| Project/Area Number |
13470042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MORI Shigeo University of Tokyo, The Institute of Medical Science, 医科学研究所, 教授 (30010424)
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| Co-Investigator(Kenkyū-buntansha) |
MORIYAMA Masatsugu The University of Tottori, Department of Molecular Biology, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90239707)
SATOH Hitoshi The University of Tokyo, Institute of Medical Science, Research Associate, 医科学研究所, 助手 (70183829)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2002: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | malignant lymphoma / germinal center / bcl-6 / methionine aminopeptidase 2 / snoRNA / B細胞 / pim-1 / met-ap2 / U50HG |
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| Research Abstract |
To subdivide the diffuse large B cell lymphoma which is a waste-basket type category in lymphoma differentiation, we intended to add some reliable molecules that contribute in such subdivision. We could identify three molecules that are expressed in diffuse large B cell lymphomas of germinal cents B cell type exclusively : U50HG, methionine aminopeptidase 2 (MetAP2) and cholesterine ester transfer protein (CET). In case of U50HG that is a protein non-coding snoRNA host gene, we found its transcript to be highly expressed in germinal center exclusively. Other two molecules, MetAP2 a CET were detected in germinal center B cells and their neoplastic counterparts intensely by their specific antibodies while very weakly on other B-and T-cell lymphomas. Those results suggest that the three gene products are very much useful for the subdivision of neoplastic B lymphocytes into germinal center type and other (may be activated B cell) type. Furthermore, we found a novel oncogene pim-1 to form a dominant negative type chimeric transcript on a certain B cell lymphoma that exhibit Bc16/Pim1 chromosomal translocation.
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