| Project/Area Number |
13470293
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
YOSHIMINE Toshiki Osaka Univ. Medical School, Professor, 医学系研究科, 教授 (00201046)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IZUMOTO Shuichi Osaka Univ. Medical School, Assistant Professor, 医学系研究科, 助手 (40324769)
MARUNO Motohiko Osaka Univ. Medical School, Associate Professor, 医学系研究科, 助教授 (10263287)
IKENAKA Kazuhiro National Institute for physiological Science, Okazaki Professor, 生理学研究所, 教授 (00144527)
森内 秀祐 大阪大学, 医学(系)研究科(研究院), 助手 (90322180)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥8,800,000 (Direct Cost: ¥8,800,000)
|
|---|
| Keywords | MAGE / Malignant glioma / proliferation / Glioblastoma / MAGE-E1 / protein / MAGE-E1蛋白 / グリオーマ / 細胞増殖 |
|---|
| Research Abstract |
MAGE-E1 is a member of the melanoma-associated antigen gene (MAGE) family that is up-regulated in malignant glioma relative to normal central nervous system cells. In this manuscript, we identified MAGE-E1 protein and studied its expression in glioma cells. Antibodies were produced in three rabbits immunized with a 14-mer peptide that matched a MAGE-E1 protein segment. Western blot analysis and immunocytochemistry of U87MG cells showed the protein to be localized in the cytoplasm. The protein was enriched around the nucleus in some of U87MG cells. MAGE-E1 protein expression was high in T98G cells cultured with 10% fetal bovine serum but low in growth-arrested cells that were serum-starved for 72 hours. The expression was up-regulated by stimulation of growth-arrested cells with 20% FBS. Western blot analysis and immunohistochemistry of gliomas resected from patients showed MAGE-E1 protein to be present at higher levels in glioblastomas than in diffuse astrocytomas. Our results suggest that MAGE-E1 play a role in the proliferation of glioma cells. MAGE-E1 might be a target molecule for treatment of malignant gliomas.
|
