| Project/Area Number |
13470205
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
SUGIYAMA Haruo Osaka University Medical School, Professor, 医学部, 教授 (70162906)
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| Co-Investigator(Kenkyū-buntansha) |
OJI Yasuke Osaka University Medical School, Assistant Professor, 医学部, 助手 (20294100)
OKA Yoshihiro Osaka University Graduate School of Medicine, Assistant Professer, 医学系研究科, 助手 (20273691)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2002: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2001: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Keywords | WT1 / leukemia / tumor marker / 32D / oncogene / leukemogenesis / single-cell RT-PCR / ウィルムス腫瘍遺伝子 / CD34 / G2 / M / cell cycle |
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| Research Abstract |
1. The wild-type WT1 gene is expressed in almost all leukemias and thus WT1mRNA is a pan-leukemia tumor marker.
2. The growth of leukemia cells was inhabited by the treatment with WT1 antisense oligomers.
3. Constitutive expression of the wild-type WT1 gene inhibited differentiation of 32D c13 myeloid progenitor cells and instead induced proliferation of the cells in response to G-CSF.
4. Normal myeloid progenitor cells transfected with the wild-type WT1 cDNA-integrated retroviral vetcor formed more CFU-G, CFU-M, and CFU-GM colonies compared to those transfected with control vector in response to G-CSF.
5. To determine the frequencies of WT1-expressing bone marrow cells, 1,632 CD34^+ human bone marrow progenitor cells were examined for the WT1 expression by using single-cell RT-PCR. Only 19 (1.2%) of the 1,632 CD34^+ cells expressed WT1mRMA and the WT1mRMA expression levels were similar between normal CD34^+ and leukemia single cells, suggesting that WT1-expressing CD34^+ cells were normal counterparts of WT1-expressing leukemia cells.
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