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Development of strategic therapy for severe hepatic failure using regenerative medicine-Isolation of hepatic stem cell expressing musashi-1 gene and cell therapy

Research Project

Project/Area Number 13470112
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionYamagata University

Principal Investigator

KAWATA Sumio  Yamagata Unv. Medical Sch., Professor, 医学部, 教授 (90183285)

Co-Investigator(Kenkyū-buntansha) SAITO Koji  Yamagata Unv. Medical Sch., Assistant Prof., 医学部, 講師 (90250919)
SAITO Takafumi  Yamagata Unv. Medical Sch., Assistent Prof., 医学部, 講師 (80250918)
TOGASHI Hitoshi  Yamagata Unv. Medical Sch., Associate Prof., 医学部, 助教授 (60192209)
WATANABE Hisayoshi  Yamagata Unv. Medical Sch., Assistant, 医学部, 助手 (00332536)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 2002: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsHepatic Stem cell / Musashi-1 gene / oval cell / hepatocytes / Hepatoma cell lines / 肝細胞 / oval cells
Research Abstract

We are trying to develop an innovative therapy for sever hepatic failure to transplant hepatic stem cell. In this therapy, it is an important step to isolate hepatic stem cell expressing musashi-1 gene.
In 2001 we established a cell line of oval cells, bipotent stem cells, from rat livers. This cell line expressed musashi-1 gene with AFP and CK-18 ones. With differenciation of the cell line by spheroid formation, the musashi-1 gene expression was suppressed. Moreover, three of seven human hepatoma cell lines expressed musashi-1 gene. This implied that musashi-1 gene might participate in differentiation of hepatic stem cells and hepatic carcinogenesis.
In 2002, we found that the musashi-1 gene regulated notch signalin via suppression of Numb gene expression. Also, role of musashi-1 gene in liver development is under investigation using knockout mice of the gene.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Shu H-J, Saito T, Kawata S, et al.: "Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines"Biochem.Biophys.Res.Commun.. 293. 150-154 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terui Y, Saito T, Kawata S, et al.: "Effect of angiotensin receptor antagonist on liver fibrosis of chronic hepatitis C"Hepatology. 36. 1022 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Lamivudine and rapid regeneration of the atrophic liver in decompensated cirrhosis due to Hepatitis B"Am J Gastroenterol. 97. 493-494 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saeki A, Tamura S, Kawata S, et al.: "Frequent impairment of the spindle assembly checkpoint in hepatocellular carcinoma"Cancer. 94. 2047-2054 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakon M, Nagano H, Kawata S et al.: "Combined intraarterial 5-FU and subcutaneous IFN-αtherapy for advanced hepatocellular carcinoma with tumor thrombi in the major portal branches"Cancer. 94. 435-442 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Home lactulose enema: prevention of hepatic encephalopathy at home"J.Gastroenterol. 97. 493-495 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shu H-J, Saito T, Kawata S, et al.: "Expression of the musashi-1 gene encoding the RNA-binding protein in human hepatoma cell lines"Biochem. Biophys. Res Commun.. 293. 150-154 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terui Y, Saito T, Kawata S, et al.: "Effect of angiotensin receptor antagonist on liver fibrosis of chronic hepatitis C"Hepatology. 36. 1022-1023 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Lamivudine and rapid regeneration of the atrophic liver in decompensated cirrhosis due to HBV"Am. J. Gastroenterol. 97. 493-494 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saeki A, Tamura S, Kawata S, et al.: "Frequency impairment of the spindle assembly checked point in hepatocellular carcinoma"Cancer. 94. 2047-2054 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakon M, Nagano H, Kawata S, et al.: "Combined intraarterial 5-FU and subcutaneous IFN-a therapy for advanced hepatocellular carcinoma"Cancer. 94. 435-442 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Home lactulose enema:prevention of hepatic encephalopathy at home"J. Gastroenterol. 37. 68-69 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shu H-J, Saito T, Kawata S, et al.: "Expression of the Musashi gene encoding the RNA-binding protein in human hepatoma cell lines"Biochem. Biophys. Res. Commun.. 293. 150-154 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terui Y, Saito T, Kawata S, et al.: "Effect of angiotensin receptor antagonist on liver fibrosis of chronic hepatitis C"Hepatology. 36. 1022 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Lamivadine and rapid regeneration of the atrophic liver in decompensated cirrhosis due to HBV"Am J Gastroenterol. 97. 493-494 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saeki A, Tamura S, Kawata S, et al.: "Fequency impairment of the spindle assembly check point in hepatocellular carcinoma"Cancer. 94. 2047-2054 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakon M, Nagano H, Kawata S. et al.: "Combined intraarterial 5-FU and subcutanious IFN-α therapy for advanced hepatocellular carcinoma"Cancer. 94. 435-442 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Home Inctulose enema : prevention of hepatic encephalopathy at home"J. Gastroenterol. 97. 493-495 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terui Y., Kawata S., et al.: "Effect of angiotensin receptor antagonist on plasma TGF-B1 levels and hepatis fibrosis in chronic hepatitis C"Hepatology. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Aoki M., Kawata S., et al.: "Clinical significance of a highly sensitive enzyme immunoassay of hepatitis B surface antigen"J.Med.Virol.. 66. 166-170 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kamada Y., Kawata S., et al.: "Vascular endothelial dysfunction resulting from L-arginine deficiency in lysinuric protein in tolerance"J.Clin.Invest. 108. 717-724 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kitada T., Kawata S., et al.: "The addition of bisecting N-acetylglucosamine residues to E-cadherin down regulates phosphonylation of B-catenin"J.Biol.Chem. 276. 475-480 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsushima H., Kawata S., et al.: "Circulating TGF-B1 as a predictor of liver metastasis after resection in colorectal cancer"Clin Cancer Res. 7. 1258-1262 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawata S., et al.: Brit J.Cancer. 84. 886-891 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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