| Project/Area Number |
13470002
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
USHIKI Tatsuo NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40184999)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHI Osamu NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (10303124)
HITOMI Jiro Iwate Medical University, Professor, 医学部, 教授 (00218728)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | scanning probe microscopy / atomic force microscopy biomolecules / cellular function / 細胞機能 / 高分子観察 / 走査プローブ顕微鏡 / 高分子 / 液中観察 / 近接場光学顕微鏡 |
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| Research Abstract |
Scanning probe microscopes form a new family of microscopes, which scan a sharp probing tip over the sample surface, providing high resolution information about the surface characteristics of solid samples. This study was undertaken to apply these microscopes (including the atomic force microscope) to the observation of biological samples. For this purpose, we also investigated the preparation methods and observation techniques suitable for scanning probe microscopy. The obtained results are follows :
1.We studied the techniques for observing macromolecules such ad the deoxyribonucleic acid and myosin at high resolution with the atomic force microscope. These samples are usually mounted on a fleshly-cleaved mica and observed with the microscope in an air environment. In this study, we applied an FM control system to the visualization of macromolecule in a vacuum environment, and succeeded in observing them at high resolution.
2.We investigated the methods for observing biological samples (e.g., chromosomes) with the atomic force microscope in a liquid environment. While a contact mode is generally used for measuring soft samples in liquid, we investigate the method for operating the atomic force microscope in a dynamic mode. By adjusting adequately the operation parameters such as resonance frequency, amplitude and vibration voltage, we succeeded in obtaining clear images of chromosomes in liquid using a dynamic mode. Q-control technique for dynamic mode imaging was expected to be useful for the stable visualization of chromosomes without any fixation in a liquid environment.
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