• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Medical treatment research by the gene cluster (EPR-1, dCK, and TP) which guides antineoplastic drug susceptibility reinforcement

Research Project

Project/Area Number 13470262
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionOsaka Medical College

Principal Investigator

TANIGAWA Nobuhiko  Osaka Medical College, Faculty of medicine, Professor, 医学部, 教授 (00111956)

Co-Investigator(Kenkyū-buntansha) MABUCHI Hideaki  Osaka Medical College, Faculty of medicine, Research Associate, 医学部, 助手 (80340545)
NOMURA Eiji  Osaka Medical College, Faculty of medicine, Assistant Professor, 医学部, 講師 (30288732)
YAMAMOTO Tetsuhisa  Osaka Medical College, Faculty of medicine, Research Associate, 医学部, 助手 (50330072)
篠原 尚  大阪医科大学, 医学部, 助手 (70319549)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2003: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥7,900,000 (Direct Cost: ¥7,900,000)
Keywordssurvivin / EPR-1 / HT29 / apoptosis / Adenovirus vector / retrovirus vector / dThdPase cDNA / dThdPase cDNA
Research Abstract

EPR-1 cDNA, a potential survivin antisense, was ligated into a plasmid vector (pMTN2) with metallothionein promoter. Then HT29 human colon adenocarcinoma cells (with positive survivin expression) were transfected with the pMTN2-EPR-1 plasmid vector. Apoptosis was induced in HT29-AS33 and HT29-AS49 subolones with high EPR-1 expression in the presence of Zinc and furthermore sensitivity to Cis-DDP and 5-FU in vitro was increased. Then, on the basis of future clinical development, EPR-1 expressing adenovirus vector (ad.CMV-EPR-1) was made and the in vivo antitumor treatment was assessed on nude mouse HT29 xenografts. When the xenografts were 8mm or more, Ad.CMV-EPR1 and Ad.CMV-LacZ were injected to the tumor bearing mice once. In former group tumor formation was reduced to 1/4 comparing to the controls. This, suggested that the effect was due to HT29 cell line apoptosis induction. In addition, efficacy of CDDP or 5-FU combination with Ad.CMV-EPR-1 was enhanced, with reduction in tumor vol … More ume of 25% and 16% respectively (Eur J Cancer, in Press). Moreover, we have previously shown that transfected cells with plasmid vector expressing dThdPase(TP) cDNA were more sensitive to 5'-DFUR and 5-FU(Br J Cancer 75,1997). Therefore, at the time of this study, TPcDNA (1.5 kb) was transferred to EcoRI site of a retroviral vector, -pNV7 and transfected murine adenocarcinoma cell lines of MC38-TP and MC38-Neo were obtained. In Western blot and ELISA analysis the expression of TP was detected in MC38-TP, but not in the controls and the growth rate of both subclones was identical. As increased sensitivity to 5-FU,5'-DFUR and Capecitabine was significantly observed in vitro for the former group, in vivo experiment was also studied using subcutaneously inoculated MC38-TP mouse models which confirmed the enhancement of 5'-DFUR and Capecitabine chemotherapeutic effect in MC38-TP (data in submission). Such chemosensitivity related gene studies may be useful to provide effective improvement in current cancer chemotherapy. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] M.Iwamoto, N.Tanigawa: "Prognostic value of tumor-infiltrating dendritic cells expressing CD38 in human breast carcinomas"Int J Cancer. 104. 92-97 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 谷川允彦: "抗癌剤感受性試験 テーラーメード癌化学療法への展望-テーラーメード癌化学療法"医薬ジャーナル. 39. 987-988 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 篠原 尚, 谷川允彦: "抗癌剤"消化器外科学レビュー〜最新主要文献と解説. 40-45 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 篠原 尚, 谷川允彦: "胃癌に対する化学療法レジメン-術後補助・進行/再発例-"臨床外科. 58(8). 1041-1046 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H, Okano, N.Tanigawa: "Concominant overexpression of cycloxygenase-2 and its prognostic drawback in human gastric carcinoma expressing HER-2 or lacking Smad 4"Clinical Cancer Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Meng, N.Tanigawa: "Prognostic significance and different properties of survivin splicing variants in gastric cancer"Cancer Letters. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamamoto: "The role of survivin as a new target of diagnosis and treatment in human cancer"Med Electron Microsc. 24. 207-212 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kobayashi: "Regression of lymph node metastasis by immuno-therapy using autologous breast tumorlysate pulsed dendritic cells : report of a case"Surgery Today. 31. 513-516 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Kawasaki: "Expression of survivin correlates with apoptosis, proliferation, and angiogenesis during human colorectal tumorigenesis"Cancer. 91(11). 2026-2032 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nohara: "Expression of cell-cycle regulator p27 is correlated to the prognosis and ER expression in breast carcinoma patients"Oncology. 60. 94-100 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Tanigawa: "Clinical study to identify specific characteristics of cancer newly developedin the remnant stomach"Gastric Cancer. 5. 23-28 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamamoto: "Down-regulation of survivin expression by induction of effector cell protease receptor-1 reduces tumor growth potential with increased sensitivity to anticancer agents in human colon cancer"Eur J Cancer. 38(17). 2316-2324 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Agui: "Comparative study of various biological parameters, including expression of survivin, between primary and metastatic human colonic adenocarcinomas"Anti Cancer Res. 22. 1769-1776 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Shinohara: "Expression of HER2 human gastric cancer cells directly correlates with antitumor activity of a recombinant disulfide-stabilized anti-HER2 immunotoxin"Journal of Surgical Research. 102. 169-177 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Okuzawa: "PSK, a protein-bound polysaccharide, overcomes defective maturation of dendritic cells exposed to tumor-derived factors in vitro"International Journal of Oncology. 20. 1189-1195 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Iwamoto: "Prognostic value of tumor-infiltrating dendritic cells expressing CD38 in human breast carcinomas"Int J Cancer. 104. 92-97 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H, Okano: "Concominant overexpression of cyclooxygenase-2 and its prognostic drawback in human gastric carcinoma expressing HER-2 or lacking Smad 4"Clinical Cancer Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Meng: "Prognostic significance and different properties of survivin splicing variants in gastric cancer"Cancer Letters. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Iwamoto, N.Tanigawa: "Prognostic value of tumor-infiltrating dendritic cells expressing CD38 in human breast carcinomas"Int J Cancer. 104(1). 92-97 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 谷川允彦: "抗癌剤感受性試験 テーラーメード癌化学療法への展望 -テーラーメード癌化学療法"医薬ジャーナル. 39(3). 987-988 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 篠原 尚, 谷川允彦: "抗癌剤"消化器外科学レビュー 〜最新主要文献と解説. 40-45 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 篠原 尚, 谷川允彦: "胃癌に対する化学療法レジメン -術後補助・進行/再発例-"臨床外科. 58(8). 1041-1046 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H, Okano, N.Tanigawa: "Concominant overexpression of cyclooxygenase-2 and its prognostic drawback in human gastric carcinoma expressing HER-2 or lacking Smad 4"Clinical Cancer Research. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Meng, N.Tanigawa: "Prognostic significance and different properties of survivin splicing variants in gastric cancer"Cancer Letters. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Tanigawa: "Clinical study to identify specific characteristics of cancer newly developed in the remnant stomach"Gastric Cancer. (5). 23-28 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yamamoto: "Down-regulation of survivin expression by induction of effector cell protease reseptor-1 reduces tumor growth potential with increased sensitivity to anticancer agents in human colon cancer"European Journal of Cancer. 38(17). 2316-2324 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Nishiguchi: "Sigmoidectomie Laparoscopique Avec Preservation Des Vaisseaux Sigmoidiens Dans Le Traitement Des Petits Cancer Du Sigmoide"Le Journal de Coelio-chirurgin. (41)3. 28-35 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Shinohara: "Laparoscopically-assisted pylorus-preserving gastrectomy with preservation of the vagus nerve"Eur J Surg. 168. 55-58 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Shinohara: "Expression of HER2 human gastric cancer cells directly correlates with antitumor activity of a recombinant disulfide-stabilized anti-HER2 immunotoxin"Journal of Surgical Research. 102. 169-177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Okuzawa: "PSK, a protein-bound polysaccharide, overcomes defective maturation of dendritic cells exposed to tumor-derived factors in vitro"International Journal of Oncology. 20. 1189-1195 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Shinohara: "Expression of HER2 Human Gastric Cancer Cells Directly Correlates with Antitumor Activity of a Recomnibant Disulfide-Stabilized Anti-HER2 Immunotoxin"Journal of Surgical Research. 102. 169-177 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yamamoto: "Down-regulation of survivin expression by induction of effector cell protease reseptor-1 reduces tumor growth potential with increased sensitivity to anticancer agents in human colon cancer"European Journal of Cancer. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Kawasaki: "Expression of Survivin Correlates with Apoptosis, Proliferation, and Angiogenesis during Human Colorectal Tumorigenesis."Cancer. 91(11). 2026-2032 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Kobayashi: "Regression of Lymph Node Metastasis by Immunotherapy Using Autologous Breast Tumor-Lysate Pulsed Dendritic Cells : Report of a Case."Surgery Today. 31. 513-516 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] E.Nomura: "Efficacy of intraperitoneal and intravenous chemotherapy and left upper Efficacy of intraperitoneal and intravenous chemotherapy and left upper abdominal evisceration for advanced gastric cancer"Gastric Cancer. 4. 75-82 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi