nagata, K., Ito, A., Yazawa, S., Ono, T., Yamada, Y., Tuan, R.S., Yokota, M.: "Role of FK506-binding protein 12 in development of the chick embryonic heart."Biochemical and Biophysical Research Communications. 283(3). 621-626 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, H.C., Kagami, H., Matsui, K., Ono, T.: "Restriction of proliferation of primordial germ cells by the irradiation of Japanese quail embryos with soft X-rays."Comparative Biochemistry and Physiology Part A. 130(1). 133-140 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono, T.: "Ex Ovo Culture of Quail Embryos and its Application for Embryo Manipulation."Animal Science Journal. 72(5). 361-371 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, H.C., Zhao, L., Kagami, H., Matsui, K., Ono, T.: "Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products."Journal of Poultry Science. 38(4). 308-316 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Harumi, T., Maruyama, K., Kagami, H., Sano, A., matsubara, Y., Tagami, T., Naito, M.: "Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products."Animal Genetics. 32(6). 386-389 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H., Iwata, J., Yasuda, J., Ono, T.: "Strain preference in donor and recipient for production of W-bearing sperm in mixed-sex germiline chimeric chickens."Comparative Biochemistry and Physiology Part A. 131(2). 287-292 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H.: "Developmental genetic analysis of the avian primordial germ cells and the applications for production of chimeric chickens."Journal of Poultry Science. 39(3). 131-139 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, Z.D., Liu, C.H., Huang, J.S., Sha, J., Wei, H., Sun, M.J., Zhao, C., Jiang, S.H., Kagami, H.: "Preparation of interspecific chicken (Gallus Domesticus)/duck (Anas Domesticus) chimeras."Acta Zoologica Sinica. 48(4). 543-548 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Obata, K., Koide, M., Nagata, K., Iio, A., Yazawa, S., Ono, T., Sasaki, S., Yamada, Y., Tuan, R.S., Yokota, M.: "Effects of FK506 and rapamycin on formation of the neural tube in chick embryos."Animal Science Journal. 73(3). 229-234 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H., Yasuda, J., Tagami, T., Naito, M., Matsubara, Y., Harumi, T., Noguchi, T., Yamamoto, Y., Takahashi, T., Matsuyama, J., Komatsu, H., Ono, T.: "Effect of the strain combination in donor and recipient for production efficiency of the W-bearing sperm in mixed-sex germline chimeric chickens."Animal Science Journal. 73(6). 453-456 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., Kagami, H., Ono, T.: "Heart-selective expression of the chicken FK506-binding protein (FKBP) 12.6 gene during embryonic development."Developmental Dynamics. 226(1). 33-41 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., kagami, H., Ono, T.: "Gene expression of FK506-binding proteins 12.6 and 12 during chicken development."Comparative Biochemistry and Physiology, Part A.. 136(2). 391-399 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hrabia, A., Takagi, S., Ono, T., Shimada, K.: "Fertilization and development of quail oocytes after intracytoplasmic sperm injenction."Biology of Reproduction.. 69(5). 1651-1657 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, J.N., Kim, M.A., Park, T.S., Kim, D.K., Ono, T., Lim, J.M., Han, J.Y.: "Enriched gonadal migration of donor-derived primordial germ cells by immunomagnetic cell sorting in birds."Molecular Reproduction and Develolment.. 68(1). 81-87 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagami, H.: "Sex reversal in chicken."World's Poultry Science Journal. 59(1). 15-18 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 鏡味裕: "生殖前駆細胞の鳥類生命工学への応用"北信越畜産学会報. 86. 1-7 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, M.A., Park, T.S., Kim, J.N., Kim, Park, H.J., Lee, Y.M., Ono, T., Lim, J.M., Han, J.Y.: "Production of quail (Conturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos."Theriogenology.. 62(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yazawa, S.(筆頭著者): "Gene expression of FK506-binding proteins 12.6 and 12 during Chicken development"Comparative Biochemistry and Physiology, Part A.. 136(2). 391-399 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hrabia, A.(筆頭著者): "Fertilization and development of quail oocytes after intracytoplasmic sperm injection"Biology of Reproduction. 69(5). 1651-1657 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kim, J.(筆頭著者): "Enriched gonadal migration of donor-derived primordial germ cells by immunomagnetic cell sorting in birds"Molecular Reproduction and Develolment. 68(1). 81-87 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kim, M.A., Park, T.S., Kim, J.N., Kim, Park, H.J., Lee, Y.M., Ono, T., Lim, J.M., Han, J.Y.: "Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos"Theriogenology. 62(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kagami, H.: "Strain preference in donor and recipient for production of W-bearing sperm in mixed-sex germline chimeric chickens"Comparative Biochemistry and Physiology Part A. 131(2). 287-292 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Obata, K.: "Effects of FK506 and rapamycin on formation of the neural tube in chick embryos"Animal Science Journal. 73(3). 229-234 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kagami, H.: "Effect of the strain combination in donor and recipient for production efficiency of the W-bearing sperm in mixed-sex germline chimeric chickens"Animal Science Journal. 73(6). 453-456 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yazawa, S.: "Heart-selective expression of the chicken FK506-binding protein (FKBP) 12.6 gene during embryonic development"Developmental Dynamics. 226(1). 33-41 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ono, T., Li, HC.: "Restriction of proliferation of primordial germ cells by the irradiation of Japanese quail embryos with soft X-rays"Comparative Biochemistry and Physiology Part A. 130. 133-140 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ono, T.: "Ex Ovo Culture of Quail Embryos and its Application for Embryo Manipulation"Animal Science Journal. 72(5). 361-371 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ono, T., Li, HC.: "Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products"Journal of Poultry Science. 38(4). 308-316 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kagami, H.: "Developmental genetic analysis of avian primordial germ cells and the application to poultry biotechnology"2001 Symposium proceeding of Korean Society of Poultry Science. 27-34 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ono, T.: "Development of complete culture system for quail embryos and its application for embryo manipulation"2001 Symposium proceeding of Korean Society of Poultry Science. 47-55 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kagami, H.: "Strain preference in donor and recipient for production of W-bearing sperm in mixed-sex germline chimeric chickens"Comparative Biochemistry and Physiology Part A. 131(2). 287-292 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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