| Project/Area Number |
13470068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
SAITO Takashi Chiba University, Graduate School Of Medicine, Professor, 大学院・医学研究院, 教授 (50205655)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASAKI Sho Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (40312946)
高瀬 完 千葉大学, 大学院・医学研究院, 助手 (40333489)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2002: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | thymic differentiation / pre T cell receptor / pTα chain / knockout mouse / lipid raft / transgenic mouse / Th1 / Th2 / Th2 / Ebox / E2A / HEB / プロモーター / 転写制御 |
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| Research Abstract |
Signal regulation through the pre-TCR complex was analyzed n approaches ; one is to analyze a new molecule RIT which was cloned last year, two and the other is the analysis of pre-TCR signaling through lipid raft by preparing raft-residential chimeric molecule.
(1) RIT was cloned by DNA subtraction from pTα-expressing cells and is expressed only on CD25^+ immature thymocytes. RIT-KO mice were generated. By crossing with HY-specific TCR-Tg mice, RIT was found to modulate thymic selection.
(2) To prepare raft-residential protein, we made LAT-CD3εchimeric molecule. This molecule is indeed localized exclusively in lipid raft. By crossing with Rag-KO mice, the expression of LAT-CD3εaugumented development of DP thymocytes.
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